Abstract
Citrus yellow mosaic badnavirus (CMBV) is a non-enveloped, bacilliform DNA virus and the etiologic agent of yellow mosaic disease of citrus in India. The disease was initially reported from the southern parts of India and has now spread to other parts of the country. It is a serious disease of sweet orange (Citrus sinensis) in southern India, where it causes significant yield losses. During a recent survey of citrus groves in the Nagpur region, central India, characteristic mosaic symptoms were observed in mandarin orange (Citrus reticulata) and sweet orange. Virus transmission studies, electron microscopy, PCR amplification and sequencing of cloned PCR products from samples showing mosaic symptoms confirmed the presence of a badnavirus. The CMBV–Nagpur isolate could be transmitted to the Rangpur lime (C. limonia) and acid lime (Citrus aurantifolia) by graft inoculation. Sequence analysis of a segment of ORF-III region and intergenic region (IR) of the viral genome revealed that CMBV–Nagpur isolate formed a distinct clade along with some previously reported isolates that are known to infect acid lime and Rangpur lime. CMBV isolates that infect citrus species other than the acid lime and Rangpur lime formed a second clade. Based on the transmission studies and phylogenetic analyses, it was concluded that at least two strains of CMBV exist in India currently.
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References
Ahlawat, Y. S., Chenulu, V. V., Vishwanath, S. M., & Pandey, P. K. (1985). Studies on a mosaic disease of citrus. Current Science, 54, 873–874.
Ahlawat, Y. S., Pant, R. P., Lockhart, B. E., Srivastava, M., Chakraborty, N. K., & Varma, A. (1996a). Association of badnavirus with citrus mosaic disease in India. Plant Disease, 80, 590–592.
Ahlawat, Y. S., Pant, R. P., Shukla, A., & Lockhart, B. E. (1996b). Partial characterization of a badnavirus associated with Citrus yellow mosaic in India. In Proceedings of 13th Conference of the International Organization of Citrus Virologists (China; pp. 208-217).
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.
Anisimova, M., Gil, M., Dufayard, J. F., Dessimoz, C., & Gascuel, O. (2011). Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihood-based approximation schemes. Systematic Biology, 60, 685–699.
Baranwal, V. K., Majumder, S., Ahlawat, Y. S., & Singh, R. P. (2003). Sodium sulphite yields improved DNA of higher stability for PCR detection of Citrus yellow mosaic virus from citrus leaves. Journal of Virological Methods, 112, 153–156.
Baranwal, V. K., Singh, J., Ahlawat, Y. S., Gopal, K., & Charaya, M. U. (2005). Citrus yellow mosaic virus is associated with mosaic disease in Rangpur lime rootstock of citrus. Current Science, 89, 1596–1599.
Borah, B. K., Johnson, A. M., Sai Gopal, D. V., & Dasgupta, I. (2009). Sequencing and computational analysis of complete genome sequences of Citrus yellow mosaic badna virus from acid lime and pummelo. Virus Genes, 39, 137–140.
Bouhida, M., Lockhart, B. E., & Olszewski, N. E. (1993). An analysis of the complete sequence of a sugarcane bacilliform virus genome infectious to banana and rice. Journal of General Virology, 74(Pt 1), 15–22.
Chung, K. R., & Briansky, R. H. (2012). Citrus diseases exotic to Florida: Citrus Yellow Mosaic. EDIS:#PP293.
Dakshinamurti, V., & Reddy, G. S. (1975). Mosaic - a transmissible disorder of sweet oranges. Indian Phytopathology, 28, 398–399.
Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.
EFSA Panel on Plant Health, P. (2008). Scientific opinion of the panel on plant health on a request from the European commission on pest risk assessment made by France on Citrus yellow mosaic virus or Citrus mosaic badnavirus considered by France as harmful in the French overseas departments of French Guiana, Guadeloupe, Martinique and Réunion. The EFSA Journal, 686, 1–16.
Futterer, J., Rothnie, H. M., Hohn, T., & Potrykus, I. (1997). Rice tungro bacilliform virus open reading frames II and III are translated from polycistronic pregenomic RNA by leaky scanning. Journal of Virology, 71, 7984–7989.
Ghosh, D. K., Aglave, B., Bhanare, K., & Baranwal, V. K. (2007). PCR based detection of Citrus yellow mosaic disease from Vidarbha region of Maharastra. Indian Phytopathology, 60, 520–526.
Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W., & Gascuel, O. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321.
Guindon, S., & Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704.
Gupta, K. N., Baranwal, V. K., Prasanna, B. K., Singh, J., Haq, Q. M. R., & Gopal, K. (2009). Genome sequencing, comparison and phylogenetic analysis of Citrus yellow mosaic virus isolates originating from different citrus species in India. Journal of Virology, 5, 143–153.
Hasegawa, M., Kishino, H., & Yano, T. (1985). Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22, 160–174.
Huang, Q., & Hartung, J. S. (2001). Cloning and sequence analysis of an infectious clone of Citrus yellow mosaic virus that can infect sweet orange via Agrobacterium-mediated inoculation. Journal of General Virology, 82, 2549–2558.
Johnson, A. M., Borah, B. K., Sai Gopal, D. V., & Dasgupta, I. (2012). Analysis of full-length sequences of two Citrus yellow mosaic badnavirus isolates infecting Citrus jambhiri (Rough Lemon) and Citrus sinensis L. Osbeck (Sweet Orange) from a nursery in India. Virus Genes, 45, 600–605.
Jones, D. T., Taylor, W. R., & Thornton, J. M. (1992). The rapid generation of mutation data matrices from protein sequences. Computational Applied Biosciences, 8, 275–282.
Peter, P. K., & Chandramohan, R. (2011). Occurrence and distribution of cocoa (Theobroma cocoa L.) diseases in India. Journal of Research ANGRAU, 39, 44–50.
Sai Gopal, D. V. R., Sreenivasulu, M., Raghavendra Rao, G., Venkata Prasanna, T. M., & Subbaiah, K. V. (2000). Characterization and identification of a citrus mosaic badnavirus infecting acid lime (Citrus aurantifolia) in Andhra Pradesh. In Proceedings of the International Symposium on Citriculture (HI TECH Citrus Management, Nagpur, India; pp. 845–852).
Velten, J., Morey, K. J., & Cazzonelli, C. I. (2005). Plant viral intergenic DNA sequence repeats with transcription enhancing activity. Virology Journal, 2, 16.
Waterhouse, A. M., Procter, J. B., Martin, D. M., Clamp, M., & Barton, G. J. (2009). Jalview Version 2–a multiple sequence alignment editor and analysis workbench. Bioinformatics, 25, 1189–1191.
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Ghosh, D.K., Bhose, S., Mukherjee, K. et al. Molecular characterization of Citrus yellow mosaic badnavirus (CMBV) isolates revealed the presence of two distinct strains infecting citrus in India. Phytoparasitica 42, 681–689 (2014). https://doi.org/10.1007/s12600-014-0409-2
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DOI: https://doi.org/10.1007/s12600-014-0409-2