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Determination of pathogenicity of Bursaphelenchus species on different pine species under natural conditions in Düzce

Abstract

The pinewood nematode, Bursaphelenchus xylophilus is a pathogen of conifer forest trees. This pest is characterized by the sudden death of infected pine trees. In this study, pathogenicity tests of six native Bursaphelenchus species (B. mucronatus, B. sexdentati, B. anamurius, B. vallesianus, B. andrassyi and B. hellenicus) on three pine species (Pinus pinaster, P. nigra and P. sylvestris) using two inoculum doses under natural conditions were conducted. For each treatment, 8 trees of each species were inoculated. For each tree species 8 trees were used as control groups. Inoculated trees were monitored for a year after inoculation. Monthly oleoresin flow measurements were carried out and external symptoms (foliage coloration) observed. Nematode inoculated trees did not die at the end of the study. The amount of oleoresin flow differed significantly among tree species. Inoculated Bursaphelenchus species did not cause any significant difference in the oleoresin flow between inoculated and uninoculated trees. We concluded that Bursaphelenchus species used in this study were not pathogenic to pine trees growing under natural forest stand conditions.

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References

  1. Abelleira, A., Picoaga, A., Mansilla, J. P., & Aguin, O. (2011). Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in northwestern Spain. Plant Disease, 95, 776–776.

    Article  CAS  PubMed  Google Scholar 

  2. Achim, B., & Zöfel, P. (2000). SPSS Version 10 Einführung in die moderne Datenanalyse unter Windows. Munich: Addison Wesley.

    Google Scholar 

  3. Akbulut, S., Vieira, P., Ryss, A., Yüksel, B., Keten, A., Mota, M., & Valadas, V. (2006). Preliminary survey of the pinewood nematode in Turkey. Bulletin OEPP/EPPO Bulletin, 36, 538–542.

    Article  Google Scholar 

  4. Akbulut, S., Braasch, H., Baysal, İ., Brandstetter, M., & Burgermeister, W. (2007a). Description of Bursaphelenchus anamurius sp. n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey. Nematology, 9, 859–867.

    Article  CAS  Google Scholar 

  5. Akbulut, S., Elekcioglu, I. H., & Keten, A. (2007b). First record of Bursaphelenchus vallesianus Braasch, Schonfeld, Polomski, & Burgermeister in Turkey. Turkish Journal of Agriculture and Forestry, 32, 273–279.

    Google Scholar 

  6. Akbulut, S., Yüksel, B., Serin, M., Baysal, İ., & Erdem, M. (2007c). Pathogenicity of Bursaphelenchus mucronatus in pine seedlings under greenhouse conditions. Turkish Journal of Agriculture and Forestry, 31, 169–173.

    Google Scholar 

  7. Akbulut, S., Vieira, P., Ryss, A., Valadas, V., Keten, A., & Mota, M. (2008). Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) species associated with Pinus species in northern Turkey. Helminthology, 45, 89–95.

    Article  CAS  Google Scholar 

  8. Akbulut, S., Yüksel, B., Serin, M., & Erdem, M. (2015). Comparison of pathogenic potential of Bursaphelenchus species on conifer seedlings between greenhouse and outdoor conditions. Phytoparasitica, 43, 209–214.

    Article  CAS  Google Scholar 

  9. Braasch, H. (1996). Pathogenitätstests mit Bursaphelenchus mucronatus an Kiefern und Fichtensämlingen in Deutschland. European Journal of Forest Pathology, 26, 205–216.

    Article  Google Scholar 

  10. Caroppo, S., Cavalli, M., Coniglio, D., & Ambrogioni, L. (2000). Pathogenicity studies with various Bursaphelenchus populations on conifer seedlings under controlled and open air conditions. Redia LXXXIII, 83, 61–75.

    Google Scholar 

  11. Dayı, M., & Akbulut, S. (2012). Pathogenicity testing of four Bursaphelenchus species on conifer seedlings under greenhouse conditions. Forest Pathology, 42, 212–219.

    Article  Google Scholar 

  12. Inacio, M. L., Nobrega, F. N., Vieira, P., Bonifacio, L., Naves, P., Sousa, E., & Mota, M. (2015). First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe. Forest Pathology, 45, 235–238.

    Article  Google Scholar 

  13. Kishi, Y. (1995). The pine wood nematode and the Japanese pine sawyer (p. 302). Tokyo: Thomas Company Ltd.

    Google Scholar 

  14. Kobayashi, F. (1978). Pine bark beetle problem in Japan, referring to the discovery of the pine wood nematode, Bursaphelenchus lignicolus (Mamiya & Kiyohara). Anzeiger fur Schadlingskunde, Pflanzenschutz, Umweltschultz, 51, 76–79.

    Article  Google Scholar 

  15. Kulinich, O. (2004) Surveys for the pine wood nematode in Russia. In M. Mota & P. Vieira (Eds.), The Pinewood Nematode, Bursaphelenchus xylophilus pp. 65–75. Proceedings of an International Workshop, University of Évora, Portugal. Nematol Monogr Persp 1, Brill, Leiden, (NL).

  16. Liebhold, A. M., MacDonald, W. L., Bergdahl, D., & Mastro, V. C. (1995). Invasion by exotic forest pests: A threat to forest ecosystems. Forest Science, 41, 1–49.

