Abstract
Melon (Cucumis melo) is an important vegetable crop grown in different parts of India. Hitherto in India melons were reported to be attacked by RNA containing viruses only. During the present study a whitefly transmitted ssDNA containing begomovirus tomato leaf curl Palampur virus (ToLCPalV) was observed to infect muskmelon crop in Punjab. Infection of this virus leads to the production of yellows and leaf curl type symptoms in the infected plants. Exploitation of host plant resistance is an effective, economical, ecologically safe and durable approach of disease management. Along with these considerations 75 genotypes of muskmelon, snap melon and wild melon were screened against prevalent begomovirus at Vegetable Research Farm, Department of Vegetable Science, Punjab Agricultural University, Ludhiana during 2017 and 2018. Among 75 accessions screened under field conditions, 45 accessions were highly susceptible with disease incidence >60%, whereas, 10 entries showed resistance response with per cent disease incidence ranging between 20 to 40%, six accessions categorized under highly resistance response with 0.1–20% disease incidence and four accessions were found to be immune with no apparent symptoms. The 20 accessions showing resistance, highly resistance and immune response under field condition were further screened under artificial condition using mass inoculation technique against ToLCPalV along with four susceptible checks. Under artificial screening two accessions (SM/IC 267375 and WM-11) were highly resistant, seven accessions were resistant whereas, two accessions were moderately resistant. Based on screening of F2 population of crosses between MS-1(susceptible parent) x MM-Selection-103 (resistant parent) revealed monogenic dominant resistance in MM-Selection-103. This is the first record of a monogenic dominant resistant source in melon against a begomovirus.
Similar content being viewed by others
References
Arteaga, L., Alvarez, M., Alonso-Prados, J. M., Bernal, J. L., Garcia-Arenal, J. J., Lavina, F., Batlle, A., & Moriones, E. (1998). Occurrence, distribution, and relative incidence of mosaic viruses infecting field-grown melon in Spain. Plant Disease, 82, 979–982.
Dhkal, M., Sharma, A., & Kaur, G. (2020a). First report of tomato leaf curl New Delhi virus infecting muskmelon in India. Journal of Plant Pathology, 102, 1325.
Dhkal, M., Sharma, A., & Kaur, G. (2020b). First report of tomato leaf curl Palampur virus infecting muskmelon in India. Journal of Plant Pathology, 102, 1367.
Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11–15.
Esteva, J., & Nuez, E. (1992). Tolerance to a whitefly-transmitted virus causing muskmelon yellows disease in Spain. Theoretical and Applied Genetics, 84, 693–697.
Idris, A. M., Lujan, K. M., Martin, K., & Brown, J. K. (2008). Melon chlorotic leaf curl virus: Characterization and differential reassortment with closest relatives reveal adaptive virulence in the squash leaf curl virus clade and host shifting by the host-restricted bean calico mosaic virus. Journal of Virology, 82, 1959–1967.
Kheyr-Pour, A., Bananej, K., Dafalla, G. A., Caciagli, P., Noris, E., Ahoonmanesh, A., Lecoq, H., & Gronenborn, B. (2000). Watermelon chlorotic stunt virus from the Sudan and Iran: Sequence comparisons and identification of a whitefly-transmission determinant. Phytopathology, 90, 629–635.
Kumar, S., Kumar, S., Singh, M., Singh, A. K., & Rai, M. (2006). Identification of host plant resistance to pepper leaf curl virus in chilli (Capsicum spp). Scientia Horticulturae, 110, 359–361.
Lopez, C., Ferriol, M., & Pico, M. B. (2015). Mechanical transmission of tomato leaf curl New Delhi virus to cucurbit germplasm: Selection of tolerance sources in Cucumis melo. Euphytica, 204, 679–691.
Malik, A. H., Briddon, R. W., & Mansoor, S. (2011). Infectious clones of tomato leaf curl Palampur virus with a defective DNA B and their pseudorecombination with tomato leaf curl New Delhi virus. Virology Journal, 8, 173.
McCreight, J. D., & Wintermantel, W. M. (2011). Genetic resistance in melon PI 313970 to cucurbit yellow stunting disorder virus. Horticultural Science, 46, 1582–1587.
McCreight, J. D., Liu, H., & Turini, T. A. (2008). Genetic resistance to Cucurbit Leaf Crumple Virus in melon. Horticultural Science, 43, 122–126.
Mnari-Hattab, M., Zammouri, S., Belkadhi, M. S., Bellon Dona, D., Ben Nahia, E., & Hajlaoui, M. R. (2015). First report of tomato leaf curls New Delhi virus infecting cucurbits in Tunisia. New Disease Reports, 31, 21.
Monci, F., Garcia-Andres, S., Maldonado, J. A., & Moriones, E. (2005). Resistance to monopartite begomoviruses associated with the bean leaf crumple disease in Phaseolus vulgaris controlled by a single dominant gene. Phytopathology, 95, 819–826.
