The toxins produced by Rhizoctonia solani are important causal agents of rice sheath blight. Effective detection of such toxins could improve the determination of the virulence of this agronomically important fungal pathogen. As such, the objective of the current study was to investigate the use of a variety of plant species [annual sowthistle (Sonchus oleraceus L.), Chinese cabbage (Brassica chinensis), spinach (Spinacia oleracea L.), lettuce (Lactuca sativa L. var. sativa), long leaf lettuce (Lactuca sativa var. ramosa Hort) and tobacco (Nicotiana tabacum)] for qualitative detection of R. solani crude toxins (RHCT) to replace the current rice leaf sheath based assay. This is constrained as rice plant takes long time to grow before the leaf sheath can be harvested From the initial screen, it was found that detached lettuce leaves provided the best alternative to rice material. Quantitative determination of RHCT activity by the phosphorus extravasation method was then performed on both rice (Oryza sativa L.) and lettuce. The results demonstrated that the detached lettuce leaves had the advantages of fast onset of symptoms, high sensitivity and non-perishability after inoculation. The quantity of phosphorus exosmosis observed in both lettuce leaves and rice leaf sheaths were significantly positively correlated. These data indicate that lettuce leaves can be used as a substitute material for rice leaf sheaths, with which to study the RHCT both qualitatively and quantitatively. The current study provides a new way to qualitatively and quantitatively detect RHCT.
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Brooks, S. A. (2007). Sensitivity to a phytotoxin from Rhizoctonia solani correlates with sheath blight susceptibility in rice. Phytopathology, 97, 1207–1212.
Chaudhary, R. N., Singburaudom, N., Sommartya, T., Sarobol, E. (1997). Inoculation techniques of crude extract toxin produced by Pyricularia oryzae Cav. Kasetsart Journal Natural Sciences, 31(3), 368–380.
Chen, X., Pan, C., Meng, L., Tong, Y., Zuo, S., Pan, X., & Xu, J. (2011). Separation, purification, and preliminary composition analysis of the toxin of Rhizoctonia solani, the pathogen of rice sheath blight. Journal of Yangzhou University, 32, 44–48.
Kumar, K. V. K., Reddy, M. S., Kloepper, J. W., Lawrence, K. S., Groth, D. E., & Miller, M. E. (2009). Sheath blight disease of rice (Oryza sativa L.)-an overview. Biosciences, Biotechnology Research Asia, 6, 465–480.
Sheng, Q., Ling, W., Ran, W., Bing, Z., Chen, S., Zhu, Y., Dong, Y., & An, C. (2001). Bioassay of the herbicidal activity of AAC-toxin produced by Alternaria alternata isolated from Ageratina adenophora. Weed Technology, 24, 197–201.
Tao, L. I., Xue-Jun, L. U., Liao, X. L., & Xiao-Ming, O. U. (2010). Occurrence and control strategy of rice sheath blight. Acta Agriculturae Jiangxi, 22, 91–93.
Yang, Y. Q., Yang, M. L., Li, M. H., Jia, Y., Zhang, D. T., & Zhou, E. (2011). Isolation and characterization of a phytotoxin from Rhizoctonia solani, the causal agent of rice sheath blight. Asian Journal of Chemistry, 23, 3500–3508.
Yugander, A., Ladhalakshmi, D., Prakasham, V., Mangrauthia, S. K., Prasad, M. S., Krishnaveni, D., Madhav, M. S., Sundaram, R. M., & Lara, G. S. (2015). Pathogenic and genetic variation among the isolates of Rhizoctonia solani, (AG1-IA), the rice sheath blight pathogen. Journal of Phytopathology, 163, 465–474.
Zhang, H. X., Nan, X. U., Yang, J. R., & Yan, L. I. (2008). Extraction and bioassay of the toxin produced by verticillium dahliae. Acta Agriculturae Boreali-Occidentalis Sinica, 17, 312–314.
Zuo, S. M. (2006). Establishment and inprovement of inoculation technique and rating system in researching rice sheath blight resistance in field. Journal of Yangzhou University, 27, 57–61.
Zuo, S. M., Zhang, Y. F., Chen, Z. X., & Chen, X. J. A. (2010). Current progress in genetics and breeding in resistance to rice sheath blight. Scientia Sinica, 40, 1014–1023.
Zuo, S. M., Chen, X. J., Chen, H. Q., Yan, X. U., Zhang, J. H., Chen, Y., Chen, Z. X., Tong, Y. H., Xu, J. Y., & Pan, X. B. (2014). Defense response and physiological difference of rice cultivars with different sheath blight resistance levels to the toxins produced by Rhizoctonia solani. Chinese Journal of Rice Science, 28, 551–558.
We thank Dr. Timothy C. Baldwin, Reader in Plant Cell Biology, Faculty of Science and Engineering in University of Wolverhampton of UK for his critical evaluation and editing of the original manuscript. This research was supported by National Key R&D Program of China (No. 2017YFD0200400) and the National Natural Scientific fund (30660006, 31160352).
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Ren, S.F., Zhang, Z.Y., Dong, W.H. et al. The application of lettuce in the qualitative and quantitative detection of Rhizoctonia Solani AG- 1 IA toxins. Phytoparasitica 45, 583–589 (2017). https://doi.org/10.1007/s12600-017-0614-x
- Rhizoctonia crude toxins (RHCT)
- Qualitative detection
- Quantitative detection