Phytoparasitica

, Volume 42, Issue 3, pp 381–386 | Cite as

Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts

  • Amalendu Ghosh
  • Amrita Das
  • K. B. Pun
  • R. Kumar
  • Ramu Meena
  • V. K. Baranwal
Article

Abstract

Darjeeling hills of West Bengal (India) are well known for the production of mandarin orange (Citrus reticulata). The spread of Citrus tristeza virus (CTV) in this area has threatened the cultivation of citrus. Therefore, a study of the incidence and distribution of CTV in Darjeeling hills was undertaken and a geographical map was prepared. In DAS-ELISA, a high titre of CTV was recorded in the tender shoot bark, leaf petiole and mid-rib of seven Citrus spp. Three citrus species, viz., pomelo, trifoliate and kumquat, were found to be free of CTV infection when tested in double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR).

Keywords

Citrus reticulata Distribution India Mandarin orange Tristeza CTV Darjeeling 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Amalendu Ghosh
    • 1
  • Amrita Das
    • 1
  • K. B. Pun
    • 1
    • 3
  • R. Kumar
    • 1
    • 4
  • Ramu Meena
    • 1
  • V. K. Baranwal
    • 2
  1. 1.Indian Agricultural Research Institute, Regional StationKalimpongIndia
  2. 2.Advanced Centre for Plant VirologyIndian Agricultural Research InstituteNew DelhiIndia
  3. 3.Regional Rainfed Lowland Rice Research Station, Central Rice Research InstituteGeruaIndia
  4. 4.Indian Agricultural Research Institute, Regional StationKarnalIndia

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