Abstract
Phytoplasma belonging to subgroup 16SrII-A and identified as “Candidatus Phytoplasma australasiae”- was found constantly associated with phyllody of Pedalium murex, a common medicinal cum succulent weed in coconut ecosystem prevalent in South India. Infected P. murex showed stunted growth with reduced leaf size, shortened internodes and the characteristic transformation of floral parts into leafy structures typical to that of phytoplasma disease. This weed could possibly serve as a reservoir of sesamum phyllody in this region but no link with root (wilt) disease in coconut could be established based on molecular characterization studies. This is the first report of the association of 16SrII-A group phytoplasma with pedalium phyllody from the world.
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References
Abraham, E. V., Natarajan, K., & Murugaesan, M. (1977). Damage by pests and phyllody to S. indicum in relation to time of sowing. Madras Agricultural Journal, 64, 298–301.
Akhtar, K. P., Dickinson, M., Sarwar, G., Jamil, F. F., & Haq, M. A. (2008). First report on the association of a 16SrII phytoplasma with sesame phyllody in Pakistan. Plant Pathology, 57, 771.
Altschul, S. F., Gush, W., Miller, W., Myers, W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.
Deng, S., & Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiology Methods, 14, 53–61.
Gundersen, D. E., & Lee, I.-M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35, 144–151.
Lodhi, M. A., Ye, G. N., Weeden, N. F., & Reisch, B. I. (1994). A simple and efficient method for DNA extraction from grape vine cultivars, Vitis species and Ampelopsis. Plant Molecular Biology Reporter, 12, 6–13.
Manimekalai, R., Soumya, V. P., Sathish Kumar, R., Selvarajan, R., Reddy, K., Thomas, G. V., Sasikala, M., Rajeev, G., & Baranwal, V. K. (2010). Molecular detection of 16SrXI group phytoplasma associated with root (wilt) disease of coconut (Cocos nucifera) in India. Plant Disease, 94(5), 636.
Misra, S., & Joshi, H. K. (1983). Light microscopic localization of mycoplasma like organisms in phyllody disease of Pedalium murex L. In H. C. Arya (Ed.), Symposium proceedings (pp. 281). National Symposium on Advancing Frontiers of Plant Sciences, Jodhpur, November 26–30, 1983.
Rajashekar, V., Upender Rao, E., & Srinivas, P. (2012). Biological activities and medicinal properties of Gokhru (Pedalium murex L.). Asian Pacific Journal of Tropical Biomedicine, 2(7), 581–585.
Schneider, B., Seemüller, E., Smart, C. D., & Kirkpatrick, B. C. (1995). Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In S. Razin & J. G. Tully (Eds.), Molecular and Diagnostic Procedures in Mycoplasmology, Vol. I (pp. 369–380). San Diego, CA, USA: Academic Press.
Solomon, J. J., & Geetha, L. (2004). Phytoplasma diseases of coconut in India- root (wilt) and tatipaka diseases. CORD, 20(1), 21–35.
Zhao, Y., Wei, W., Lee, I. M., Shao, J., Suo, X., & Davis, R. E. (2009). Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology, 59, 2582–2593.
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Babu, M., Josephrajkumar, A., Rajumon, M. et al. Molecular characterization of phytoplasma associated with phyllody of Pedalium murex - a common weed in coconut plantations. Phytoparasitica 43, 365–368 (2015). https://doi.org/10.1007/s12600-014-0450-1
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DOI: https://doi.org/10.1007/s12600-014-0450-1