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The Journal of Physiological Sciences

, Volume 67, Issue 6, pp 689–698 | Cite as

Somatosensory regulation of serotonin release in the central nucleus of the amygdala is mediated via corticotropin releasing factor and gamma-aminobutyric acid in the dorsal raphe nucleus

  • Ryota Tokunaga
  • Rie Shimoju
  • Hideshi Shibata
  • Mieko Kurosawa
Original Paper
  • 272 Downloads

Abstract

Noxious cutaneous stimulation increases, whereas innocuous cutaneous stimulation decreases serotonin (5-HT) release in the central nucleus of the amygdala (CeA) in anesthetized rats. In the present study, we investigated the contribution of corticotropin releasing factor (CRF) receptors and gamma-aminobutyric acid (GABA) receptors in the dorsal raphe nucleus (DRN) to those responses. Release of 5-HT in the CeA was monitored by microdialysis before and after 10-min stimulation by pinching or stroking. Increased 5-HT release in the CeA in response to pinching was abolished by CRF2 receptor antagonism in the DRN. Decreased 5-HT release in the CeA in response to stroking was abolished by either CRF1 receptor antagonism or GABAA receptor antagonism in the DRN. These results suggest that opposite responses of 5-HT release in the CeA to noxious versus innocuous stimulation of the skin are due to separate contributions of CRF2, CRF1 and GABAA receptors in the DRN.

Keywords

CRF1 receptor CRF2 receptor GABAA receptor Microdialysis Pinching Stroking 

Notes

Acknowledgments

We thank Mr. Yuki Masuya, Eicom Co., Ltd., for his skillful technical assistance in measuring 5-HT. We also thank Mr. Noriaki Takagi for his partial contribution to this study. The present study was supported by the JSPS KAKENHI Grant (no. 16K01465) to M.K.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© The Physiological Society of Japan and Springer Japan 2016

Authors and Affiliations

  1. 1.Center for Medical ScienceInternational University of Health and WelfareOtawaraJapan
  2. 2.Division of Physical Therapy, Graduate School of Health and Welfare SciencesInternational University of Health and WelfareOtawaraJapan
  3. 3.Laboratory of Veterinary Anatomy, Institute of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  4. 4.Department of Pharmaceutical SciencesInternational University of Health and WelfareOtawaraJapan

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