Abstract
Infectivity of Spodoptera litura multiple nucleopolyhedrovirus (SpltMNPV) was compared between before (P0) and after (P1–P4) passage in subsequent generations through the gut of Eocanthecona furcellata. Viable virus was detected in E. furcellata feces up to 6 days after feeding on infected S. litura larvae. NPV mortality ranged between 93% and 10% when test larvae were exposed to polyhedra voided in feces collected after 1 and 6 days post-infected meal, respectively. The mean number of polyhedral occlusion bodies (POBs) in excreta and their infectivity (%) at all passages did not vary significantly. The comparison of observed LD50 and ST50 values among all passages did not reveal significant differences owing to their overlapping confidence limits. The gut-passed virus did not show a detrimental effect on survival rate, longevity, fecundity and percent egg hatchability of E. furcellata in the subsequent three generations. A field trial was also conducted to estimate virus dissemination through feces of predators that were fed upon prey infected with polyhedra before passage, after passage and healthy (control) prey and subsequently released on cabbage plants. An additional viral mortality up to the magnitude of 13–17% was noticed in the former two treatments. However, within these two treatments the viral mortality did not vary significantly. It was concluded that E. furcellata disseminated the virus through their feces into the ecosystem without any adverse effect to it and infectivity of the SpltMNPV is not altered after passage through the gut of the predator.
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Acknowledgments
The authors are grateful to an anonymous reviewer for suggestions for improvement of the manuscript, and to Dr. M. Nagata for guidance during the course of the investigation. Our sincere thanks are also due to the Department of Science and Technology, Government of India, for funding and the National Bureau of Agriculturally Important Insects (NBAII) for encouragement and co-operation.
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Gani, M., Gupta, R.K., Kaul, V. et al. Effect of passage through the gut of Eocanthecona furcellata on Spodoptera litura multiple nucleopolyhedrovirus infectivity and its subsequent dissemination. Phytoparasitica 41, 327–335 (2013). https://doi.org/10.1007/s12600-013-0293-1
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DOI: https://doi.org/10.1007/s12600-013-0293-1