Abstract
Brown algae contain a β-glucan called laminaran that exhibits various biological activities, such as immune-enhancing, anti-cancer, and antioxidant properties. The laminaran content in brown algae varies depending on species, habitat, and season. When developing a laminaran-containing functional food using brown algae or its extract, accurate quantitative methods to determine laminaran content in a variety of brown algae and foodstuffs are required. In this study, we examined the applicability of the existing sodium hypochlorite extracting and enzymatic digesting (SEED) assay for the determination of the laminaran content of the brown alga Eisenia bicyclis. The laminaran content of E. bicyclis could be determined using the SEED assay. Furthermore, we developed a rapid and concise acid extraction enzymatic method. This method can specifically extract total water-soluble β-glucan (laminaran) and selectively convert it to glucose. The generated glucose content was determined using the glucose oxidase/peroxidase method. The validation test using brown algae E. bicyclis and Sargassum horneri revealed that the acid extraction-enzymatic method is accurate, precise, and applicable to the determination of the laminaran content in these brown algae. Furthermore, developed method was also applicable to laminaran-added processed foods.
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References
Bobadilla F, Rodriguez-Tirado C, Imarai M, Galotto MJ, Andersson R (2013) Soluble β-1,3/1,6-glucan in seaweed from the southern hemisphere and its immunomodulatory effect. Carbohydr Polym 92:241–248. https://doi.org/10.1016/j.carbpol.2012.09.071
Cotas J, Leandro A, Monteiro P, Pacheco D, Figueirinha A, Gonçalves AMM, Da Silva GJ, Pereira L (2020) Seaweed phenolics: from extraction to applications. Mar Drugs 18:1–47
Danielson ME, Dauth R, Elmasry NA, Langeslay RR, Magee AS, Will PM (2010) Enzymatic method to measure β-1,3-β-1,6-glucan content in extracts and formulated products (GEM assay). J Agric Food Chem 58:10305–10308. https://doi.org/10.1021/jf102003m
Devillé C, Damas J, Forget P, Dandrifosse G, Peulen O (2004) Laminarin in the dietary fibre concept. J Sci Food Agric 84:1030–1038. https://doi.org/10.1002/jsfa.1754
Gacesa P (1988) Alginates. Carbohydr Polym 8:161–182. https://doi.org/10.1016/0144-8617(88)90001-X
Ide M, Okumura M, Koizumi K, Kumagai M, Yoshida I, Yoshida M, Mishima T, Nakamura M (2018) Novel method to quantify β-glucan in processed foods: Sodium hypochlorite extracting and enzymatic digesting (SEED) Assay. J Agric Food Chem 66:1033–1038. https://doi.org/10.1021/acs.jafc.7b05044
Izydorczyk MS, Dexter JE (2008) Barley β-glucans and arabinoxylans: Molecular structure, physicochemical properties, and uses in food products-a Review. Food Res Int 41:850–868. https://doi.org/10.1016/j.foodres.2008.04.001
Kadam SU, O’Donnell CP, Rai DK, Rai DK, Hossain MB, Burgess C, Walsh D, Tiwari BK (2015a) Laminarin from Irish brown seaweeds Ascophyllum nodosum and Laminaria hyperborea: Ultrasound assisted extraction, characterization and bioactivity. Mar Drugs 13:4270–4280. https://doi.org/10.3390/md13074270
Kadam SU, Tiwari BK, O’Donnell CP (2015b) Extraction, structure and biofunctional activities of laminarin from brown algae. Int J Food Sci Technol 50:24–31. https://doi.org/10.1111/ijfs.12692
Kellogg J, Grace MH, Lila MA (2014) Phlorotannins from alaskan seaweed inhibit carbolytic enzyme activity. Mar Drugs 12:5277–5294. https://doi.org/10.3390/md12105277
Kwon TH, Kim TW, Kim CG, Park NH (2013) Antioxidant activity of various solvent fractions from edible brown alga, Eisenia bicyclis and its active compounds. J Food Sci 78:C679–C684. https://doi.org/10.1111/1750-3841.12109
Mabeau S, Fleurence J (1993) Seaweed in food products: biochemical and nutritional aspects. Trends Food Sci Technol 4:103–107. https://doi.org/10.1016/0924-2244(93)90091-N
Menshova RV, Ermakova SP, Anastyuk SD, Isakov VV, Dubrovskaya YV, Kusaykin MI, Um BH, Zvyagintseva TN (2014) Structure, enzymatic transformation and anticancer activity of branched high molecular weight laminaran from brown alga Eisenia bicyclis. Carbohydr Polym 99:101–109. https://doi.org/10.1016/j.carbpol.2013.08.037
Nakamura T, Nagayama K, Uchida K, Tanaka R (1996) Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicyclis. Fish Sci 62(6):923–926
Nakashima A, Yamada K, Iwata O, Sugimoto R, Atsuji K, Ogawa T, Ishibashi-Ohgo N, Suzuki K (2018) β-Glucan in foods and its physiological functions. J Nutr Sci Vitaminol (tokyo) 64:8–17. https://doi.org/10.3177/jnsv.64.8
Samuelsen ABC, Schrezenmeir J, Knutsen SH (2014) Effects of orally administered yeast-derived beta-glucans: a review. Mol Nutr Food Res 58:183–193. https://doi.org/10.1002/mnfr.201300338
Tang C, Kamiya T, Liu Y, Kadoki M, Kakuta S, Oshima K, Hattori M, Takeshita K, Kanai T, Saijo S, Ohno N, Iwakura Y (2015) Inhibition of dectin-1 signaling ameliorates colitis by inducing lactobacillus-mediated regulatory T cell expansion in the intestine. Cell Host Microbe 18:183–197. https://doi.org/10.1016/j.chom.2015.07.003
Wijesekara I, Yoon NY, Kim SK (2010) Phlorotannins from Ecklonia cava (Phaeophyceae): biological activities and potential health benefits. BioFactors 36:408–414. https://doi.org/10.1002/biof.114
Zargarzadeh M, Amaral AJR, Custódio CA, Mano JF (2020) Biomedical applications of laminarin. Carbohydr Polym 232:115774. https://doi.org/10.1016/j.carbpol.2019.115774
Zhu F, Du B, Bian Z, Xu B (2015) β-Glucans from edible and medicinal mushrooms: characteristics, physicochemical and biological activities. J Food Compos Anal 41:165–173. https://doi.org/10.1016/j.jfca.2015.01.019
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This study was supported by the Sankei Science Scholarship Foundation.
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Kumagai, M., Ogasawara, K., Ide, M. et al. Acid extraction enzymatic method to measure laminaran in edible brown algae. Fish Sci 89, 409–414 (2023). https://doi.org/10.1007/s12562-023-01684-8
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DOI: https://doi.org/10.1007/s12562-023-01684-8