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Oocyte and embryonic development, size at sexual maturity, and spawning season of Charybdis bimaculata

Abstract

This study describes the first thorough investigation of oocyte and embryonic development as well as histologically confirmed size at sexual maturity of female Charybdis bimaculata. A total of 1211 females were collected from the central part of Kagoshima Bay, southern Japan, from October 2018 to January 2020. Six sequential oocyte developmental stages were confirmed by histological examination. C. bimaculata exhibited asynchronous-type ovarian development. Therefore, ovarian maturity status (immature, maturing, and mature) was determined from the most advanced oocyte found in the ovary. Females having oocytes evidencing either germinal vesicle breakdown or migratory nucleus were defined as mature. Mature females ranged from 19.0 to 30.6 mm in carapace width. The size at sexual maturity was estimated to be 21.8 mm in carapace width based on the 50% maturity size. The spawning season was determined from May to November when mature females occurred. Microscopic observation confirmed four sequential embryonic developmental stages. The majority of females with final-stage embryos had mature ovaries signifying continuity of reproduction. Asynchronous-type ovary, continuous reproduction, and consecutive broods suggest multiple spawning in a single spawning season. The incubation period was estimated to be under 1 month for C. bimaculata.

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Acknowledgements

We are grateful to Dr. Md. Mosaddequr Rahman, Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University for proofreading to improve the English of the manuscript. Thanks are due to the crew of the training vessel Nansei Maru, Faculty of Fisheries, Kagoshima University for their kind cooperation during the experimental trawl surveys. We would like to thank the members of the Fisheries Biology Laboratory of the same institution as well for their cordial assistance throughout the study. We would like to express our gratitude to the reviewers and the editor for their generous comments and suggestions to improve the manuscript. This work was supported in part by JSPS KAKENHI grant number 19K02297.

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Correspondence to Jun Ohtomi.

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Nawer, F., Doi, W. & Ohtomi, J. Oocyte and embryonic development, size at sexual maturity, and spawning season of Charybdis bimaculata. Fish Sci 88, 449–459 (2022). https://doi.org/10.1007/s12562-022-01601-5

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  • DOI: https://doi.org/10.1007/s12562-022-01601-5

Keywords

  • Charybdis bimaculata
  • Embryo
  • Maturation
  • Oocyte
  • Reproduction
  • Size at sexual maturity
  • Spawning season