Rai, V. P., Kumar, R., Singh, S. P., Kumar, S., Kumar, S., Singh, M., & Rai, M. (2014). Monogenic recessive resistance to pepper leaf curl virus in an interspecific cross of Capsicum. Scientia Horticulturae, 172, 34–38.
Rajamony, L., More, T. A., Seshadri, V. S., & Varma, A. (1990). Reaction of muskmelon collections to cucumber green mottle mosaic virus. Journal of Phytopathology, 129, 237–244.
Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Disease, 77, 340–347.
Romay, G., Pitrat, M., Lecoq, H., Wipf-Scheibel, C., Millot, P., Girardot, G., & Desbiez, C. (2019). Resistance against melon chlorotic mosaic virus and tomato leaf curl New Delhi virus in melon. Plant Disease, 103, 2913–2919.
Saez, C., Esteras, C., Martinez, C., Ferriol, M., Dhillon, N. P. S., Lopez, C., & Pico, B. (2016). Resistance to tomato leaf curl New Delhi virus in melon is controlled by a major QTL located in chromosome 11. Plant Cell Reports, 36, 1571–1584.
Sharma, A., Kang, S. S., Kaur, S. I., & Gaikwad, A. (2012). Prevalence of cucurbit viruses in trans-gangetic plains of India. Journal of Research PAU, 49, 30–34.
Sharma, S., Kang, S. S., & Sharma, A. (2014). Source of resistance against cucumber mosaic virus and zucchini yellow mosaic virus in melons. Plant Disease Research, 19, 197–200.
Sufrin-Ringwald, T., & Lapidot, M. (2011). Characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: Squash leaf curl virus and watermelon chlorotic stunt virus. Phytopathology, 101, 281–289.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
Thakur, H., Jindal, S. K., Sharma, A., & Dhaliwal, M. S. (2018). A monogenic dominant resistance for leaf curl virus disease in chilli pepper (Capsicum annuum L.). Crop Protection, 116, 115–120.
Trisciuzzi, N., Silletti, M. R., Gallitelli, D., & Spano, R. (2018). First detection of tomato leaf curl New Delhi virus in melon and zucchini squash in southern Italy. Journal of Plant Pathology, 100, 149.
Venkataravanappa, V., Prasanna, H. C., Reddy, C. N. L., Chauhan, N., Shankarappa, K. S., & Reddy, M. K. (2021). Molecular characterization of recombinant bipartite begomovirus associated with mosaic and leaf curl disease of cucumber and muskmelon. Indian Phytopathology, 74, 775–785.
Venkataravanappa, V., Reddy, C. N. L., & Reddy, M. K. (2013). Begomovirus characterization and development of phenotypic and DNA-based diagnostics for screening of okra genotype resistance against Bhendi yellow vein mosaic virus. 3. Biotech, 3, 461–470.
Verlaan, M. G., Hutton, S. F., Ibrahe, R. M., Kormelink, R., Visser, R. G. F., Scott, J. W., Edwards, J. D., & Bai, Y. (2013). The tomato yellow leaf curl virus resistance genes ty-1 and ty-3 are allelic and code for DFDGD-class RNA dependent RNA polymerases. PLoS Genetics, 9(3), e1003399. https://doi.org/10.1371/journal.pgen.1003399
Wu, H. J., Li, M., Hong, N., Peng, B., & Gu, Q. S. (2020). Molecular and biological characterization of melon-infecting squash leaf curl China virus in China. Journal of Integrative Agriculture, 19(2), 570–577.
Yin, Y. Y., Fang, Q., Lu, X., Li, T. T., Ding, M., & Zhang, Z. K. (2014). Detection of watermelon silver mottle virus infecting watermelon in Yunnan, southwest of China. Journal of Plant Pathology, 96, 123.
Yousif, M. T., Kheyr-Pour, A., Gronenborn, B., Pitrat, M., & Dogimont, C. (2007). Sources of resistance to watermelon chlorotic stunt virus in melon. Plant Breeding, 126, 422–427.
Zitter, T. A., Hopkins, D. L., & Thomas, C. E. (1996). Compendium of cucurbit diseases.Pp 87. APS Press, St. Paul.
Acknowledgements
Authors are thankful to Department of Science and Technology, Govt. of India for INSPIRE fellowship to first author and facilities created under PURSE and FIST program. This study is the part of Ph.D thesis of Manmohan Dhkal.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
ESM 1
(DOCX 20 kb)
Rights and permissions
About this article
Cite this article
Dhkal, M., Sharma, A. & Sharma, S.P. Identification of resistance sources and monogenic dominant nature of resistance to tomato leaf curl Palampur virus in melons. Phytoparasitica 50, 757–767 (2022). https://doi.org/10.1007/s12600-022-00988-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12600-022-00988-2