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Pathogenic and coat protein characterization confirming the occurrence of Bean common mosaic virus on common bean (Phaseolus vulgaris) in Kashmir, India

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Abstract

Bean common mosaic virus (BCMV), belonging to the family Potyviridae, is a serious pathogen of common bean (Phaseolus vulgaris L.) causing considerable economic losses owing to seed, sap and aphid transmissibility. The viral nature of the test isolates and identity of the virus as BCMV were confirmed by mechanical transmission and DAS-ELISA using BCMV antiserum. Pathogenic variability studies in BCMV infecting common bean (Phaseolus vulgaris L.) in Jammu and Kashmir (a northwestern Himalayan state of India), revealed the existence of three pathogroups – PG-I, PG-II and PG-VII, accommodating five strains (NL-1, NL-1n, NL-4, NL-7 and NL-7n). Comparative sequence analysis of coat protein gene revealed that the strains NL-1, NL-4 and NL-7 shared more than 90% amino acid sequence homology with other BCMV isolates from other countries. DAG motif as well as BCMV specific conserved motif MVWCIDN were present in all the three strains. Phylogenetic analysis of coat protein also clustered them in the BCMV group. This study confirmed the occurrence of BCMV and its strains on common bean in Kashmir.

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Authors and Affiliations

  1. Division of Plant Pathology, SKUAST- Kashmir, Shalimar, Srinagar, 191121, Jammu & Kashmir, India

    Aflaq Hamid, Mushtaq Ahmad, B. A. Padder, M. D. Shah, Sehar Saleem, T. A. Sofi & A. A. Mir

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  1. Aflaq Hamid
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  2. Mushtaq Ahmad
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  3. B. A. Padder
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  4. M. D. Shah
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  5. Sehar Saleem
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  6. T. A. Sofi
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  7. A. A. Mir
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Correspondence to Aflaq Hamid.

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Supplementary Fig. 1

Symptoms of Bean common mosaic virus isolates on susceptible cv. SR1 (a: mosaic, b: blistering and green vein banding, c: downward rolling) and Jubila (e and f: necrotic local lesions and veinal necrosis induced at temperature >30°C) (DOC 983 kb)

Supplementary Fig. 2

Phylogenetic tree showing amino acid relationship of Kashmir strains with other BCMV strains at coat protein (CP only) (DOC 176 kb)

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Hamid, A., Ahmad, M., Padder, B.A. et al. Pathogenic and coat protein characterization confirming the occurrence of Bean common mosaic virus on common bean (Phaseolus vulgaris) in Kashmir, India. Phytoparasitica 42, 317–322 (2014). https://doi.org/10.1007/s12600-013-0362-5

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  • DOI: https://doi.org/10.1007/s12600-013-0362-5

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