, Volume 39, Issue 3, pp 269–283 | Cite as

Biochemical and molecular studies of early blight disease in tomato

  • Suchita J. PatelEmail author
  • R. B. Subramanian
  • Yachana S. Jha


Tomato early blight occurs worldwide and it is prevalent wherever tomatoes are grown. Alternaria solani Sorauer, the causal agent, has been recognized as a serious foliar pathogen of tomato and there are very few cultivars which possess resistance against early blight. Alternaric acid is the major toxin of A. solani. In this study, alternaric acid and fungal culture filtrate were used as an elicitor in NDT-96 (tolerant) and GP-5 (susceptible) tomato varieties in order to study and compare their abilities to induce defense-related enzymes, viz., catalase, peroxidase, β-1,3 glucanase, phenylalanine-ammonia-lyase (PAL), chitinase and polyphenol-oxidase (PPO) along with total phenols, and total soluble proteins. NDT-96 showed a rapid induction of all these pathogenesis-related enzymes except catalase along with total phenols as compared to GP-5 with both the treatments. Differential expression of total soluble proteins revealed higher protein content in NDT-96 as compared with GP-5. A 49.48 kDa protein was observed to be absent in GP-5. In addition, 25 microsatellite markers (SSR) were screened for polymorphisms among the above mentioned two tomato varieties. Of these, SSR 286 revealed a significant polymorphic band of 108 bp in NDT-96.


Alternaria solani Pathogenesis-related enzymes Simple sequence repeats Systemic acquired resistance 



We are very thankful to Dr. Subhash Patel, Anand Agricultural University, India, for providing the Alternaria solani isolate.


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Copyright information

© Springer Science & Business Media BV 2011

Authors and Affiliations

  • Suchita J. Patel
    • 1
    Email author
  • R. B. Subramanian
    • 1
  • Yachana S. Jha
    • 1
  1. 1.B. R. Doshi School of Biosciences, Satellite CampusSardar Patel UniversityVallabh VidhyanagarIndia

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