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Protection of Arsenic Induced Oxidative Stress in Reproductive Organs and Female Infertility by Pectic Polysaccharide of Momordica charantia: An In Vivo Study in dose Dependent Manner

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Abstract

Arsenic is a serious environmental pollutant and reprotoxic agent. Pectic Polysaccharide (CCPS) from Momordica charantia is an antioxidant used to treat various disorders. This study aimed to elucidate the effect of different and effective doses of CCPS on the female reproductive system against arsenic-induced toxicity by co-administration mode. Thirty rats were equally distributed into five groups. Designated as Control, AsIII+ (10.0 mg/Kg Body-Weight) and Co-treatment of CCPS in different doses (1.5 mg/Kg BW), CCPS (2.0 mg/Kg BW), CCPS (2.5 mg/Kg BW). Rats were administered their respective doses orally every day for eight days. Here, the most effective doses at CCPS (2.0 mg/Kg BW) and CCPS (2.5 mg/Kg BW) significantly improved AsIII+ induced uterine lipid peroxidation state and ROS generation by the re-establishment of Superoxide Dismutase and catalase activities. In addition, these doses (2.0 mg and 2.5 mg) at CCPS improved ovarian steroidogenesis activities and restored uterine-arsenic-treated rats by controlling LH, FSH, and estradiol levels. The present study revealed that Isolated CCPS obtained the D-galactose and D-methyl galacturonate components (1:4), and the IR spectrum indicated a possible chelating property of CCPS with the presence of binding sites (OH–/COOH) for arsenic. So, We conclude that galacturonate acid residue in doses at CCPS (2.0 mg/Kg BW) and CCPS (2.5 mg/Kg BW) CCPS may contribute a potential interaction with sodium arsenite and play a critical role against arsenic-mediated uterine and ovarian toxicity by the possible regulation of S-adenosine methionine (SAM) pool.

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References

  • Agrawal, S., G. Flora, P. Bhatnagar, and S.J.S. Flora. 2014. Comparative oxidative stress, metallothionein induction and organ toxicity following chronic exposure to arsenic, lead and mercury in rats. Cellular and Molecular Biology 60 (2): 13–21.

    CAS  PubMed  Google Scholar 

  • Ahmad, S.A., M.H. Sayed, S. Barua, M.H. Khan, M.H. Faruquee, A. Jalil, S.A. Hadi, and H.K. Talukder. 2001. Arsenic in drinking water and pregnancy outcomes. Environmental Health Perspectives 109 (6): 629–631.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Akila, P., and L. Vennila. 2016. Chlorogenic acid a dietary polyphenol attenuates isoproterenol induced myocardial oxidative stress in rat myocardium: An in vivo study. Biomedicine & Pharmacotherapy 84: 208–214.

    Article  CAS  Google Scholar 

  • Aydin, G., K.A.Y.A. Ertuğrul, and A. Review. 2020. Momordica charantia L.‘s biological active components and its potential use in traditional therapies. International Journal of Traditional and Complementary Medicine Research 1 (2): 79–95.

    Google Scholar 

  • Bhattacharya, S., and P.K. Haldar. 2013. The triterpenoid fraction from Trichosanthes dioica root suppresses experimentally induced inflammatory ascites in rats. Pharmaceutical Biology 51 (11): 1477–1479.

    Article  CAS  PubMed  Google Scholar 

  • Budrat, P., and A. Shotipruk. 2008. Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts. Chiang Mai Journal of Science 35 (1): 123–130.

    CAS  Google Scholar 

  • Champlin, A.K., D.L. Dorr, and A.H. Gates. 1973. Determining the stage of the estrous cycle in the mouse by the appearance of the vagina. Biology of Reproduction 8 (4): 491–494.

    Article  CAS  PubMed  Google Scholar 

  • Chao, C.Y., and C.J. Huang. 2003. Bitter gourd (Momordica charantia) extract activates peroxisome proliferator–activated receptors and upregulates the expression of the acyl CoA oxidase gene in H4IIEC3 hepatoma cells. Journal of Biomedical Science 10 (6): 782–791.

