Chelonodontops bengalensis (Tetraodontiformes: Tetraodontidae): A New Species of Puffer Fish from the Northern Bay of Bengal Based on Morphology and DNA Barcode

Abstract

The new puffer fish species Chelonodontops bengalensis (Pisces: Tetraodontidae) is described from two specimens collected on the southwest coast of the Bay of Bengal, Bangladesh. C. bengalensis is closely related to C. patoca (Hamilton, 1822), both species being large puffers with a peculiar, disc-shaped exposed nasal organ. The new species however differs from C. patoca with respect to dorsal rays (12 vs 10), anal rays (10 vs 8), pectoral rays (19 vs 15–16) and gill rakers (8 vs 7). C. bengalensis has spinules on its lateral sides but not on the caudal peduncle (vs. a patch of spinules on the back from behind the interorbital nearly to the dorsal fin and another on the throat and abdomen but absent on the lateral sides in C. patoca). Both the DNA barcode region of the mitochondrial COI gene and 16S rRNA gene region of C. bengalensis showed a clear monophyletic clade that distinguishes it as a separate species in the phylogenetic tree from other closely related puffer fish species. The COI barcode region shows a genetic divergence of 6 to 7.6% between C. bengalensis and C. patoca reported in GenBank from different parts of Asia.

This is a preview of subscription content, log in to check access.

References

  1. Allen GR, Randall JE (1977) Review of the sharpnose pufferfishes (subfamily Canthigasterinae) of the Indo-Pacific. Rec Aust Mus 30(17):475–517. doi:10.3853/j.0067-1975.30.1977.192

    Article  Google Scholar 

  2. Arunachalam M, Muralidharan M, Sivakumar P (2009) Extension of range of the marine puffer fish Chelonodon patoca (Tetraodontiformes: Tetraodontidae) to freshwater habitat of Kerala, India. J Threat Taxa 1(4):238–239. doi:10.11609/JoTT.o1828.238-9

    Article  Google Scholar 

  3. Day F (1876) The fishes of India: being a natural history of the fishes known to inhabit the seas and fresh waters of India, Burma, and Ceylon. Bernard Quaritch, London, 852 p

    Google Scholar 

  4. Dekkers WJ (1975) Review of the Asiatic freshwater puffers of the genus Tetraodon Linnaeus, 1758 (Pisces, Tetraodontiformes, Tetraodontidae). Bijdragen Dierkunde 45:87–142

    Google Scholar 

  5. Eschmeyer WN, Fricke R, Laan RVD (2018) Catalog of fishes: genera, species. http://researcharchive.calacademy.org/research/ ichthyology/catalog/fishcatmain.asp Accessed 03 Jan 2018

    Google Scholar 

  6. Froese R, Pauly D (2018) FishBase. http://www.fishbase.org Accessed 03 Jan 2018

    Google Scholar 

  7. Han KH, Baek J, Shin LS, Kim HJ, Yoon BI, Hwang JH, Lee SH (2017) Morphological description of three species of pufferfishes (Tetraodontidae) from India. Korean J Fish Aquat Sci 50(1):77–84. doi:10.5657/kfas.2017.0077

    Google Scholar 

  8. Hardy G, Hutchins B (1983) Monacanthidae. In: Fischer W, Bianchi G (eds) FAO species identification sheets for fishery purposes. Western Indian Ocean (Fishing Area 51). FAO Fisheries Department, Rome Hebert PDN

    Google Scholar 

  9. Ratnasingham S, Dewaard JR (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. P Roy Soc B-Biol Sci 270:S96–S99. doi:10.1098/rsbl.2003.0025

    Google Scholar 

  10. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  Google Scholar 

  11. Kimura S, Arshad A, Imamura H, Ghaffar MA (2015) Fishes of the northwestern Johor Strait, Peninsular Malaysia. Universiti Putra Malaysia Press and Mie University, 108 p

