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Association of Papaya leaf curl virus with the leaf curl disease of grain amaranth (Amaranthus cruentus L.) in India

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Abstract

A severe leaf curl disease was observed on ~45% of the grain amaranth (Amaranthus cruentus L.) plants growing in experimental plots of CSIR-NBRI, Lucknow, India, during the year 2011. The causal pathogen was transmitted through whiteflies (Bemisia tabaci) from infected to healthy A. cruentus seedlings. Association of Papaya leaf curl virus (PLCV) with the disease was investigated by sequence analysis of ~2.7 kb cloned genome, amplified by Rolling Circle Amplification method from the total DNA of a representative infected A. cruentus sample. The analysis of nucleotide sequence data (accession no. JN135233) revealed 92–94% sequence identities and close phylogenetic relationships with PLCV strains reported from India and Pakistan; hence, the virus isolate of A. cruentus was identified as PLCV. Association of PLCV with leaf curl disease of A. cruentus is being reported for the first time from India.

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References

  • Abou-Jawdah, Y., Maalou, F. R., Shebaro, W., & Soubra, K. (1999). Comparison of the reaction of tomato lines to infection by Tomato yellow leaf curl begomovirus in Lebanon. Plant Pathology, 48, 727–734.

    Article  Google Scholar 

  • Bhatia, A. L. (2005). Growing colorful and nutritious Amaranths. Indian Journal of Natural Products & Resources, 4, 40–43.

    Google Scholar 

  • Briddon, R. W., Bull, S. E., Amin, I., Idris, A. M., Mansoor, S., Bedford, I. D., et al. (2003). Diversity of DNA beta, a satellite molecule associated with some monopartite begomoviruses. Virology, 312, 106–121.

    Article  CAS  PubMed  Google Scholar 

  • Brunt, A. A., Crabtree, K., & Gibbs, A. (1990). Viruses of tropical plants. Wallingford, Oxon, UK: CAB International.

    Google Scholar 

  • Fauquet, C. M., Briddon, R. W., Brown, J. K., Moriones, E., Stanley, J., Zerbini, M., et al. (2008). Geminivirus strain demarcation and nomenclature. Archives of Virology, 153, 783–821.

    Article  CAS  PubMed  Google Scholar 

  • George, B., Vinoth, K. R., & Chakraborty, S. (2013). Molecular characterization of Chilli leaf curl virus and satellite molecules associated with leaf curl disease of Amaranthus spp. Virus Genes, 48, 397–401.

    Article  PubMed  Google Scholar 

  • Horvath, J. (1991). Amaranthus species (Family Amaranthaceae) as host of plant viruses: a review. Acta Phytopathologica et Entomologica Hungarica, 26, 385–422.

    Google Scholar 

  • Ilyas, M., Qazi, J., Mansoor, S., & Briddon, R. W. (2010). Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan. Journal of General Virology, 91, 2091–2101.

    Article  CAS  PubMed  Google Scholar 

  • Lovisolo, O., & Lisa, V. (1976). Studies on Amaranthus leaf mottle virus (ALMV) in the Mediterranean region. Phytopathologia Mediterranea, 27, 89–93.

    Google Scholar 

  • Mansoor, S., Briddon, R. W., Bull, S. E., Bedford, I. D., Bashir, A., Hussain, M., et al. (2003). Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA beta. Archives of Virology, 148, 1969–1986.

    Article  CAS  PubMed  Google Scholar 

  • Morgenstern, B., Frech, K., Dress, A., & Werner, T. (1998). DIALIGN: Finding local similarities by multiple sequence alignment. Bioinformatics, 14, 290–294.

    Article  CAS  PubMed  Google Scholar 

  • Nadeem, A., Mehmood, T., Tahir, M., Khalid, S., & Xing, Z. (1997). First report of papaya leaf curl disease in Pakistan. Plant Disease, 81, 1333.

    Article  Google Scholar 

  • Raj, S. K., Snehi, S. K., Khan, M. S., Chandra, P., & Pandey, R. M. (2008). First molecular identification of a begomovirus associated with yellow vein net disease in grain amaranth (Amaranthus cruentus L.) in India. Australasian Plant Disease Notes, 3, 129–131.

    Article  CAS  Google Scholar 

  • Saxena, S., Hallan, V., Singh, B. P., & Sane, P. V. (1998). Nucleotide sequence and intergeminiviral homologies of the DNA-A of papaya leaf curl geminivirus from India. Biochemistry and Molecular Biology International, 45, 101–113.

    CAS  PubMed  Google Scholar 

  • Segundo, E., Lesemann, D. E., Martin, G., Carmona, M. P., & Ruiz, L. (2007). Amaranthus leaf mottle virus: 3’ end RNA sequence proves classification as distinct virus and reveals affinities within the genus Potyvirus. European Journal of Plant Pathology, 117, 81–87.

    Article  CAS  Google Scholar 

  • Srivastava, A., & Raj, S. K. (2004). High molecular similarity among Indian isolates of Cucumber mosaic virus suggests a common origin. Current Science, 87, 1126–1131.

    CAS  Google Scholar 

  • Srivastava, A., Raj, S. K., Kumar, S., Snehi, S. K., Kulshreshtha, A., Hallan, V., et al. (2013). Molecular identification of Ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased Amaranthus cruentus in India. Virus Genes, 47, 584–590.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

The authors are thankful to the Director of the CSIR-National Botanical Research Institute, Lucknow, for providing the facilities.

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Correspondence to S. K. Raj.

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Srivastava, A., Jaidi, M., Kumar, S. et al. Association of Papaya leaf curl virus with the leaf curl disease of grain amaranth (Amaranthus cruentus L.) in India. Phytoparasitica 43, 97–101 (2015). https://doi.org/10.1007/s12600-014-0424-3

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  • DOI: https://doi.org/10.1007/s12600-014-0424-3

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