Anatomical Science International

, Volume 92, Issue 3, pp 330–337 | Cite as

Quercetin ameliorates peripheral nerve ischemia–reperfusion injury through the NF-kappa B pathway

  • Mohammadreza Gholami
  • Zahra Khanipour Khayat
  • Khatereh Anbari
  • Zia Obidavi
  • Alimohammad Varzi
  • Mandana Beigi Boroujeni
  • Mohsen Alipour
  • Ali Niapoor
  • Anneh Mohammad GharraviEmail author
Original Article


This study aimed to investigate the protective effect of quercetin against ischemia–reperfusion (IR) injury induced in the sciatic nerve of the rat. Quercetin (20 mg/kg) was given during ischemia just before reperfusion. Four groups of rats (Q+IR3, Q+IR7, Q+IR14, and Q+IR28) received 3, 7, 14, and 28 days of reperfusion, respectively, after the intraperitoneal injection of quercetin. After reperfusion, a behavioral test was performed and the sciatic functional index was calculated. Each sciatic nerve was stained to check for edema and ischemic fiber degeneration. Immunohistochemical staining was performed to detect TNF-alpha and NF-kappa B, and TUNEL staining was carried out to detect apoptosis. The Q+IR3, Q+IR7, and Q+IR14 groups showed significantly increased behavioral scores and ameliorated sciatic functional index values compared to IR-injured rats that received vehicle alone during ischemia and then the same period of reperfusion. The Q+IR3, Q+IR7, Q+IR14, and Q+IR28 groups presented significant ischemic fiber degeneration (IFD), TNF-alpha expression, and apoptosis as compared with the IR-injured and perfused rats that did not receive quercetin. The Q+IR3, Q+IR7, and Q+IR28 groups also exhibited significantly decreased NF-kappa B expression (p < 0.001, p = 0.001, p = 0.026) as compared with the IR-injured rats that were perfused but did not receive quercetin. These results imply that quercetin may be beneficial in the treatment of sciatic IR injury because of its antiapoptotic and antiinflammatory effects and its ability to decrease the expression of NF-kappa B.


Quercetin Ischemia–reperfusion Sciatic functional index Ischemic fiber degeneration TNF-alpha NF-kappa B Apoptosis 



This study was approved by the Lorestan University of Medical Sciences. The authors would like to give special thanks to the Lorestan University of Medical Sciences, Khorramabad, Iran, for their financial support. The authors also thank the head and staff of the Razi Herbal Medicines Research Center of Lorestan Medical University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Japanese Association of Anatomists 2016

Authors and Affiliations

  • Mohammadreza Gholami
    • 1
  • Zahra Khanipour Khayat
    • 1
  • Khatereh Anbari
    • 2
  • Zia Obidavi
    • 1
  • Alimohammad Varzi
    • 3
  • Mandana Beigi Boroujeni
    • 1
  • Mohsen Alipour
    • 4
  • Ali Niapoor
    • 5
  • Anneh Mohammad Gharravi
    • 6
    Email author
  1. 1.Razi Herbal Medicines Research Center and Department of AnatomyLorestan University of Medical SciencesKhorramabadIran
  2. 2.Department of Social MedicineLorestan University of Medical SciencesKhorramabadIran
  3. 3.Department of ImmunologyLorestan University of Medical SciencesKhorramabadIran
  4. 4.Department of Physiology and Pharmacology, Faculty of MedicineZanjan University of Medical SciencesZanjanIran
  5. 5.Research laboratory for embryology and stem cell, Department of Anatomical Sciences and pathology, Faculty of MedicineArdabil University of Medical SciencesArdabilIran
  6. 6.Stem cells and tissue engineering research centerShahroud University of Medical SciencesShahroudIran

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