Ocean Science Journal

, Volume 53, Issue 1, pp 81–90 | Cite as

Genus-specific PCR Primers Targeting Intracellular Parasite Euduboscquella (Dinoflagellata: Syndinea)

  • Jae-Ho Jung
  • Jung Min Choi
  • Young-Ok Kim


We designed a genus-specific primer pair targeting the intracellular parasite Euduboscquella. To increase target specificity and inhibit untargeted PCR, two nucleotides were added at the 3’ end of the reverse primer, one being a complementary nucleotide to the Euduboscquella-specific SNP (single-nucleotide polymorphism) and the other a deliberately mismatched nucleotide. Target specificity of the primer set was verified experimentally using PCR of two Euduboscquella species (positive controls) and 15 related species (negative controls composed of ciliates, diatoms and dinoflagellates), and analytical comparison with SILVA SSU rRNA gene database (release 119) in silico. In addition, we applied the Euduboscquella-specific primer set to four environmental samples previously determined by cytological staining to be either positive or negative for Euduboscquella. As expected, only positive controls and environmental samples known to contain Euduboscquella were successfully amplified by the primer set. An inferred SSU rRNA gene phylogeny placed environmental samples containing aloricate ciliates infected by Euduboscquella in a cluster discrete from Euduboscquella groups a–d previously reported from loricate, tintinnid ciliates.


marine parasite syndinean dinoflagellate SNP target-specific PCR primer ciliates 


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Copyright information

© Korea Institute of Ocean Science & Technology (KIOST) and the Korean Society of Oceanography (KSO) and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.South Sea Environment Research Center, South Sea Research InstituteKIOSTGeojeKorea
  2. 2.Department of Biology, College of Natural SciencesGangneung-Wonju National UniversityGangneungKorea

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