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Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica)

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Abstract

Apple mosaic virus (ApMV) is an important virus of apple worldwide. Surveys were conducted in the states of Himachal Pradesh (HP) and Jammu & Kashmir (J&K), India, to determine the prevalence of ApMV and to develop ways for its early diagnosis. Symptomatic leaf samples, bud and bark were collected during spring and late summer. DAS-ELISA revealed the presence of ApMV in 13/198 samples from four districts in HP, indicating a disease incidence of approximately 6.5%. The sequence of amplicons produced by reverse transcription-polymerase chain reaction (RT-PCR) using virus-specific primers confirmed the ELISA results. Comparison of the sequences of three amplicons with nine complete available sequences of ApMV coat protein (CP) (from apple) at amino acid level revealed a maximum of 96% homology to a Korean isolate of ApMV (AY125977). Comparison of the amino acid sequence of the CP of the Indian isolate with the amino acid sequence of the CP from different hosts showed that the Indian isolate clustered most closely with an isolate found in pear and originating from Czechoslovakia. Cloned DNA was reliably used for diagnosis of the virus and was a useful tool for screening at nursery level using slot blot hybridization. This study confirms the presence of ApMV at the molecular level in India and reveals sequence information.

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Acknowledgments

The authors express their gratitude to the Director, Institute of Himalayan Bioresource Technology, Palampur (HP), India, for encouragement and providing the necessary facilities; and to the Council of Scientific and Industrial Research for Supra Institutional Project for grant SIP 003.

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Authors and Affiliations

  1. Plant Virus Lab, Institute of Himalayan Bioresource Technology, Palampur, 176061, H.P., India

    Tombisana Thokchom, Tanuja Rana, Vipin Hallan, Raja Ram & Aijaz A. Zaidi

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  1. Tombisana Thokchom
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  2. Tanuja Rana
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  3. Vipin Hallan
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  4. Raja Ram
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  5. Aijaz A. Zaidi
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Correspondence to Vipin Hallan.

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Thokchom, T., Rana, T., Hallan, V. et al. Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica). Phytoparasitica 37, 375–379 (2009). https://doi.org/10.1007/s12600-009-0041-8

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  • DOI: https://doi.org/10.1007/s12600-009-0041-8

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