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Phytoparasitica

, Volume 43, Issue 4, pp 471–483 | Cite as

Comparison of analytical techniques used to identify tomato-pathogenic strains of Fusarium oxysporum

  • Amalia Boix-Ruíz
  • Laura Gálvez-Patón
  • Miguel de Cara-GarcíaEmail author
  • Daniel Palmero-Llamas
  • Francisco Camacho-Ferre
  • Julio César Tello-Marquina
Article

Abstract

Two Fusarium oxysporum f. sp. lycopersici (Fol race 2 and Fol race 3) and two f. sp. radicis-lycopersici (Forl) isolates plus eight to ten monosporic descendants from each were identified through molecular analysis and pathogenicity tests using differential varieties under different thermal conditions. The results for molecular and pathogenicity test for Fol were variable and to some extent contradictory. The Fol race 3 isolate and its progeny were amplified by the primers SIX1 and SIX3b but, depending on the test temperatures, the parental plus two descendants lost virulence, and one isolate completely lost virulence in all the tests. The Fol race 2 isolate was amplified by the SIX1 primer, but two descendants were not. The monosporic isolates and the parentals did not show pathogenicity on plants. All Forl isolates and progenies were amplified by the Forl primer, but one of the parentals plus all of its descendants did not show pathogenicity on plants. In addition, temperatures influenced the pathogenicity tests, yielding different virulences for Fol, which was enhanced at higher temperatures, whereas on the contrary a lower temperature was preferable for Forl.

Keywords

Crown and root rot Diagnosis Forma specialis Molecular identification Wilt 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Amalia Boix-Ruíz
    • 1
  • Laura Gálvez-Patón
    • 2
  • Miguel de Cara-García
    • 3
    Email author
  • Daniel Palmero-Llamas
    • 2
  • Francisco Camacho-Ferre
    • 1
  • Julio César Tello-Marquina
    • 1
  1. 1.Grupo de Investigación AGR-200 “Producción Vegetal en Sistemas de Cultivo Mediterráneos”Universidad de AlmeríaAlmeríaSpain
  2. 2.Universidad Politécnica de Madrid. E.U.I.T.A.MadridSpain
  3. 3.Crop ProtectionIFAPA-La MojoneraLa MojoneraSpain

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