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Possible correlations between the characteristics of Potato leafroll virus isolates occurring in different geographical regions in Tunisia

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Abstract

Biological and molecular characterization supported by transmission efficiency, symptom expression and Open Reading Frame 0 (ORF0) nucleotide sequence analysis were carried out to assess nine isolates of Potato leafroll virus (PLRV) collected from three Tunisian geographic and bioclimatic zones. Plant-to-plant transmission by Tunisian Myzus persicae aphid clones showed high transmission efficiency for all isolates tested. Symptom expression analysis on a Physalis floridana plant test distinguished viral isolates as very severe, severe and mild. The ORF0 sequences of the Tunisian PLRV isolates showed an assignment to two aggregates when compared with GenBank PLRV sequences. A significant correlation between symptom severity and ORF0 nucleotide sequence or between symptom severity and geographic origins of the PLRV isolates was established. However, the transmission efficiency and the ORF0 sequence were not affected by the bioclimatic origin. No significant correlation between transmission and symptom or between transmission and the ORF0 sequence was detected.

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Acknowledgments

This work was supported by grants from the Ministry of High Education, the Ministry of Scientific Research Technology and Competence development, the CNRS of France, and the French Embassy in Tunisia. We thank Mr. Nouri Khamassy, who kindly supplied us with some PLRV isolates. We are grateful to Mr. Bernard Tivoli and Mr. Maurice Hulle for allowing us to progress with this work in the UMR BiO3P of the National Institute of Agronomic Research in Rennes (France). We are indebted to Danièle Giblot-Ducray for critical comments during this work and for providing all molecular materials.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis (FST), Campus Universitaire El Manar, 2092, Tunis, Tunisia

    Fattouma Djilani Khouadja & Hatem Fakhfakh

  2. Institut Supérieur de Biotechnologie de Sidi Thabet (ISBST), Sidi Thabet, Tunisia

    Fattouma Djilani Khouadja

  3. Unité de Recherche INRA/ENSAR Biologie des Organismes et des Populations Appliquées à la Protection des Plantes (UMR BiO3P), INRA Le Rheu, Domaine de la Motte, BP 35327-35653, Rennes Cedex, France

    Joelle Rouzé-Jouan

  4. INRA Science and Impact, UR 1321 Agrosystèmes tropicaux, Domaine Duclos-Prise d’Eau, Petit-Bourg, F-97170, France

    Sébastien Guyader

  5. Faculté des Sciences de Bizerte (FSB), Bizerte, Tunisia

    Hatem Fakhfakh

Authors
  1. Fattouma Djilani Khouadja
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  2. Joelle Rouzé-Jouan
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  3. Sébastien Guyader
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  4. Hatem Fakhfakh
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Correspondence to Fattouma Djilani Khouadja.

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Khouadja, F.D., Rouzé-Jouan, J., Guyader, S. et al. Possible correlations between the characteristics of Potato leafroll virus isolates occurring in different geographical regions in Tunisia. Phytoparasitica 42, 259–267 (2014). https://doi.org/10.1007/s12600-013-0329-6

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  • DOI: https://doi.org/10.1007/s12600-013-0329-6

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