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In Vitro Anthelmintic Efficacy of Sesbania sesban var. bicolor, Cyperus compressus and Asparagus racemosus Against Gastrothylax crumenifer (Trematoda)

Abstract

The Santhal tribe in Assam, India utilize Sesbania sesban var. bicolor, Cyperus compressus and Asparagus racemosus to treat intestinal-worm infections. This study reports the in vitro anthelmintic efficacy of these plants against Gastrothylax crumenifer (Trematoda). The test parasites were exposed to 10, 20 and 30 mg/ml concentrations of each plant extract. The paralysis and mortality time of parasites were recorded and compared with a reference anthelmintic drug, praziquantel (PZQ). The paralysed worms were processed for scanning electron microscopy (SEM). All the plant extracts exhibited significant anti-worm activity in a dose-dependent manner and the activities were better than that of PZQ. The highest efficacy was observed for S. sesban var. bicolor, where exposure to 30 mg/ml methanol extract of the leaves caused parasite mortality within 1.02 ± 0.004 h. The SEM studies revealed adverse effects on tegument in the form of depressions around the sucker and acetabulum in parasites that were exposed to S. sesban var. bicolor and C. compressus extracts. Also, the tegument appeared wrinkled in parasites that were exposed to S. sesban var. bicolor and A. racemosus extracts. Collectively, this study showed considerably high trematocidal efficacy of the methanolic leaf extract of S. sesban var. bicolor and provides scientific evidence in support of its use in traditional medicine.

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Acknowledgements

The authors thank Dr. P.B. Gurung, plant taxonomist from the Department of Botany, NEHU for the identification of the plant materials. SEM study was undertaken at AIIMS, New Delhi.

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AKY conceived and designed the study, ADS performed the experiments, analysed the data and wrote the first draft. Draft was finalized by AKY. All authors read and approved the final manuscript.

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Correspondence to Amar Deep Soren.

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Soren, A.D., Yadav, A.K. In Vitro Anthelmintic Efficacy of Sesbania sesban var. bicolor, Cyperus compressus and Asparagus racemosus Against Gastrothylax crumenifer (Trematoda). Proc Zool Soc 74, 262–267 (2021). https://doi.org/10.1007/s12595-021-00370-w

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Keywords

  • Anthelmintic efficacy
  • Asparagus racemosus
  • Cyperus compressus
  • Gastrothylax crumenifer
  • Scanning electron microscopy
  • Sesbania sesban var. bicolor