Abstract
The eyestalk of crustaceans is a vital neuroendocrine organ complex that regulates various important physiological processes, especially molting and reproduction. The mandibular organ-inhibiting hormone (MOIH), an important member of the crustacean hyperglycemic hormone (CHH) neuropeptide family, mediates vitellogenesis and reproduction. In this study, EsMOIH was isolated from the eyestalk of Eriocheir sinensis. Sequence analysis suggested that EsMOIH belongs to CHH subfamily II and contains a conserved CHH/molt-/vitellogenesis-inhibiting hormone (MIH/VIH) domain and six conserved cysteines forming three disulfide bonds. The findings corroborated the results of phylogenetic analysis. Quantitative polymerase chain reaction (qPCR) analysis suggested that EsMOIH was ubiquitously expressed in all the examined tissues, but was highly specifically expressed in the hepatopancreas. The hepatopancreatic transcription of EsMOIH decreased significantly at stages III and IV, but increased thereafter and was highest at stage V. EsMOIH transcription was strongly negatively correlated with the hepatopancreatic expression of vitellogenin (EsVg), suggesting that EsMOIH could partake in inhibiting vitellogenesis in the hepatopancreas of E. sinensis. The RNA interference (RNAi)-mediated specific knockdown of EsMOIH significantly reduced EsMOIH expression in vitro and in vivo, but markedly increased EsVg expression in the ovary and hepatopancreas. Altogether, the results suggested that EsMOIH partakes in inhibiting vitellogenesis in E. sinensis.
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Acknowledgements
This research was supported by Open Funding Project of the Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs [K2022-04], Anhui Provincial College Students Innovation and Entrepreneurship Project [S202110364022], Key R and D Program of Chuzhou [2020ZN005], and Fishery Industrial System of Anhui Province, China [[2021]711]. Thanks to Xuancheng Jinxin crab seed professional cooperative for providing experimental animals.
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Open Funding Project of the Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs K2022-04, Xilei Li, Anhui Provincial College Students Innovation and Entrepreneurship Project [S202110364022], Xilei Li, Key R and D Program of Chuzhou [2020ZN005], Xilei Li, and Fishery Industrial System of Anhui Province, China, China [[2021]711], Xilei Li.
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Ding, S., Huang, M., Sheng, N. et al. RNAi-mediated knockdown of the mandibular organ-inhibiting hormone (MOIH) gene stimulates vitellogenesis in the Chinese mitten crab Eriocheir sinensis. Fish Sci 89, 399–408 (2023). https://doi.org/10.1007/s12562-023-01680-y
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DOI: https://doi.org/10.1007/s12562-023-01680-y