Abstract
Severe incidence of striped flea beetle, Phyllotreta striolata (Fabricius) (Coleoptera: Chrysomelidae) in radish is being reported from Delhi National Capital Region and Haryana. The larvae and adult of this species were found to damage both roots and leaves of radish, respectively. Overall there was an average 63.02 percent leaf infestation and 53.96 percent root infestation in the radish fields surveyed, which was above the economic threshold level (25% infestation). Infestation was also observed on other cruciferous crops like turnip, mustard, cabbage and cauliflower in National Capital Region and Haryana. In the present study, we have furnished the species diagnosis, sexual dimorphism, nature of damage and illustrations of various stages of the pest. DNA barcoding was employed to confirm the species identity and molecular phylogenetic studies based on mt CO I sequences from across the world showed that there were two clusters; the Old World and the New World. The biology of the pest and the importance of studies on area specific population dynamics for formulating management strategies are discussed.
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References
Basu, C. R., Bhaumik, A. R., & Sengupta, T. (1981). Chrysomelidae (Coleoptera) of Tripura (India). Records of Zoological Survey of India, 78, 41–61.
Burgess, L. (1977). Flea beetles (Coleoptera: Chrysomelidae) attacking rape crop in the Canadian Prairie Provinces. Canadian Entomologist, 109, 21–32.
Capinera, J. (2001). Handbook of vegetable pests. Amsterdam: Elsevier. 800 pp.
Chakraborty, A. B., & Nei, M. (1977). Bottleneck effect on average heterozygosity and genetic distance with the stepwise mutation model. Evolution, 31, 347–356.
Cifuentes, D., Chynoweth, R., & Bielza, P. (2011). Genetic study of Mediterranean and South American populations of tomato leafminer, Tuta absoluta (Povolny, 1994) (Lepidoptera: Gelechiidae) using ribosomal and mitochondrial markers. Pest Management Science, 67, 1155–1162.
Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791.
Feng, H. T., Huang, Y. J., & Hsu, J. C. (2000). Insecticide susceptibility of cabbage flea beetle, Phyllotreta striolata (Fab.) in Taiwan. Plant Protection Bulletin Taiwan, 42, 123–146.
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNAprimers for amplification of mitochondrial cytochrome c oxidase subunit 1 from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.
Gissi, C., Iannelli, F., & Pesole, G. (2008). Evolution of the mitochondrial genome of Metazoa as exemplified by comparison of congeneric species. Heredity, 101, 301–320.
Hebert, P. D., Ratnasingham, S., & De Waard, J. R. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society: Biological Sciences, 270(suppl_1), S96–S99.
Heikertinger, F., & Csikt, E. (1939). Chrysomelidae (Halticinae 1) in Junk. Coleopterorum Catalogus, Berlin, 25(166), 1–336.
Hopkins, R. J., van Dam, N. M., & Van Loon, J. J. A. (2009). Role of glucosinolate in insect-plant relationships and multitrophic interactions. The Annual Review of Entomology, 54, 57–83.
Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G., & Gibson, T. J. (1998). Multiple sequence alignment with ClustalX [J]. Trends in Biochemical Sciences, 23, 403–405.
Jolivet, P., & Hawkewood, T. J. (1995). Host-plants of Chrysomelidae of the world: an essay about the relationships between the leaf beetles and their host-plants. Leiden: Backhuys Publishers. 281pp.
Konstantinov, A. S., & Vandenberg, N. J. (1996). Handbook of Palearctic flea beetles (Coleoptera: Chrysomelidae: Alticinae). Contributions on Entomology, International, 1, 236–439.
Kumar, P. (2001). Incidence of Flea Beetle, Phyllotreta downsei Baly on Radish. Karnataka Journal of Agricultural Sciences, 14(4), 1803–1804.
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.
Lee, C. F., Chang, H. Y., Wang, C. L., & Chen, W. S. (2011). A review of Phyllotreta Chevrolat in Taiwan (Coleoptera: Chrysomelidae: Galerucinae: Alticini). Zoological Studies, 50(4), 525–533.
Madder, D. J., & Stemeroff, M. (1988). The economics of insect control on wheat, corn, and canola, 1980–1985. Bulletin of the Entomological Society of Canada, 20(1), 1–22.
Margam, V. M., Coates, B. S., Ba, M. N., Sun, W., Binso-Dabire, C. L., Baoua, I., et al. (2011). Geographic distribution of phylogenetically-distinct legume pod borer, Maruca vitrata (Lepidoptera: Pyraloidea: Crambidae). Molecular Biology Reports, 38, 893–903.
Mason, J. A., & Kuhar, T. P. (2016). Evaluation of insecticides for the control of flea beetles in cabbage. Arthropod Management Tests, 41(1), tsw013.
Pivnick, K. A., Lamb, R. J., & Reed, D. (1992). Response of flea beetles, Phyllotreta spp., to mustard oils and nitriles in field trapping experiments. Journal of Chemical Ecology, 18, 863–873.
Rather, B. A., Hussain, B., & Mir, G. M. (2017). Seasonal incidence and biodiversity of flea beetles (Coleoptera: Alticinae) in a brassicaceous vegetable agro-ecosystem of Kashmir Valley. Entomological News, 127(3), 252–268.
Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Sarma, N. P., Singh, S., Sarma, D. K., Bhattacharyya, D. R., Kalita, M. C., Mohapatra, P. K., Dohutia, C., Mahanta, J., & Prakash, A. (2016). Mitochondrial DNA-based genetic diversity of Anopheles nivipes in North East India. Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 27, 4236–4239.
Scherer, G. (1969). Die Alticinae des indischen Subkontinentes (Coleoptera: Chrysomelidae). Pacific Insects Monograph, 22, 1–251.
Shashank, P. R., Chakravarthy, A. K., Raju, B. R., & Bhanu, K. R. (2014). DNA barcoding reveals the occurrence of cryptic species in host-associated population of Conogethes punctiferalis (Lepidoptera: Crambidae). Applied Entomology and Zoology, 49, 283–295.
Shashank, P. R., Twinkle, S., Chandrashekar, K., Meshram, N. M., Suroshe, S. S., & Bajracharya, A. S. R. (2018). Genetic homogeneity in South American tomato pinworm, Tuta absoluta: a new invasive pest to oriental region. 3 Biotech, 8, 350. https://doi.org/10.1007/s13205-018-1374-0.
Tamura, K. (1992). Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678–687.
Tansey, J. A., Dosdall, L. M., & Keddie, B. A. (2008). Phyllotreta cruciferae and Phyllotreta striolata responses to insecticidal seed treatments with different modes of action. Journal of Applied Entomology, 133(3), 201–209.
Trewick, S. A. (2000). Molecular evidence for dispersal rather than vicariance as the origin of flightless insect species on the Chatham Islands, New Zealand. Journal of Biogeography, 27, 1189–1200.
Ulmer, B. J., & Dosdall, L. M. (2006). Emergence of overwintered and new generation adults of the crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae). Crop Protection, 25, 23–30.
Varma, B. K. (1961). Bionomics of Phyllotreta crueiferae Goeze (Coleoptera: Chrysomelidae). Indian Journal of Agricultural Sciences, 31, 59–63.
Vogler, A. P., Desalle, R., Assmann, T., Knisley, C. B., & Schultz, T. D. (1993). Molecular population genetics of the endangered tiger beetle, Cicindela dorsalis (Coleoptera: Cicindelidae). Annals of the Entomological Society of America, 86, 142–152.
Westdal, P. H., & Romanow, W. (1972). Observations on the biology of the flea beetle, Phyllotreta cruciferae (Coleoptera: Chrysomelidae). Manitoba Entomology, 6, 35.
Wylie, H. G. (1979). Observations on distribution, seasonal life history, and abundance of flea beetles (Coleoptera: Chrysomelidae) that infest rape crops in Manitoba. Canadian Entomologist, 111, 1345–1353.
Yadav, D. C. (2010). Species composition of flea beetles infesting common cruciferous vegetables and their management in radish. Dissertation, Maharana Prathap University of Agriculture and Technology, Udaipur, Rajasthan.
Zepeda-Paulo, F., Simon, J. C., Rami´Rez, C. C., Fuentes-Contreras, E., Margaritopoulos, J. T., Wilson, A. C. C., et al. (2010). The invasion route for an insect pest species: the tobacco aphid in the New World. Molecular Ecology, 19, 4738–4752.
Acknowledgements
We thank Dr. KD Prathapan, Department of Entomology, College of Agriculture (Kerala Agricultural University) Thiruvananthapuram, Kerala for identification of flea beetles and for sharing few literatures. We are grateful to Head, Division of Entomology and Dr. RK Sharma, Principal Scientist and Dr. SR Sinha, Chief Technical Officer, Division of Entomology, Indian Agricultural Research Institute, New Delhi for their support during the initial surveys. The project was funded by Indian Agricultural Research Institute, New Delhi as a part of the in-house project entitled ‘Biosystematics of insect, fungi, bacteria and nematodes of economic importance (Code-CRSCIARISIL2014032264)’.
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All authors contributed to the study. Conceptualization: SS Anooj, PR Shashank, KV Raghavendra; Methodology: PR Shashank, KV Raghavendra, SS Anooj, V Vaibhav; Formal analysis and investigation: PR Shashank, SS Anooj, KV Raghavendra, C Nitya; Writing - original draft preparation: SS Anooj; Writing - review and editing: SS Anooj, PR Shashank, KV Raghavendra; Funding acquisition: PR Shashank, HR Sardana; Resources: SS Anooj, PR Shashank, KV Raghavendra, HR Sardana; Supervision: PR Shashank, SS Anooj. The first draft of the manuscript was written by SS Anooj and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Anooj, S.S., Raghavendra, K.V., Shashank, P.R. et al. An emerging pest of radish, striped flea beetle Phyllotreta striolata (Fabricius), from Northern India: incidence, diagnosis and molecular analysis. Phytoparasitica 48, 743–753 (2020). https://doi.org/10.1007/s12600-020-00825-4
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DOI: https://doi.org/10.1007/s12600-020-00825-4