Isolation and characterization of gut bacteria of fruit fly, Bactrocera tau (Walker)

Phytoparasitica volume 41, pages193–201(2013)Cite this article

Abstract

Gut bacteria of fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae), were isolated and the isolates attractive to B. tau adults were characterized using morphological, biochemical and 16S rRNA analyses to determine their taxonomic position. Based upon morphological, biochemical and 16S rRNA sequences (on the basis of closest match), five gut bacterial species of B. tau were characterized as Delftia acidovorans, Pseudomonas putida, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp., of which four bacterial isolates, viz., Delftia acidovorans, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp. are new records from guts of the fruit fly species.

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Acknowledgments

The authors are grateful to three anonymous reviewers for their helpful critical comments on the manuscript. They also thank Miss Kajal (Senior Research Fellow, Molecular Plant Pathology Laboratory, CSKHPKV, Palampur) for the help rendered during the study.

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Affiliations

  1. Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India

    Chandra S. Prabhakar, Pankaj Sood & Pawan K. Mehta

  2. Department of Microbiology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India

    Sarbjit S. Kanwar & Ajay Kumar

  3. Molecular Plant Pathology Laboratory, Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India

    Prem N. Sharma

  4. International Crops Research Institute for the Semi Arid Tropics, Patancheru, Andhra Pradesh, 502 324, India

    Chandra S. Prabhakar

  5. Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh, 175 019, India

    Pankaj Sood

Authors
  1. Chandra S. Prabhakar
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  2. Pankaj Sood
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Correspondence to Chandra S. Prabhakar.

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Prabhakar, C.S., Sood, P., Kanwar, S.S. et al. Isolation and characterization of gut bacteria of fruit fly, Bactrocera tau (Walker). Phytoparasitica 41, 193–201 (2013). https://doi.org/10.1007/s12600-012-0278-5

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Keywords

  • Defluvibacter
  • Delftia
  • Flavobacterium
  • Ochrobactrum
  • Phylogeny
  • Pseudomonas
  • 16S rRNA