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Phytoparasitica

, Volume 44, Issue 3, pp 283–293 | Cite as

Etiological and epidemiological concerns about Pepper root and lower stem rot caused by Fusarium oxysporum f. sp. radicis-capsici f. sp. nova

  • Tomás Lomas-Cano
  • Amalia Boix-Ruiz
  • Miguel de Cara-GarcíaEmail author
  • José Ignacio Marín-Guirao
  • Daniel Palmero-Llamas
  • Francisco Camacho-Ferre
  • Julio César Tello-Marquina
Article

Abstract

Inoculum sources of Fusarium oxysporum f. sp. radicis-capsici and host susceptibility were studied in commercial pepper cultivars and related Capsicum spp. The pathogen could not be identified in irrigation water or in the microbiologically enriched potting medium, or associated with the seeds used in the nursery where the disease was initially reported in Spain. Although the disease was asymptomatic on older pepper plants, pathogenic strains could still be isolated from the soil of those greenhouses where the disease had been previously observed. Host susceptibility in commercial pepper cultivars and related Capsicum spp. was studied. All pepper cultivars tested (n = 19), including three rootstocks, developed symptoms when inoculated with Fusarium oxysporum f. sp. radicis-capsici. Three C. baccatum accessions and one C. annuum accession exhibited resistance, whereas two C. chinense accessions showed only partial resistance. The disease symptoms could not be distinguished from those caused by one F. oxysporum f. sp. capsici isolate, and molecular analysis also showed identical tef sequences for both formae speciales.

