Abstract
In order to study the effects of carbon source addition on the bacterial community, water quality and shrimp growth in the recirculating aquaculture system of Litopenaeus vannamei, sucrose with 50% of the daily feed was added daily to the system from the middle stage of culture. The bacterial communities in biofilters, aquaculture waters and shrimp intestines were measured using an Illumina MiSeq platform. The addition of sucrose did not significantly improve the regulation of nitrogen in the water, although there was a tendency to increase shrimp production. The addition of sucrose also had little effect on the bacterial community of water and intestine, but had significant effects on the bacterial community in the biofilter. Carbon addition transformed the dominant bacterial taxa of the biofilter from the Proteobacteria dominated by Rhodobacteraceae to the Planctomycetes dominated by Planctomycetaceae. In this study, the characteristics of the bacterial community and the effects of carbon source addition in the recirculating aquaculture system of L. vannamei are presented, which provides a basis for microbial management in the recirculating aquaculture system of L. vannamei. In view of the unknown role of numerous bacterial groups, the function of the bacterial community needs further study.
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References
Abu Bakar NS, Nasir NM, Lananan F, Hamid SHA, Lam SS, Jusoh A (2015) Optimization of C/N ratios for nutrient removal in aquaculture system culturing African catfish, (Clarias gariepinus) utilizing bioflocs technology. Int Biodeterior Biodegrad 102:100–106
APHA (1981) Standard methods for the examination of water and wastewater. APHA American Public Health Association, Baltimore
Attramadal KJK, Oie G, Storseth TR, Alver MO, Vadstein O, Olsen Y (2012) The effects of moderate ozonation or high intensity UV-irradiation on the microbial environment in RAS for marine larvae. Aquaculture 330:121–129
Avnimelech Y (1999) Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176:227–235
Badiola M, Mendiola D, Bostock J (2012) Recirculating aquaculture systems (RAS) analysis: main issues on management and future challenges. Aquac Eng 51:26–35
Bendschneider K, Robinson RJ (1952) A new spectrophotometric method for the determination of nitrite in sea water. J Mar Res 11:87–96
Brown MN, Briones A, Diana J, Raskin L (2013) Ammonia-oxidizing archaea and nitrite-oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system. FEMS Microbiol Ecol 83:17–25
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Tumbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Meth 7:335–336
Chao A (1984) Nonparametric estimation of the number of classes in a population. Scand J Stat 11:265–270
Chen Z, Ge H, Chang Z, Song X, Zhao F, Li J (2018) Nitrogen budget in recirculating aquaculture and water exchange systems for culturing Litopenaeus vannamei. J Ocean Univ China 17:905–912
Fan L, Li QX (2019) Characteristics of intestinal microbiota in the Pacific white shrimp Litopenaeus vannamei differing growth performances in the marine cultured environment. Aquaculture 505:450–461
Fan L, Wang Z, Chen M, Qu Y, Li J, Zhou A, Xie S, Zeng F, Zou J (2019) Microbiota comparison of Pacific white shrimp intestine and sediment at freshwater and marine cultured environment. Sci Total Environ 657:1194–1204
Frank CS, Langhammer P, Fuchs BM, Harder J (2011) Ammonium and attachment of Rhodopirellula baltica. Arch Microbiol 193:365–372
Gainza O, Ramirez C, Salinas Ramos A, Romero J (2018) Intestinal microbiota of white shrimp Penaeus vannamei under intensive cultivation conditions in Ecuador. Microb Ecol 75:562–568
Gao L, Shan H-W, Zhang T-W, Bao W-Y, Ma S (2012) Effects of carbohydrate addition on Litopenaeus vannamei intensive culture in a zero-water exchange system. Aquaculture 342:89–96
Gao S, Pan L, Huang F, Song M, Tian C, Zhang M (2019) Metagenomic insights into the structure and function of intestinal microbiota of the farmed Pacific white shrimp (Litopenaeus vannamei). Aquaculture 499:109–118