    Google Scholar 

  12. Kottelat M (2013) The fishes of the inland waters of Southeast Asia: a catalogue and core bibliography of the fishes known to occur in freshwaters, mangroves and estuaries. Raffles Bull Zool Suppl 27:1–663

    Google Scholar 

  13. Matsuura K (2001) Tetraodontidae. In: Carpenter KE, Niem VH (eds) The living marine resources of the Western Central Atlantic, Volume 3: Bony fishes part 2 (Opistognathidae to Molidae), sea turtles and marine mammals. FAO Species Identification Guide for Fishery Purposes and American Society of Ichthyologists and Herpetologists, Rome, pp 3954–3957

    Google Scholar 

  14. Matsuura K (2002) A review of two morphologically similar puffers, Chelonodon laticeps and C. patoca. Natl Sci Mus Monogr 22:175–180

    Google Scholar 

  15. Matsuura K (2015) Taxonomy and systematics of tetraodontiform fishes: a review focusing primarily on progress in the period from 1980 to 2014. Ichthyol Res 62:72–113

    Article  Google Scholar 

  16. Palumbi SR (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis DM, Moritz C, Mable BK (eds) Molecular systematics, 2nd edn. Sinauer Associates, Sunderland, pp 205–247

    Google Scholar 

  17. Rahman AKA (2005) Freshwater fishes of Bangladesh, 2nd edn. Zoological Society of Bangladesh, Dhaka, 230 p

    Google Scholar 

  18. Regan CT (1919) Fishes from Durban, Natal, collected by Messrs. H. W. Bell Marley and Romer Robinson. Ann Durban Mus 2(4):197–204

    Google Scholar 

  19. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

    Google Scholar 

  20. Shao K, Matsuura K, Jing L, Hardy G, Leis JL, Liu M (2014) Chelonodon patoca. The IUCN Red List of Threatened Species 2014: e.T172416A1341720. doi:10.2305/IUCN.UK.2014-3.RLTS.T172416A1341720.en

  21. Siddiqui KU, Islam MA, Kabir SMH, Ahmed M, Ahmed ATA, Rahman AKA, Haque EU, Ahmed ZU, Begum ZNT, Hasan MA, Khondker M, Rahman MM (2007) Encyclopedia of flora and fauna of Bangladesh, Vol. 23: freshwater fishes. Asiatic Society of Bangladesh, Dhaka, 300 p

    Google Scholar 

  22. Smith JLB (1958) Tetraodont fishes from South and East Africa. Ann Mag Nat Hist 13, 1(2): 156–160. doi:10.1080/00222935808650932

    Article  Google Scholar 

  23. Sujatha K, Padmavathi P (2015) Taxonomy of puffer fish (Pisces: Tetraodontidae) represented in the catches of Visakhapatnam, central eastern coast of India. J Mar Biol Assoc India 57(2):95–104. doi:10.26515/rzsi/v118/i1/2018/122386

    Google Scholar 

  24. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197

    Article  Google Scholar 

  25. Veeruraj A, Arumugam M, Ajithkumar T, Balasubramanian T (2011) Distribution of Tetraodontiformes (Family: Tetraodontidae) along the Parangipettai Coast, Southeast coast of India. Zootaxa 3015:1–12

    Article  Google Scholar 

  26. Ward RD, Zemlak TS, Innes BH, Last PR, Herbert PDN (2005) DNA barcoding Australia’s fish species. Philos T Roy Soc B 360:1847–1857

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Kazi Ahsan Habib.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Habib, K.A., Neogi, A.K., Oh, J. et al. Chelonodontops bengalensis (Tetraodontiformes: Tetraodontidae): A New Species of Puffer Fish from the Northern Bay of Bengal Based on Morphology and DNA Barcode. Ocean Sci. J. 54, 79–86 (2019). https://doi.org/10.1007/s12601-018-0054-7

Download citation

Keywords

  • Bangladesh
  • Bay of Bengal
  • new species
  • puffer fish
  • Sundarbans