Keywords

Breeding Inoculum preservation Resistance Soil 

References

  1. Barnett, H. L., & Hunter, B. B. (1972). Illustrated genera of imperfecti fungi (4th ed.). New York: Macmillan Publishing Company.Google Scholar
  2. Black, L. L. (2003). Fusarium wilt. Pages 14–15 in. Compendium of Pepper Diseases. Perezny, K., Roberts, P. D., Murphy, J. F., Goldberg, N. P., eds. American Phytopathological Society Press.Google Scholar
  3. Black, L. L., Green, S. K., Hartman, G. L., Poulos, J. M. (1993). Enfermedades del Chile. Una Guía de Campo. Ed. Centro Asiatico de Investigación y Desarrollo Vegetal (AVRDC).Google Scholar
  4. Boswell V. R., Doolittle S. P., Pultz L. M. (1952). Pepper production, disease and insect control. Farmers’ Bulletin n° 2051. United States Department of Agriculture. 30 ppGoogle Scholar
  5. Couteaudier, Y., & Alabouvette, C. (1981). Fusarium wilt disease in soiless cultures. Acta Horticulturae, 26, 153–157.Google Scholar
  6. Crawford, R. F. (1934). The etiology and control of chile wilt, produced by Fusarium annuum. New Mexico Agricultural Experiment Station Technical Bulletin, 223.Google Scholar
  7. De Cara, M., Pérez, M., Santos, M., Tello, J. C., Palmero, D., & Gómez, J. (2011). Inoculum sources and preservation in soils of Phytophthora parasitica from cherry tomato in continental crop areas in Southeast Spain. Acta Horticulturae, 914, 105–108.CrossRefGoogle Scholar
  8. Fernández, R., Trapero, A., Domínguez, J. (2010). Experimentación en Agricultura. Consejería de Agricultura y Pesca. Junta de Andalucía. Sevilla.Google Scholar
  9. Goidanich G. (1964). Manuale de Patologia Vegetale (vol II). Ed: Edizioni Agricole Bologna.Google Scholar
  10. Gómez Váquez, J., & Tello Marquina, J. C. (2000). Las semillas de melón (Cucumis melo L.) portadoras de diversos patotipos de Fusarium oxysporum f. sp. melonis. Boletin de Sanidad Vegetal Plagas, 26(1), 35–45.Google Scholar
  11. Gómez Vázquez, J., Mendiola Puig, A., & Tello, J. C. (2000). Estado sanitario de los sustratos utilizados para la producción de plántulas en Almería. Actas de Horticultura, 32, 229–240.Google Scholar
  12. Gómez-Tenorio, M. A., Zanón, M. J., De Cara, M., Lupión, B., & Tello, J. C. (2015). Efficacy of dimethyl disulfide (DMDS) against Meloidogyne sp. and three formae speciales of Fusarium oxysporum under controlled conditions. Crop Protection, 78, 263–269.CrossRefGoogle Scholar
  13. Govindaswamy, C. V. (1963). Studies on root-rot diseases of eggplant, Chilli and tomato in the Madras State. Journal of the Madras University, 23(3), 203–230.Google Scholar
  14. Guirado Moya, M. L., Aguilar, M. I., Blanco, R., Kenig, A., Gómez, J., & Tello, J. C. (2004). Fusarium wilt on sweet basil: cause and sources in Southeastern Spain. Phytoparasitica, 32(4), 395–401.CrossRefGoogle Scholar
  15. Hirano, Y., & Arie, T. (2006). PCR-based differentiation of Fusarium oxysporum f. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici. Journal of General Plant Pathology, 72, 73–283.CrossRefGoogle Scholar
  16. Jarvis, W. R., & Shoemaker, R. A. (1978). Taxonomic status of Fusarium oxysporum causing Foot and Root Rot of Tomato. Phytopathology, 68, 1679–1680.CrossRefGoogle Scholar
  17. Joffe, A. Z., & Palti, J. (1972). Fusarium species of the Martiella section in Israel. Phytopathologische Zeitschrift, 73, 123–148.CrossRefGoogle Scholar
  18. Jones, M. M. (1992). Fusarium wilt of pepper: Response of Capsicum spp. accessions to Fusarium oxysporum f. sp. capsici and analysis of vegetative compatibility. M. Sc. dissertation, Louisiana State University.Google Scholar
  19. Klein, E., Ofek, M., Katan, J., Minz, D., & Gamliel, A. (2013). Soil suppressiveness to Fusarium disease: shifts in root microbiome associated with reduction of pathogen root colonization. Phytopathology, 103, 23–33.CrossRefPubMedGoogle Scholar
  20. Komada, H. (1975). Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soils. Review of Plant Protection Research, 8, 114–125.Google Scholar
  21. Leonian, L. H. (1919). Fusarium wilt of chile pepper. New Mexico Agricultural Experiment Station Technical Bulletin, 121. Google Scholar
  22. Leslie J. F., Summerell B. A. (2006). The Fusarium Laboratory Manual. Blackwell Publishing.Google Scholar
  23. Lievens, B., Claes, L., Vakalounakis, D. J., Vanachter, A. C. R. C., & Thomma, B. P. H. J. (2007). A robust identification and detection assay to discriminate the cucumber pathogens Fusarium oxysporum f. sp. cucumerinum and f. sp. radicis-cucumerinum. Environmental Microbiology, 9, 2145–2161.CrossRefPubMedGoogle Scholar
