Abstract
The current study is based on the screening of novel insecticides from new sources that have not being exploited hitherto. The major objectives of this research work were to extract marine molluscs, Lambis lambis, Trochus radiatus and Chicoreus ramosus, from Tuticorin coastal waters using different solvents to test their insecticidal properties and partially purify the active components. The ethyl acetate extracts of L. lambis and T. radiatus showed 100% mortality of Sitophilus oryzae, at a concentration of 200 μg ml−1. The LC50 values for ethyl acetate extracts of L. lambis, T. radiatus and C. ramosus were found to be 67.08, 348.86 and 571.42 μg ml−1, respectively. With regard to bacterial metabolites, at a concentration of 200 μg ml−1 the ethyl acetate extract of A3 was able to elicit an activity of 40% and that of strain A1 – 20%. The LC50 values of the bacterial metabolites were also investigated. Upon chromatographic separation of active ethyl acetate extracts of T. radiatus, the 100% methanol column-purified fraction was found to have an activity of 30% at a concentration of 10 μg against S. oryzae. The purity of the partially pure active compound was observed to be 72.78% on analysis with high pressure thin layer chromatography.
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Acknowledgments
We are thankful to Suganthi Devadason Marine Research Institute (SDMRI), Tuticorin, Tamil Nadu, for providing mollusc samples. We also acknowledge the Technical assistance provided by the Central Research Facility (CFR), Sri Ramachandra University, Porur, Chennai, Tamil Nadu, in analyzing the samples via HPTLC.
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Gnanambal, M.E.K., Devi, K.C.R., Chandra, P.H. et al. High Performance Thin Layer Chromatography (HPTLC) profile of an insecticidal compound of Trochus radiatus and assessment of mucus-associated bacterial extracts. Phytoparasitica 39, 121–127 (2011). https://doi.org/10.1007/s12600-010-0140-6
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DOI: https://doi.org/10.1007/s12600-010-0140-6