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The role of the chondroitin sulfate synthase-1 gene in the immune response of the pearl oyster Pinctada fucata

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Abstract

Chondroitin sulfate synthase-1 (ChSy-1) plays an important role in the synthesis and chain extension of chondroitin sulfate (CS). CS, a linear polysaccharide with high structural diversity, plays crucial roles in cell adhesion, morphogenesis and cell division in response to viral and bacterial infections. However, the role of ChSy-1 in the immune response remains unknown. In the present study, ChSy-1 from Pinctada fucata was identified. The deduced amino acid sequence of ChSy-1 contains a conserved domain, CHGN, which belongs to glycosyltransferase family A, and multiple sequence alignment revealed that CHGN shows evolutionary differences. Phylogenetic analysis indicated that ChSy-1 may be conserved evolutionarily. The nucleus grafting experiment showed that ChSy-1 possibly participates in wound repair and immune response to the graft. Lipopolysaccharide or polyinosinic:polycytidylic acid challenge and gene expression analysis especially suggested that ChSy-1 is involved in the immune response. The results of the present study indicate that ChSy-1 plays a role in the protection of the pearl oyster against infectious diseases.

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Acknowledgements

We thank International Science Editing (https://www.internationalscienceediting.com) for editing this manuscript.

Funding

This study was supported by the earmarked fund for the Modern Agro-industry Technology Research System (grant no. CARS-49).

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  1. Hua Zhang and Xuejie Yi contributed to this work equally.

Authors and Affiliations

  1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, Guangdong, China

    Hua Zhang, Xuejie Yi, Yunyan Guan, Yu Shi, Zekui Ou & Maoxian He

  2. University of the Chinese Academy of Sciences, Beijing, 100049, China

    Xuejie Yi & Zekui Ou

  3. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, China

    Hua Zhang, Yunyan Guan, Yu Shi & Maoxian He

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  1. Hua Zhang
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Correspondence to Maoxian He.

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Zhang, H., Yi, X., Guan, Y. et al. The role of the chondroitin sulfate synthase-1 gene in the immune response of the pearl oyster Pinctada fucata. Fish Sci 86, 487–494 (2020). https://doi.org/10.1007/s12562-020-01420-6

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