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Potato remorin gene StREMa4 cloning and its spatiotemporal expression pattern under Ralstonia solanacearum and plant hormones treatment

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Abstract

Plant-specific remorin genes have been identified in angiosperms, gymnosperms, ferns, and mosses. Several remorin genes are highly conserved in plant genomes, and their basic characteristics have been determined. Remorins have multiple biological functions, including in antibacterial defense, signal transduction, damage repair, and resistance to environmental stresses. In the present study, a full-length cDNA clone of the StREMa4 remorin gene was isolated from Ralstonia solanacearum-infected potato (Solanum tuberosum L.) cultivar ‘ED13’ plants through the rapid amplification of cDNA ends. Sequence analyses revealed that StREMa4 comprised 803 bp, including a 591 bp open reading frame that encoded a protein consisting of 197 amino acids. The StREMa4 protein was highly homologous to remorins from potato and other Solanaceae species. Real-time PCR analyses revealed that in addition to being up-regulated by Ralstonia solanacearum, StREMa4 expression was induced by exogenous hormones (i.e., salicylic acid, methyl jasmonate, and abscisic acid), with some differences in the expression patterns. Tissue localization analyses indicated that StREMa4 expression was tissue-specific, occurring primarily in the phloem of stem and leaf tissues.

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Acknowledgments

We would like to thank Bo Yu (Cell Laboratory, Shanxi normal university) for technical services; thank the institutes of Vegetables & Flowers and Plant Protection of the Chinese Academy of Agricultural Sciences for materials. This work was supported by the project of National Natural Science Foundation of China (31271774).

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Authors and Affiliations

  1. College of Life Science, Shanxi Normal University, Linfen, 041004, People’s Republic of China

    Chao-Yue Kong, Yong-ping Luo, Ting-Ting Duan, Zhen Xue, Xiu-Dan Gao, Xin Zhao & Gang Gao

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  1. Chao-Yue Kong
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  2. Yong-ping Luo
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  3. Ting-Ting Duan
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  4. Zhen Xue
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  7. Gang Gao
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Correspondence to Gang Gao.

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Kong, CY., Luo, Yp., Duan, TT. et al. Potato remorin gene StREMa4 cloning and its spatiotemporal expression pattern under Ralstonia solanacearum and plant hormones treatment. Phytoparasitica 44, 575–584 (2016). https://doi.org/10.1007/s12600-016-0536-z

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