Abstract
Although fish roes (or the egg-laden ovary) are seafood products with high nutritional value and are considered abundant in vitamins including vitamin B12, nevertheless, the detailed properties of vitamin B12 have not been fully elucidated in fish roe products. Vitamin B12 content was determined using C18-reversed-phase high-performance liquid chromatography after purification of samples from immunoaffinity columns. Salmon egg-laden ovary products (sujiko), salmon roe products (ikura), dried mullet roe products (bottarga), and white sturgeon roe products (caviar) were found to contain substantial levels of vitamin B12 (more than approximately 15 µg/100 g wet weight). Interestingly, high levels of vitamin B12 per 100 g wet weight have been measured in pollack roe products (tarako) and flying fish roe products (tobiko). Liquid chromatography–electrospray-ionization/tandem mass spectrometry analysis revealed that vitamin B12 was the predominant corrinoid in the evaluated fish roe products examined, while no inactive corrinoid compounds were identified. These results suggest that commercially available fish roe products could be an important source of vitamin B12 for use as a dietary product for humans.
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References
Bennink MR, Ono K (1982) Vitamin B12, E and D content of raw and cooked beef. J Food Sci 47:1786–1792
Bito T, Tanioka Y, Watanabe F (2018) Characterization of vitamin B12 compounds from marine foods. Fish Sci 84:747–755
Bledsoe G, Bledsoe C, Rasco BA (2003) Caviars and fish roe products. Crit Rev Food Sci Nutr 43:317–356
Bourre JME, Paquotte PM (2008) Contribution (in 2005) of marine and fresh water products (finfish and shellfish, seafood, wild and formed) to the French dietary intakes of vitamin D and B12, selenium, iodine and docosahexaenoic acid: impact on public health. Int J Food Nutr 59:491–501
Brouwer-Brolsma EM, Dhonukshe-Rutten RAM, van Wijngaarden JP, van der Zwaluw NL, van der Velde N, de Groot LCPGM (2015) Dietary sources of vitamin B12 and their association with vitamin B12 status markers in healthy older adults in the B-PROOF study. Nutrients 7:7781–7797
Farag MA, Abib B, Tawfik S, Shafik N, Khattab AR (2021) Caviar and fish roe substitutes: current status of their nutritive value, bio-chemical diversity, authenticity and quality control methods with future perspectives. Trend Food Sci Technol 110:405–417
Guggisberg D, Risse MC, Hadorn R (2012) Determination of vitamin B12 in meat products by RP-HPLC after enrichment and purification on an immunoaffinity column. Meat Sci 90:279–283
Heal KR, Qin W, Ribalet F, Bertagnolli AD, Coyote-Maestas W, Hmelo LR, Moffett JW, Devol AH, Armbrust EV, Stahl DA, Ingalls AE (2017) Two distinct pools of B12 analogs reveal community interdependencies in the ocean. Proc Natl Acad Sci USA 114:364–369
Heudi O, Kilinç T, Fontannaz P, Marley E (2006) Determination of vitamin B12 in food products and in premixes by reversed-phase high performance liquid chromatography and immunoaffinity extraction. J Chromatogr A 1101:63–68
Institute of Medicine. Vitamin B12 (1998) Dietary reference intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline. Institute of Medicine, National Academy Press, Washington, pp 306–356
Koseki K, Okamoto N, Ito Y, Bito T, Ishihara A, Umebayashi Y, Watanabe F (2021) Vitamin B12 carboxylic acid characterization in the viscera edible portions of lobster, crayfish, crab, and shrimp. ACS Food Sci Technol 1:1523–1528
Koseki K, Namura M, Bito T, Umebayashi Y, Watanabe F (2022) Characterization of vitamin B12 compounds in commercially available livestock livers used as foods. ACS Food Sci Technol 2:1364–1370. https://doi.org/10.1021/acsfoodscitech.2c00172
Nishioka M, Kanosue F, Yabuta Y, Watanabe F (2011) Loss of vitamin B12 in fish (round herring) meats during various cooking treatments. J Nutr Sci Vitaminol 57:432–436
Okamoto N, Nagao F, Umebayashi Y, Bito T, Prangthip P, Watanabe F (2021) Pseudovitamin B12 and factor S are the predominant corrinoid compounds in edible cricket products. Food Chem 347:129048
Rosa A, Isola R, Nieddu M, Masala C (2020) The role of lipid composition in the sensory attributes and acceptability of the salted and dried mullet rose (bottarga): a study in human and animal models. Nutrients 12:3454
Scheers N, Lindqvist H, Langkilde AM, Undeland I, Sandberg AS (2014) Vitamin B12 as a potential compliance marker for fish intake. Eur J Nutr 53:1327–1333
Shibata K, Fukuwatari T, Imai E, Hayakawa T, Watanabe F, Takimoto H, Watanabe T, Umegaki K (2013) Dietary reference intakes for Japanese 2010: water-soluble vitamins. J Nutr Sci Vitaminol 59:S67–S82
Tanioka Y, Takenaka S, Furusho T, Yabuta Y, Nakano Y, Watanabe F (2014) Identification of vitamin B12 and pseudovitamin B12 from various edible shellfish using liquid chromatography-electrospray ionization/tandem mass spectrometry. Fish Sci 80:1065–1071
The Council for Science and Technology, Standard Tables of Food Composition in Japan-2020 (2020), Ministry of Education, Culture, Sports, Science and Technology, Japan. Tokyo, Official gazette co-operation of Japan, pp 156–185
Vogiatzoglou A, Smith AD, Nurk E, Berstad P, Crevon CA, Ueland PM, Vollset SE, Tell GS, Refsum H (2009) Dietary sources of vitamin B12 and their association with plasma vitamin B12 concentrations in the general population: the Hordaland Homocyteine Study. Am J Clin Nutr 89:1078–1097
Watanabe F, Bito T (2018) Determination of cobalamin and related compounds in foods. J AOAC Int 101:1308–1313
Watanabe F, Abe K, Fujita T, Goto M, Hiemori M, Nakano Y (1998) Effects of microwave heating on the loss of vitamin B12 in foods. J Agric Food Chem 46:206–210
Watanabe F, Katsura H, Takenaka S, Fujita T, Abe K, Tamura Y, Nakatsuka T, Nakano Y (1999) Pseudovitamin B12 is the predominate cobamide of an algal health food, spirulina tablets. J Agric Food Chem 47:4736–4741
Watanabe F, Katsura H, Takenaka S, Enomoto T, Nakatsuka T, Nakano Y (2001) Characterization of vitamin B12 compounds from edible shellfish, clam, oyster, and mussel. Int J Food Sci Nutr 52:263–268
Watanabe F, Yabuta Y, Bito T, Teng F (2014) Vitamin B12-containing plant food sources for vegetarians. Nutrients 6:1861–1873
Yoshino K, Inagawa M, Oshima M, Yokota K, Umesawa M, Endo M, Yamagashi K, Tanigawa T, Sato S, Shimamoto T, Iso H (2005) Trends in dietary intake of folate, vitamin B6 and vitamin B12 among Japanese adults in two rural communities from 1971 through 2001. J Epidemiol 15:29–37
Acknowledgements
This work was supported by the Japan Society for the Promotion of Science KAKENHI grant number 21K05488 (F.W.). We would like to thank Enago (www.enago.jp) for English language editing.
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Yamanaka, T., Namura, M., Koseki, K. et al. Characterization of vitamin B12 compounds from commercially available fish roe products. Fish Sci 88, 815–820 (2022). https://doi.org/10.1007/s12562-022-01636-8
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DOI: https://doi.org/10.1007/s12562-022-01636-8