    PubMed  Google Scholar 

  • Dash, M., M. Maity, A. Dey, H. Perveen, S. Khatun, L. Jana, and S. Chattopadhyay. 2018. The consequence of NAC on sodium arsenite-induced uterine oxidative stress. Toxicology Reports 5: 278–287.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Devasagayam, T.P.A., K.K. Boloor, and T. Ramasarma. 2003. Methods for estimating lipid peroxidation: An analysis of merits and demerits. Indian Journal of Biochemistry & Biophysics 40 (5): 300–308.

    CAS  Google Scholar 

  • Duan, Z.Z., X.L. Zhou, Y.H. Li, F. Zhang, F.Y. Li, and Q. Su-Hua. 2015. Protection of Momordica charantia polysaccharide against intracerebral hemorrhage-induced brain injury through JNK3 signaling pathway. Journal of Receptors and Signal Transduction 35 (6): 523–529.

    Article  CAS  PubMed  Google Scholar 

  • El-Zoghbi, M., and M.Z. Sitohy. 2001. Mineral absorption by albino rats as affected by some types of dietary pectins with different degrees of esterification. Food/Nahrung 45 (2): 114–117.

    Article  CAS  PubMed  Google Scholar 

  • Flora, S.J. 2009. Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxidative Medicine and Cellular Longevity 2 (4): 191–206.

    Article  PubMed  PubMed Central  Google Scholar 

  • Fournier, L., G. Thomas, R. Garnier, A. Buisine, P. Houze, F. Pradier, and S. Dally. 1988. 2,3-Dimercaptosuccinic acid treatment of heavy metal poisoning in humans. Medical Toxicology and Adverse Drug Experience 3 (6): 499–504.

    CAS  PubMed  Google Scholar 

  • Gong, J., F. Sun, Y. Li, X. Zhou, Z. Duan, F. Duan, L. Zhao, H. Chen, S. Qi, and J. Shen. 2015. Momordica charantia polysaccharides could protect against cerebral ischemia/reperfusion injury through inhibiting oxidative stress mediated c-Jun N-terminal kinase 3 signaling pathway. Neuropharmacology 91: 123–134.

    Article  CAS  PubMed  Google Scholar 

  • Grover, J.K., and S.P. Yadav. 2004. Pharmacological actions and potential uses of Momordica Charantia: A review. Journal of Ethnopharmacology 93 (1): 123–312.

    Article  CAS  PubMed  Google Scholar 

  • Hadwan, M.H. 2016. New method for assessment of serum catalase activity. Indian Journal of Science and Technology 9 (4): 1–5.

    Article  CAS  Google Scholar 

  • Hopenhayn, C., C. Ferreccio, S.R. Browning, B. Huang, C. Peralta, H. Gibb, and I. Hertz-Picciotto. 2003. Arsenic exposure from drinking water and birth weight. Epidemiology (Cambridge, Mass.) 14 (5): 593–602.

    Article  PubMed  Google Scholar 

  • Hultberg, B., A. Andersson, and A. Isaksson. 2001. Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitol. Toxicology 156 (2–3): 93–100.

    Article  CAS  PubMed  Google Scholar 

  • Jarabak, J., J.A. Adams, H.G. Williams-Ashman, and P. Talalay. 1962. Purification of a 17β-hydroxysteroid dehydrogenase of human placenta and studies on its transhydrogenase function. Journal of Biological Chemistry 237 (2): 345–357.

    Article  CAS  PubMed  Google Scholar 

  • Kosnett, M.J. 2013. The role of chelation in the treatment of arsenic and mercury poisoning. Journal of Medical Toxicology 9: 347–354.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar, A. 2012. Effect of simuastation on paraxonase 1 (PON1) activity and oxidation stress. Significance of Lipid Profile Assay as Diagnostic and Prognostic Tool Create Space Independent Publishing Platform,105–109.

  • Lewis, A., J. Du, J. Liu, J.M. Ritchie, L.W. Oberley, and J.J. Cullen. 2005. Metastatic progression of pancreatic cancer: Changes in antioxidant enzymes and cell growth. Clinical & Experimental Metastasis 22 (7): 523–532.