Hou D, Huang Z, Zeng S, Liu J, Wei D, Deng X, Weng S, He Z, He J (2017) Environmental factors shape water microbial community structure and function in shrimp cultural enclosure ecosystems. Front Microbiol 8:2359–2370
Huang Z, Wan R, Song X, Liu Y, Hallerman E, Dong D, Zhai J, Zhang H, Sun L (2016) Metagenomic analysis shows diverse, distinct bacterial communities in biofilters among different marine recirculating aquaculture systems. Aquac Int 24:1393–1408
Jurasinski G, Retzer V, Beierkuhnlein C (2009) Inventory, differentiation, and proportional diversity: a consistent terminology for quantifying species diversity. Oecologia 159:15–26
Kim S-K, Pang Z, Seo H-C, Cho Y-R, Samocha T, Jang I-K (2014) Effect of bioflocs on growth and immune activity of Pacific white shrimp, Litopenaeus vannamei postlarvae. Aquac Res 45:362–371
Lin YC, Chen JC (2001) Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels. J Exp Mar Biol Ecol 259:109–119
Lin YC, Chen JC (2003) Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture 224:193–201
Lozupone C, Knight R (2005) UniFrac: a new phylogenetic method for comparing microbial communities. Appl Environ Microbiol 71:8228–8235
Michaud L, Blancheton JP, Bruni V, Piedrahita R (2006) Effect of particulate organic carbon on heterotrophic bacterial populations and nitrification efficiency in biological filters. Aquac Eng 34:224–233
Michaud L, Lo Giudice A, Interdonato F, Triplet S, Ying L, Blancheton JP (2014) C/N ratio-induced structural shift of bacterial communities inside lab-scale aquaculture biofilters. Aquac Eng 58:77–87
Nootong K, Pavasant P, Powtongsook S (2011) Effects of organic carbon addition in controlling inorganic nitrogen concentrations in a biofloc system. J World Aquacult Soc 42:339–346
Ohashi A, deSilva DGV, Mobarry B, Manem JA, Stahl DA, Rittmann BE (1995) Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs. Water Sci Technol 32:75–84
Peiffer JA, Spor A, Koren O, Jin Z, Tringe SG, Dangl JL, Buckler ES, Ley RE (2013) Diversity and heritability of the maize rhizosphere microbiome under field conditions. Proc Natl Acad Sci USA 110:6548–6553
Pfeffer CS, Hite MF, Oliver JD (2003) Ecology of Vibrio vulnificus in estuarine waters of eastern North Carolina. Appl Environ Microbiol 69:3526–3531
Rafael Martinez-Cordova L, Martinez-Porchas M, Antonio Porchas-Cornejo M, Gollas-Galvan T, Scheuren-Acevedo S, Antonio Arvayo M, Antonio Lopez-Elias J, Antonio Lopez-Torres M (2017) Bacterial diversity studied by next-generation sequencing in a mature phototrophic Navicula sp-based biofilm promoted into a shrimp culture system. Aquac Res 48:2047–2054
Ray AJ, Lotz JM (2017) Shrimp (Litopenaeus vannamei) production and stable isotope dynamics in clear-water recirculating aquaculture systems versus biofloc systems. Aquac Res 48:4390–4398
Ruan Y-J, Guo X-S, Ye Z-Y, Liu Y, Zhu S-M (2015) Bacterial community analysis of different sections of a biofilter in a full-scale marine recirculating aquaculture system. N Am J Aquac 77:318–326
Rud I, Kolarevic J, Holan AB, Berget I, Calabrese S, Terjesen BF (2017) Deep-sequencing of the bacterial microbiota in commercial-scale recirculating and semi-closed aquaculture systems for Atlantic salmon post-smolt production. Aquac Eng 78:50–62
Rurangwa E, Verdegem MCJ (2015) Microorganisms in recirculating aquaculture systems and their management. Rev Aquac 7:117–130
Schmidt V, Amaral-Zettler L, Davidson J, Summerfelt S, Good C (2016) Influence of fishmeal-free diets on microbial communities in Atlantic salmon (Salmo salar) recirculation aquaculture systems. Appl Environ Microbiol 82:4470–4481
Schneider O, Chabrillon-Popelka M, Smidt H, Haenen O, Sereti V, Eding EH, Verreth JAJ (2007) HRT and nutrients affect bacterial communities grown on recirculation aquaculture system effluents. FEMS Microbiol Ecol 60:207–219
