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Intra species diversity of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in relation to geography and host plants affiliation in Uttarakhand Himalayan population, India

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Abstract

The gram pod borer, Helicoverpa armigera (Hübner) is the insect pest of global agricultural importance due to its wide host range and cosmopolitan distribution, which is supported by a strong genetic base. This paper presents genetic diversity of the pest population feeding on different hosts (inter-host) and geographic locations (inter-location) in Uttarakhand Himalayas, India and their micro-evolutionary consequences. A total of 21 inter-host and 17 inter-location populations were studied using both nuclear (SSR markers i.e., 1st tier) and mitochondrial genes (cytochrome b i.e., 2nd tier). In case of inter-host populations, grouping was observed in relation to crop season (Kharif and Rabi), sequential growing of the crops and association of crops with weeds and concurrence of crop growth stages. Interestingly, in both the nuclear and mitogenome analysis, the wheat population stood separate as a major cluster, which might be due to its adaptation towards the crop in recent past in the region. Although there is a differential grouping in SSR and cytb based clustering in inter-location populations, high altitude populations stand separate. The study illustrates ecological and evolutionary driven behavioural adaptations in H. armigera populations in Uttarakhand Himalayan population, India.

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All specimens were deposited to Department of Entomology, Pantnagar.

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Acknowledgements

Authors’ are thankful to Departments of Entomology, Genetics and Plant Breeding, College of Agriculture, GBPUAT, Pantnagar and Director, ICAR-Vivekananda Institute of Hill Agriculture, Almora, Uttarakhand and in-charge of CRC, VRC and HRC of GBPUAT, Pantnagar for providing us constant support during the entire research. This study is a part of the Ph.D. thesis conducted by the Dr. M.S. Sai Reddy, RPCAU, Bihar.

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  1. Department of Entomology, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, 848125, Bihar, India

    Morthala Shankara Sai Reddy & Somala Karthik

  2. Department of Entomology, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

    Meena Agnihotri

  3. Department of Genetics and Plant Breeding, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

    Jai Prakash Jaiswal

  4. Crop Protection Section, Vivekananda Institute of Hill Agriculture, Almora, 263601, Uttarakhand, India

    Avupati R. N. S. Subbanna

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Contributions

Sai Reddy MS: Planned and executed the research.

Agnihotri M: Supervised and guided the research.

Jaiswal JP: Molecular experimentation and Laboratory facilities.

Subbanna ARNS: Sample collection and DNA Extraction, Laboratory facilities, Molecular analysis.

Karthik S: Sample collection and data analysis.

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Correspondence to Morthala Shankara Sai Reddy.

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Reddy, M.S.S., Agnihotri, M., Jaiswal, J.P. et al. Intra species diversity of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in relation to geography and host plants affiliation in Uttarakhand Himalayan population, India. Phytoparasitica 50, 359–373 (2022). https://doi.org/10.1007/s12600-021-00972-2

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