    Article  CAS  Google Scholar 

  • Maity, M., H. Perveen, M. Dash, S. Jana, S. Khatun, A. Dey, A.K. Mandal, and S. Chattopadhyay. 2018. Arjunolic acid improves the serum level of vitamin B 12 and folate in the process of the attenuation of arsenic induced uterine oxidative stress. Biological Trace Element Research 182 (1): 78–90.

    Article  CAS  PubMed  Google Scholar 

  • Maji, P.K., I.K. Sen, B. Behera, T.K. Maiti, P. Mallick, and S.R. Sikdar. 2012. Structure characterization and study of immune enhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus Florida and Lentinulaedodes. Carbohydrate Research 358: 110–115.

    Article  CAS  PubMed  Google Scholar 

  • Marshall, G., C. Ferreccio, Y. Yuan, M.N. Bates, C. Steinmaus, S. Selvin, J. Liaw, and A.H. Smith. 2007. Fifty-year study of lung and bladder cancer mortality in Chile related to arsenic in drinking water. Journal of the National Cancer Institute 99 (12): 920–928.

    Article  CAS  PubMed  Google Scholar 

  • Mishra, D., A. Mehta, and S.J. Flora. 2008. Reversal of arsenic-induced hepatic apoptosis with combined administration of DMSA and its analogues in guinea pigs: Role of glutathione and linked enzymes. Chemical Research in Toxicology 21 (2): 400–407.

    Article  CAS  PubMed  Google Scholar 

  • Nie, J.S., Q.L. Pei, G. Han, J.X. Xu, and J.J. Mu. 2006. Semen quality decreased by inorganic arsenic. Journal of Occupational and Environmental Medicine 23: 189–190.

    CAS  Google Scholar 

  • Panda, B.C., S. Mondal, K.S.P. Devi, T.K. Maiti, S. Khatua, K. Acharya, and S.S. Islam. 2015. Pectic polysaccharide from the green fruits of Momordica charantia (Karela): Structural characterization and study of immunoenhancing and antioxidant properties. Carbohydrate Research 401: 24–31.

    Article  CAS  PubMed  Google Scholar 

  • Pant, N., R.C. Murthy, and S.P. Srivastava. 2004. Male reproductive toxicity of sodium arsenite in mice. Human & Experimental Toxicology 23 (8): 399–403.

    Article  CAS  Google Scholar 

  • Pattichis, K., L.L. Louca, and V. Glover. 1994. Quantitation of soluble superoxide dismutase in rat striata, based on the inhibition of nitrite formation from hydroxylammonium chloride. Analytical Biochemistry 221 (2): 428–431.

    Article  CAS  PubMed  Google Scholar 

  • Perveen, H., M. Dash, S. Khatun, M. Maity, S.S. Islam, and S. Chattopadhyay. 2017. Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin. Biochemistry and Biophysics Reports 11: 64–71.

    Article  PubMed  PubMed Central  Google Scholar 

  • Perveen, H., S. Chattopadhyay, M. Maity, M. Dash, and S.S. Islam. 2019. Involvement of proinflammatory cytokines and metallothionein in the repairing of arsenic-mediated uterine tissue damage by curcumin. Journal of Basic and Clinical Physiology and Pharmacology 30 (4): 20170179.

    Article  CAS  Google Scholar 

  • Pi, J., S. Horiguchi, Y. Sun, M. Nikaido, N. Shimojo, T. Hayashi, H. Yamauchi, K. Itoh, M. Yamamoto, G. Sun, and M.P. Waalkes. 2003. A potential mechanism for the impairment of nitric oxide formation caused by prolonged oral exposure to arsenate in rabbits. Free Radical Biology and Medicine 35 (1): 102–113.

    Article  CAS  PubMed  Google Scholar 

  • Raish, M. 2017. Momordica charantia polysaccharides ameliorate oxidative stress, hyperlipidemia, inflammation, and apoptosis during myocardial infarction by inhibiting the NF-κB signaling pathway. International Journal of Biological Macromolecules 97: 544–551.

    Article  CAS  PubMed  Google Scholar 

  • Raish, M., A. Ahmad, B.L. Jan, K.M. Alkharfy, M.A. Ansari, K. Mohsin, F. Al Jenoobi, and A. Al-Mohizea. 2016. Momordica charantia polysaccharides mitigate the progression of STZ induced diabetic nephropathy in rats. International Journal of Biological Macromolecules 91: 394–399.

