Abstract
With the increased incidence of lifestyle-related diseases, prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing at an unprecedented rate, leading to rising interest in studying the role of functional foods in its prevention. This study demonstrated the efficacy of salmon milt deoxyribonucleic acid (SM DNA) in preventing liver dysfunction in a diet-induced obesity model. Seven-week-old male C57BL/6J mice were fed normal diet (ND), high-fat diet (HFD), and HFD supplemented with 3% SM DNA (HFD + SM DNA) for 12 weeks. Increased body weight, fat-to-body weight ratio, and fat accumulation in HFD-fed mice demonstrated successful establishment of an obesity model. The HFD group showed increased alanine aminotransferase (ALT) levels, a marker of liver function, and NAFLD activity score (NAS) based on histological observations showed steatosis and mild inflammation but no fibrosis, indicating NAFLD but no nonalcoholic steatohepatitis. In the HFD + SM DNA group, the improved superoxide dismutase activity and NAFLD activity score suggested that SM DNA prevented liver dysfunction while maintaining antioxidant capacity and suppressing intrahepatocyte fat droplet formation. SM DNA attenuated fat accumulation in the liver and improved hepatic function. This study explored the potential of SM DNA as a functional food to prevent NAFLD and other visceral obesity-related diseases.
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All the authors contributed to the conception and design of the study. Asami Michitatsu, Tatsuya Konishi, and Yoshinori Takahashi performed material preparation, data collection, and analysis. The first draft of the manuscript was written by Asami Michitatsu and Yoshinori Takahashi. All authors commented on the previous versions of the manuscript. All authors have read and approved the final manuscript.
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All experiments were performed in accordance with protocols and guidelines approved by the Animal Care Committee of Maruha Nichiro Corporation.
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Michitatsu, A., Konishi, T. & Takahashi, Y. Dietary salmon milt deoxyribonucleic acid prevents hepatosteatosis in mice. Fish Sci 89, 83–91 (2023). https://doi.org/10.1007/s12562-022-01645-7
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DOI: https://doi.org/10.1007/s12562-022-01645-7