  24. Lomas-Cano, T., Palmero-Llamas, D., de Cara, M., García-Rodríguez, C., Boix-Ruiz, A., Camacho-Ferre, F., & Tello-Marquina, J. C. (2014). First report of Fusarium oxysporum of sweet pepper seedlings in Almeria, Spain. Plant Disease, 98(10), 1435–1436.CrossRefGoogle Scholar
  25. Miller, S. A., Rowe, R. C., Riedel, R. M. (1996). Fusarium and Verticillium wilts of tomato, potato, pepper and eggplant. The Ohio State Univ. Exten. Fact. HYG-3122-96.Google Scholar
  26. Montemartini, L. (1907). L’avvizzimiento o la malattia dei peperono (Capsicum annuum L.) a Voghera. Rivista di Patologia Vegetale, 2(17), 257–259.Google Scholar
  27. Nelson, P. E., Toussoun, T. A., Marasas, W. F. O. (1983). Fusarium Species. An Illustrated Manual for Identification. The Pennsylvania State University Press.Google Scholar
  28. Ortuño Martínez, A., Parra Gilabert, M., Cánovas Candel, F. L., Hernansaez Rabay, A. (1972). La Fusariosis del Pimiento para Pimentón e Influencia del Maneb en el Cultivo y Producción de esta Solanácea. Instituto de Orientación y Asistencia Técnica del Sureste. Centro de Edafología y Biología Aplicada del Segura, Murcia (España).Google Scholar
  29. Parke, J. L., Grau, C. R. (1993). Aphanomyces. In: Methods for Research on Soilborne Phytopathogenic Fungi. Singleton, L., L., Mihail, J. D., Rush, C. M., eds. American Phytopathological Society Press. Pages 27–30Google Scholar
  30. Pérez-Hernández, A., Serrano-Alonso, Y., Aguilar-Pérez, M. I., Gómez-Uroz, R., & Gómez-Vázquez, J. (2014). Damping-off and root rot of pepper caused by Fusarium oxysporum in Almería province, Spain. Plant Disease, 98(8), 1159.CrossRefGoogle Scholar
  31. Pontis, R. E. (1940). El “marchitamiento” del pimiento (Capsicum annuum) en la provincia de Mendoza. Revista Argentina de Agronomía, 7(2), 113–127.Google Scholar
  32. Rabinowitch, H. D., Cohen R. (2012). Genetics and breeding for resistance and grafting for protection against Fusarium oxysporum wilts. In: Fusarium Wilts of Greenhouse vegetable and Ornamental Crops. Gullino, L., Katan, J., Garibaldi, A., eds. American Phytopathological Society Press.Google Scholar
  33. Rahin A. A., Sharif F. M. (1985). A study of pepper wilt in northern Iraq. In: Ecology and Management of Soilborne Plant Pathogens. Parker C. A., Rovira A. D., Moore K. J., Wong P. T. W., Kollmorgen J. F. Eds., American Phytopathological Society. 59–62Google Scholar
  34. Rekah, Y., Shtienberg, D., & Katan, J. (2000). Disease development following infectioon of tomato and basil foliage by airborne conidia of the soilborne pathogens Fusarium oxysporum f. sp. radicis-lycopersici and Fusarium oxysporum f. sp. Basilici. Phytopathology, 90, 1322–1329.CrossRefPubMedGoogle Scholar
  35. Rivelli, V. C. (1989). A wilt of pepper incited by Fusarium oxysporum f. sp. capsici forma specialis nova. M. Sc. dissertation, Louisiana State University.Google Scholar
  36. Tello Marquina, J. C., Lacasa Plasencia, A. (1990). Fusarium oxysporum en los Cultivos Intensivos del Litoral Mediterráneo de España. Fase Parasitaria (Fusariosis Vasculares del Tomate y del Clavel) y no Parasitaria. Bol. San. Veg. Plagas, 19. Ministerio de Agricultura y Pesca, Spain.Google Scholar
  37. Tello, J. C., Varés, F., Lacasa, A. (1991). Análisis de muestras. Pages 39–48, in: Manual de Laboratorio. Diagnóstico de Hongos, Bacterias y Nematodos Fitopatógenos. VV. AA. Ministerio de Agricultura y Pesca, Spain.Google Scholar
  38. Vakalounakis, D. J. (1996). Root and stem rot of cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum f. sp. nov. Plant Disease, 80, 313–316.CrossRefGoogle Scholar
  39. Weimer, J. L. (1944). Some root rot and foot rot of lupines in the southeastern part of the United States. Journal of Agricultural Research, 68, 441–457.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Tomás Lomas-Cano
    • 1
  • Amalia Boix-Ruiz
    • 1
  • Miguel de Cara-García
    • 3
    Email author
  • José Ignacio Marín-Guirao
    • 1
  • Daniel Palmero-Llamas
    • 2
  • Francisco Camacho-Ferre
    • 1
  • Julio César Tello-Marquina
    • 1
  1. 1.Grupo de Investigación AGR-200 “Producción Vegetal en Sistemas de Cultivo Mediterráneos”Universidad de AlmeríaAlmeríaSpain
  2. 2.Universidad Politécnica de Madrid. E.U.I.T.A.MadridSpain
  3. 3.Crop Protection, IFAPA-La MojoneraLa MojoneraSpain

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