    Article  CAS  PubMed  Google Scholar 

  • Ratnaike, R.N. 2003. Acute and chronic arsenic toxicity. Postgraduate Medical Journal 79 (933): 391–396.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sharma, A., M.K. Sharma, and M. Kumar. 2009. Modulatory role of Emblica officinalis fruit extract against arsenic induced oxidative stress in Swiss albino mice. Chemico-biological Interactions 180 (1): 20–30.

    Article  CAS  PubMed  Google Scholar 

  • Shi, H., X. Shi, and K.J. Liu. 2004. Oxidative mechanism of arsenic toxicity and carcinogenesis. Molecular and Cellular Biochemistry 255: 67–78.

    Article  CAS  PubMed  Google Scholar 

  • Talalay, P. 1962. Hydroxysteroid dehydrogenase. In Methods in enzymology, ed. S.P. Colowick. New York: Academic Press.

    Google Scholar 

  • Tiwari-Pandey, R., and M. Ram Sairam. 2009. Modulation of ovarian structure and abdominal obesity in curcumin-and flutamide-treated aging FSH-R haploinsufficient mice. Reproductive Sciences 16 (6): 539–550.

    Article  CAS  PubMed  Google Scholar 

  • Uddin, R., and N.H. Huda. 2011. Arsenic poisoning in Bangladesh. Oman Medical Journal 100 (323): 1–3.

    Google Scholar 

  • Usoh, I.F., E.J. Akpan, E.O. Etim, and E.O. Farombi. 2005. Antioxidant actions of dried flower extracts of Hibiscus sabdariffa L. on sodium arsenite-induced oxidative stress in rats. Pakistan Journal of Nutrition 4 (3): 135–141.

    Article  Google Scholar 

  • Wang, Q., X. Wu, F. Shi, and Y. Liu. 2019. Comparison of antidiabetic effects of saponins and polysaccharides from Momordica charantia L. in STZ-induced type 2 diabetic mice. Biomedicine & Pharmacotherapy 109: 744–750.

    Article  CAS  Google Scholar 

  • Wang, H., S. Zhang, M. Guo. L, Irfan, G. Kan, and H. Chen. 2013. Study on correlation between antioxidant and hypoglycemic activity polysaccharide isolation from Momordica charantia. Bothalia Journal 43: 117–128.

    CAS  Google Scholar 

  • Weydert, C.J., and J.J. Cullen. 2010. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nature Protocols 5 (1): 51–66.

    Article  CAS  PubMed  Google Scholar 

  • Zeng, Q., C.H. Ko, W.S. Siu, L.F. Li, X.Q. Han, L. Yang, C. Bik-San Lau, J.M. Hu, and P.C. Leung. 2017. Polysaccharides of Dendrobium officinale Kimura & Migo protect gastric mucosal cell against oxidative damage-induced apoptosis in vitro and in vivo. Journal of Ethnopharmacology 208: 214–224.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, C., H. Chen, and W. Bai. 2018. Characterization of Momordica charantia L. polysaccharide and its protective effect on pancreatic cells injury in STZ-induced diabetic mice. International Journal of Biological Macromolecules 115: 45–52.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Allied Health Science, Brainware University, Barasat, Kolkata, West Bengal, 700125, India

    Hasina Perveen

  2. Department of Biomedical Laboratory Science and Management, Vidyasagar University, Midnapore, West Bengal, 721102, India

    Sandip Chattopadhyay

  3. Clinical Nutrition and Dietetics Division, Vidyasagar University, Midnapore, West Bengal, 721102, India

    Sandip Chattopadhyay

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  1. Hasina Perveen
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Perveen, H., Chattopadhyay, S. Protection of Arsenic Induced Oxidative Stress in Reproductive Organs and Female Infertility by Pectic Polysaccharide of Momordica charantia: An In Vivo Study in dose Dependent Manner. Proc Zool Soc 77, 105–115 (2024). https://doi.org/10.1007/s12595-024-00513-9

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