    Article  Google Scholar 

  17. Linit, M. J., & Tamura, H. (1987). Relative susceptibility of four pine species to infection by pinewood nematode. Journal of Nematology, 19, 44–50.

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Mamiya, Y. (1983). Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annual Review of Phytopathology, 21, 201–220.

    Article  CAS  PubMed  Google Scholar 

  19. Mamiya, Y. (1988). History of pine wilt disease in Japan. Journal of Nematology, 20, 219–226.

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Mamiya, Y. (1998). Review on the pathogenicity of Bursaphelenchus mucronatus. In K. Futai, K. Togashi, & T. Ikeda (Eds.) Sustainability of pine forests in relation to pine wilt and decline. Proceedings of International Symposium, pp. 57–64. Tokyo, 26–30 October,

  21. Mamiya, Y., & Enda, N. (1972). Transmission of Bursaphelenchus lignicolus (Nematoda: Aphelenchoididae) by Monochamus alternatus (Coleoptera: Cerambycidae). Nematologica, 18, 159–162.

    Article  Google Scholar 

  22. McNamara, D.G. (2004). Quarantine concerns about the methods used to demonstrate pathogenicity of Bursaphelenchus spp. In M. Mota & P. Vieira (Eds.) International workshop on pinewood nematode, Bursaphelenchus xylophilus Nematol Monogr Persp 1. pp. 187–197. Brill, Leiden,

  23. Melakeberhan, H., Rutherford, T. A., & Webster, J. M. (1992). Influence of temperature on reproduction of Bursaphelenchus xylophilus and Pinus sylvestris mortality. Nematologica, 38, 80–87.

    Article  Google Scholar 

  24. Mota, M. M., Braasch, H., Bravo, M. A., Penas, A. C., Burgermeister, W., Metge, K., & Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology, 1, 727–734.

    Article  Google Scholar 

  25. Oda, K. (1967). The applicability of measurement of oleoresin yield in determining the susceptibility of pine trees to beetle infestation. Forest Protection News, (Tokyo), 16, 263–266.

    Google Scholar 

  26. Rutherford, T. A., Mamiya, Y., & Webster, J. M. (1990). Nematode-induced pine wilt disease: Factors influencing its occurrence and distribution. Forest Science, 36, 145–155.

    Google Scholar 

  27. Sasaki, S., Odani, K., Nishiyama, Y., & Hayashi, Y. (1984). Development and recovery of pine wilt disease studied by tracing ascending sap flow marked with water soluble stains. Journal of Japanese Forestry Society, 66, 141–148.

    Google Scholar 

  28. Skarmoutsos, G., & Michalopoulos-Skarmoutsos, H. (2000). Pathogenicity of Bursaphelenchus sexdentati, Bursaphelenhus leoni, and Bursapelenchus hellenicus on European pine seedlings. Forest Pathology, 30, 149–156.

    Article  Google Scholar 

  29. Southey, J. F. (1986). Laboratory methods for working with plant and soil nematodes. In Ministry of Agriculture fisheries and food (Great Britain) technical bulletin (Vol. 2, pp. 1–202). London: Her Majesty’s Stationery Office.

    Google Scholar 

  30. Sugawa, T. (1982). Occurrence of traumatic resin canals and cytological changes of parenchyma cells in the stem of Japanese black pine suffering from pine wood nematodes. Journal of Japanese Forestry Society, 64, 112–116.

    Google Scholar 

  31. Suzuki, K., & Kiyohara, T. (1978). Influence of water stress on development of pine wilting disease caused by Bursaphelenchus lignicolus. European Journal of Forest Pathology, 8, 97–107.

    Article  Google Scholar 

  32. Tomminen, J. (1993). Pathogenicity with Bursaphelenchus mucronatus in scots pine in Finland. European Journal of Forest Pathology, 23, 236–243.

    Article  Google Scholar 

  33. Zhou, L. F., Chen, F. M., Wang, J. C., Pan, H. Y., & Ye, J. R. (2016a). Virulence of Bursaphelenchus mucronatus to pine seedlings and trees under field conditions. Forest Pathology, 1–9.

  34. Zhou, L. F., Chen, F. M., Pan, H. Y., Ye, J. R., Dong, X. J., Li, C. H., & Lin, F. L. (2016b). Identifying virulence-associated genes using transcriptomic and proteomic association analyses of the plant parasitic nematode Bursaphelenchus mucronatus. International Journal of Molecular Science, 17(9), 1492.

    Article  CAS  Google Scholar 

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Acknowledgements

This study is supported by Düzce University, Research Fund Project Number 2015.02.02.338. The authors greatly acknowledged the assistance and permission of General Directorate of Forestry, Bolu Regional Directorate of Forestry. The authors thank to Abdulmutalip Öztürk and Eyüp Öztürk for their help during field studies. The authors also kindly thank Dr. W. T. Stamps (University of Missouri-Columbia, USA) for his review of the manuscript.

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Correspondence to Süleyman Akbulut.

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Öztürk, N., Akbulut, S. & Baysal, İ. Determination of pathogenicity of Bursaphelenchus species on different pine species under natural conditions in Düzce. Phytoparasitica 47, 89–97 (2019). https://doi.org/10.1007/s12600-018-00708-9

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Keywords

  • Bursaphelenchus
  • Oleoresin flow
  • Pathogenicity
  • Pinus spp.