Schveitzer R, Arantes R, Costodio PFS, do Espirito Santo CM, Arana LV, Seiffert WQ, Andreatta ER (2013) Effect of different biofloc levels on microbial activity, water quality and performance of Litopenaeus vannamei in a tank system operated with no water exchange. Aquac Eng 56:59–70
Shannon CE (1997) The mathematical theory of communication (reprinted). M D Computing 14:306–317
Shu Q, Xiong W, Peng S, Huang P (2011) Molecular progresses of marine planctomycetes: a review. Afr J Microbiol Res 5:6018–6023
Simpson EH (1949) The measurement of diversity. Nature 163:688
Sorokin DY, Vejmelkova D, Luecker S, Streshinskaya GM, Rijpstra WIC, Damste JSS, Kleerbezem R, van Loosdrecht M, Muyzer G, Daims H (2014) Nitrolancea hollandica gen. nov., sp nov., a chemolithoautotrophic nitrite-oxidizing bacterium isolated from a bioreactor belonging to the phylum Chloroflexi. Int J Syst Evol Microbiol 64:1859–1865
Thompson FL, Iida T, Swings J (2004) Biodiversity of vibrios. Microbiol Mol Biol Rev 68:403
Truong-Giang H, Hu S-Y, Chiu C-S, Truong Q-P, Liu C-H (2019) Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide. Aquac Res 50:807–817
Turner JW, Good B, Cole D, Lipp EK (2009) Plankton composition and environmental factors contribute to vibrio seasonality. ISME J 3:1082–1092
Xiong J, Zhu J, Wang K, Wang X, Ye X, Liu L, Zhao Q, Hou M, Qiuqian L, Zhang D (2014) The temporal scaling of bacterioplankton composition: high turnover and predictability during shrimp cultivation. Microb Ecol 67:256–264
Xu W-J, Morris TC, Samocha TM (2016) Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture 453:169–175
Zamora L, Vela AI, Sanchez-Porro C, Palacios MA, Dominguez L, Moore ERB, Ventosa A, Fernandez-Garayzabal JF (2013) Characterization of flavobacteria possibly associated with fish and fish farm environment. description of three novel Flavobacterium species: Flavobacterium collinsii sp nov., Flavobacterium branchiarum sp nov., and Flavobacterium branchiicola sp nov. Aquaculture 416:346–353
Zeng S, Huang Z, Hou D, Liu J, Weng S, He J (2017) Composition, diversity and function of intestinal microbiota in pacific white shrimp (Litopenaeus vannamei) at different culture stages. Peer J 5
Zeng S, Hou D, Liu J, Ji P, Weng S, He J, Huang Z (2019) Antibiotic supplement in feed can perturb the intestinal microbial composition and function in Pacific white shrimp. Appl Microbiol Biotechnol 103:3111–3122
Zhang H, Sun Z, Liu B, Xuan Y, Jiang M, Pan Y, Zhang Y, Gong Y, Lu X, Yu D, Kumar D, Hu X, Cao G, Xue R, Gong C (2016) Dynamic changes of microbial communities in Litopenaeus vannamei cultures and the effects of environmental factors. Aquaculture 455:97–108
Zhu SM, Chen SL (2001) Effects of organic carbon on nitrification rate in fixed film biofilters. Aquac Eng 25:1–11
Zhu J, Dai W, Qiu Q, Dong C, Zhang J, Xiong J (2016) Contrasting ecological processes and functional compositions between intestinal bacterial community in healthy and diseased shrimp. Microb Ecol 72:975–985
Zure M, Fernandez-Guerra A, Munn CB, Harder J (2017) Geographic distribution at subspecies resolution level: closely related Rhodopirellula species in European coastal sediments. ISME J 11:478–489
Acknowledgements
This study was supported by the National Key R&D Program of China (no. 2019YFD0900403); the China Agriculture Research System (no. CARS-48); the Central Public-interest Scientific Institution Basal Research Fund, CAFS (no. 2019CY0101); the Aoshan Innovation Project of Qingdao National Laboratory for Marine Science and Technology (no. 2015ASKJ02); and the Taishan Industrial Leader Talent Project of Shandong Province (no. LJNY 2015002).
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Chen, Z., Chang, Z., Zhang, L. et al. Effects of carbon source addition on microbial community and water quality in recirculating aquaculture systems for Litopenaeus vannamei. Fish Sci 86, 507–517 (2020). https://doi.org/10.1007/s12562-020-01423-3
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DOI: https://doi.org/10.1007/s12562-020-01423-3