, Volume 44, Issue 4, pp 549–561 | Cite as

Characterization of Colletotrichum gloeosporioides, as the main causal agent of citrus anthracnose, and C. karstii as species preferentially associated with lemon twig dieback in Portugal

  • Ana Paula Ramos
  • Pedro TalhinhasEmail author
  • S. Sreenivasaprasad
  • Helena Oliveira


In the last two decades significant losses in citrus production in Portugal related to anthracnose symptoms have been recorded. These symptoms were attributed to Colletotrichum gloeosporioides, but preliminary population diversity evidence suggested that other Colletotrichum species could be involved in the disease. In this work, a field survey of the main citrus growing areas in Portugal was conducted and the pathogenicity of a group of Colletotrichum spp. isolates was studied along with morphological and genetic variability characterization [including Inter-Simple Sequence Repeats (ISSR) profiles and DNA sequence data of rDNA-ITS and β-tubulin 2 gene regions]. Colletotrichum karstii (from the C. boninense species complex) and C. gloeosporioides were identified from symptoms on leaves, branches, flowers and fruits of several citrus cultivars. However, C. acutatum, the species most commonly associated with citrus anthracnose in the Americas, was never detected. While C. gloeosporioides was isolated at higher frequency overall (87 %), C. karstii was more frequent in branches and leaves of lemon in specific geographic locations. Only C. gloeosporioides was detected in flowers. Colletotrichum karstii and C. gloeosporioides were pathogenic to sweet orange flowers and fruits and to leaves of sweet orange, mandarin and lemon, while reference C. acutatum citrus isolates were pathogenic to Key lime flowers and leaves.


Citrus anthracnose Colletotrichum boninense species complex Colletotrichum karstii Colletotrichum gloeosporioides Portugal 



Fundação Calouste Gulbenkian (Portugal) funded Grant 72426/2005 attributed to APR. Fundação para a Ciência e a Tecnologia (Portugal) funded grant SFRH/BPD/88994/2012 attributed to PT. The research reported in this work was conducted at Instituto Superior de Agronomia, Universidade de Lisboa (Lisbon, Portugal) and at Warwick HRI, University of Warwick (Wellesbourne, United Kingdom).


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de AgronomiaUniversidade de LisboaLisbonPortugal
  2. 2.Department of Life Sciences & Institute of Biomedical and Environmental Science and TechnologyUniversity of BedfordshireLutonUK

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