Abstract
Lophiosilurus alexandri (pacamã) is a native fish of the São Francisco River in Brazil. Importantly, its liver bioenergetic metabolism has never been investigated. As the center of metabolism in any aerobic cell is the mitochondria, we sought to investigate some bioenergetic parameters of hepatic mitochondria from pacamã and compare them to those of Oreochromis niloticus (tilápia). The activity of each respiratory chain complex, H2O2 and membrane potential were evaluated in isolated liver mitochondria. Some hepatic antioxidant enzymes activity and the mitochondrial structure using transmission electron microscopy were also evaluated. A comparison of tilápia and pacamã revealed that the mitochondrial respiratory control was lower in pacamã, for both complex I (20%) and complex II (28%). The mitochondria from pacamã were also sensitive to Ca2+, and produced higher amounts of H2O2 (19%) than were produced in tilápia. Additionally, liver antioxidant enzyme activity levels (catalase and glutathione peroxidase) in pacamã were significantly decreased. However, SOD activity was enhanced. Finally, ultrastructural analysis showed morphological characteristics of stress. In conclusion, liver mitochondria in pacamã seems to have functional respiratory chain complexes, membrane potential Ca2+ sensitive, produces H2O2 and have an active antioxidant enzyme system, these results are in agreement with the ultrastructural evaluation.
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Acknowledgements
The authors are thankful to Dr. Heberty di Tarso F. Facundo for his critical reading of this paper. The authors are also grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), with the Grant Number 484490/2013-3, for the financial support and for the scholarship to JASS, RCS; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship to SKMC and ANM, and also to UFAL for the scholarship to STVM and EJBF.
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SKMC and JASS: Conceptualization, methodology, investigation, writing—original draft, visualization. EJBF and STVM: Conceptualization, methodology, investigation. ÁMN: Methodology, investigation. KLAS and RCBQF: Conceptualization, methodology, investigation, writing—original draft. RCS-F: Methodology, investigation. MPF: Conceptualization, methodology, investigation, writing—original draft, visualization. MBSM: Methodology, writing—review and editing, visualization. ECM: Methodology, writing—review and editing, visualization. ACRL: Investigation, writing—review and editing, writing—original draft, visualization, project administration.
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Cavalcante, S.K.M., da Silva Santos, J.A., Ferreira, E.J.B. et al. Lophiosilurus alexandri Functional Studies Related to Mitochondrial Bioenergetics. Proc Zool Soc 76, 190–201 (2023). https://doi.org/10.1007/s12595-023-00493-2
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DOI: https://doi.org/10.1007/s12595-023-00493-2