Effect of ambient temperature on the proliferation of brown adipocyte progenitors and endothelial cells during postnatal BAT development in Syrian hamsters
In Syrian hamsters, brown adipose tissue (BAT) develops postnatally through the proliferation and differentiation of brown adipocyte progenitors. In the study reported here, we investigated how ambient temperature influenced BAT formation in neonatal hamsters. In both hamsters raised at 23 or 30 °C, the interscapular fat changed from white to brown coloration in an age-dependent manner and acquired the typical morphological features of BAT by day 16. However, the expression of uncoupling protein 1, a brown adipocyte marker, and of vascular endothelial growth factor α were lower in the group raised at 30 °C than in that raised at 23 °C. Immunofluorescent staining revealed that the proportion of Ki67-expressing progenitors and endothelial cells was lower in the 30 °C group than in the 23 °C group. These results indicate that warm ambient temperature suppresses the proliferation of brown adipocyte progenitors and endothelial cells and negatively affects the postnatal development of BAT in Syrian hamsters.
KeywordsAdipocyte Brown adipose tissue Uncoupling protein 1 Syrian hamster Proliferation Ambient temperature
KN, YOO, JNK, SN, and AT conducted the experiments; YOO and KK designed the experiments; KN and YOO wrote the manuscript.
This study was supported by JSPS KAKENHI Grant Numbers 17K0811807 and 15H04545, Grants-in-Aid for the Naito Foundation, and for the Akiyama Life Science Foundation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving animals were performed in accordance with the guidelines of Hokkaido University Manual for Implementing Animal Experimentation, in the animal facility approved by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International. The experimental procedures and care of animals were approved by the Animal Care and Use Committee of Hokkaido University (Hokkaido, Japan). This article does not contain any studies with human participants performed by any of the authors.
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