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Application of CHAPS-PC detergent to determine rhodopsin absorbance spectra in squids

Abstract

The utility of CHAPS-PC, a detergent combination for solubilizing membrane proteins, was investigated as a substitute to digitonin solution for extracting rhodopsin in the measurement of spectral sensitivity in squid. Microvilli of the photoreceptor outer segments were collected and purified from the retinas of three species, Uroteuthis edulis, Todarodes pacificus, and Heterololigo bleekeri, using sucrose density-gradient centrifugation. The difference in the absorption maximum (λmax) of rhodopsin between extraction with CHAPS-PC or with digitonin was 0–3 nm for all species. The λmax values of the three squid species were 488–490, 478–480, and 488–491 nm, respectively, which aligns well with values reported in previous studies. CHAPS and PC are less expensive than digitonin, which is advantageous when conducting repeated experiments. We hope that the present procedure for the extraction of visual pigments offers an improved fisheries technology that will be used to better understand the ecology of diverse squid species.

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Acknowledgements

We would like to thank Dr E Hasegawa for his advice on creating the protocol. Cynthia Kulongowski with Edanz (https://jp.edanz.com/ac) edited the language of a draft of this manuscript.

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Correspondence to Taeko Miyazaki.

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Miyazaki, T., Osumi, H. Application of CHAPS-PC detergent to determine rhodopsin absorbance spectra in squids. Fish Sci 88, 485–492 (2022). https://doi.org/10.1007/s12562-022-01603-3

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  • DOI: https://doi.org/10.1007/s12562-022-01603-3

Keywords

  • Squid
  • Rhodopsin
  • Spectral sensitivity
  • Visual pigment extraction
  • CHAPS
  • Digitonin