The Journal of Physiological Sciences

, Volume 66, Issue 5, pp 397–405 | Cite as

Neuroprotective effects of oleuropein against cognitive dysfunction induced by colchicine in hippocampal CA1 area in rats

  • Soheila PourkhodadadEmail author
  • Masoud Alirezaei
  • Mehrnoush Moghaddasi
  • Hassan Ahmadvand
  • Manizheh Karami
  • Bahram Delfan
  • Zahra Khanipour
Original Paper


Alzheimer’s disease is a progressive neurodegenerative disorder with decline in memory. The role of oxidative stress is well known in the pathogenesis of the disease. The purpose of this study was to evaluate pretreatment effects of oleuropein on oxidative status and cognitive dysfunction induced by colchicine in the hippocampal CA1 area. Male Wistar rats were pretreated orally once daily for 10 days with oleuropein at doses of 10, 15 and 20 mg/kg. Thereafter, colchicine (15 μg/rat) was administered into the CA1 area of the hippocampus to induce cognitive dysfunction. The Morris water maze was used to assess learning and memory. Biochemical parameters such as glutathione peroxidase and catalase activities, nitric oxide and malondialdehyde concentrations were measured to evaluate the antioxidant status in the rat hippocampus. Our results indicated that colchicine significantly impaired spatial memory and induced oxidative stress; in contrast, oleuropein pretreatment significantly improved learning and memory retention, and attenuated the oxidative damage. The results clearly indicate that oleuropein has neuroprotective effects against colchicine-induced cognitive dysfunction and oxidative damage in rats.


Oleuropein Colchicine Alzheimer’s disease Oxidative stress Hippocampus 



This research was financially supported by Razi Herbal Medicines Research Center. We are most grateful to Dr. B. Rasoulian for his kind cooperation in Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences and to Mr. M. Almasian for proofreading the manuscript.


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Copyright information

© The Physiological Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Soheila Pourkhodadad
    • 1
    Email author
  • Masoud Alirezaei
    • 2
  • Mehrnoush Moghaddasi
    • 3
  • Hassan Ahmadvand
    • 4
  • Manizheh Karami
    • 5
  • Bahram Delfan
    • 3
  • Zahra Khanipour
    • 1
  1. 1.Razi Herbal Medicines Research CenterLorestan University of Medical SciencesKhorramabadIran
  2. 2.Division of Biochemistry, School of Veterinary MedicineLorestan UniversityKhorramabadIran
  3. 3.Department of Physiology and PharmacologyLorestan University of Medical SciencesKhorramabadIran
  4. 4.Department of Biochemistry, Faculty of MedicineLorestan University of Medical SciencesKhorramabadIran
  5. 5.Department of Biology, Faculty of Basic SciencesShahed UniversityTehranIran

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