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Low- and high-temperature processed potato protein concentrates as alternatives to fishmeal in greater amberjack Seriola dumerili diets


Potato protein concentrate (PPC) is a potential fish meal (FM) substitute. We replaced FM in greater amberjack diets with low-temperature air-dried PPC (LT-PPC) and high-temperature rotary-dried PPC (HT-PPC) to investigate their effects on growth and feed utilization. Four experimental and one control diet was prepared. The control diet comprised FM, wherein the experimental diets were prepared by replacing 20 and 40% of FM in the control diet with LT-PPC (LP20 and LP40) or HT-PPC (LP20 and LP40). Following an 8-week growth trial, the final mean body weight, specific growth rate, and feed intake of HP40 diet-fed fish were significantly lower than those of control diet-fed fish (p < 0.05). However, these parameters in LT-PPC and HP20 diet-fed fish did not differ significantly from those of the control diet-fed fish (p > 0.05). In vitro relative protein digestibility did not differ between LT-PPC (91.1%) and HT-PPC (89.5%) (p > 0.05); however, the digestion speed of LT-PPC was higher (63.8%) than that of HT-PPC (46.8%) (p < 0.05). In conclusion, the findings demonstrate that LT-PPC and HT-PPC could be used to replace up to 40 and 20% of FM in greater amberjack diets, respectively, without compromising growth performance.

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Data availability

The datasets generated and analyzed during the current study are available from the corresponding author upon reasonable request.


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This study was funded by Kaneka Corp. (Japan) through a joint research alliance between Kaneka and Kindai University. The authors are grateful to all staff and students at the Aquaculture Research Institute at the Uragami Station (Kindai University, Japan) for their support during this study.

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Authors and Affiliations



FT: conceptualization, Validation, Formal analysis, Investigation, Writing—Original Draft, Supervision, Project administration. KS: resources. HM: formal analysis, Investigation. SY: investigation. AB: investigation, Writing—Review and Editing. HT: investigation, Writing—Review and Editing.

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Correspondence to Fumiaki Takakuwa.

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The authors confirm that the study was approved by the institutional ethical review committee, and all experiments were conducted according to the ‘Guidelines for Animal Experimentation at the Aquaculture Research Institute of Kindai University.

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Takakuwa, F., Suzuri, K., Mikotaka, H. et al. Low- and high-temperature processed potato protein concentrates as alternatives to fishmeal in greater amberjack Seriola dumerili diets. Fish Sci 88, 581–592 (2022).

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  • Greater amberjack
  • Growth metrics
  • Digestibility
  • Nutrient bioavailability
  • Proximate chemical composition
  • Intestinal histology