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Photosynthetic bacteria enhanced water quality and integrity of microbial community composition of integrated multitrophic aquaculture system of milkfish Chanos chanos coastal farming

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  • Aquaculture
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Abstract

Aquaculture faces substantial challenges in mitigating the detrimental environmental impacts of intensive farming. Traditional methods have given rise to serious concerns, leading to the search for alternative approaches using probiotics. An indigenously isolated photosynthetic purple bacterium, Rhodovulum sulfidophilum, was introduced into a marine integrated multitrophic aquaculture system cultivating Chanos chanos in southwestern Taiwan. The effects of R. sulfidophilum on water quality parameters, the relative levels of nitrogenase-encoding nifH and nitrous oxide reductase-encoding nosZ genes, the occurrence of sulfonamide resistance, the presence of sulfonamide resistance genes, and microbial community structure were determined. Supplementation with R. sulfidophilum decreased the chemical oxygen demand, the nitrate levels, and the occurrence of sulfonamide-resistant bacteria in the fishpond water compared with that of the control after rearing. The level of the sulfonamide resistance gene sul2 decreased in R. sulfidophilum-supplemented water samples, while the control exhibited an elevated level of sul2 after rearing. Supplementation with R. sulfidophilum also maintained the integrity of the bacterial community structure. In conclusion, our results suggest that R. sulfidophilum is an attractive supplement for enhanced disease emergence control, microbial biodiversity maintenance, and sustainable marine aquaculture practice.

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Acknowledgements

The authors would like to thank Chia-Hsin Hu (Soochow University, Taiwan) and Dr. Su-Jung Chang (COA, Taiwan) for their assistance in sampling. Our gratitude also goes to Ya-Chen Lin (Soochow University, Taiwan) for her excellent artwork in the schematic illustration.

Funding

This work was funded in part by the National Ministry of Science and Technology (MOST), Republic of China (MOST 106-2632-B-031-001, MOST 105-2632-B-031-001).

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Authors and Affiliations

  1. Department of Microbiology, Soochow University, 70 Linhsi Road, Shih-Lin District, Taipei, 11102, Taiwan

    Chingwen Ying, Man-Jung Chang, Yi-Tang Chang & Wei-Liang Chao

  2. Mariculture Research Center, Fisheries Research Institute, Council of Agriculture, Tainan, 724, Taiwan

    Shinn-Lih Yeh

  3. Department of Animal Sciences, National Taiwan University, Taipei, 111, Taiwan

    Jih-Tay Hsu

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  1. Chingwen Ying
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  2. Man-Jung Chang
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  3. Yi-Tang Chang
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Correspondence to Chingwen Ying.

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Ying, C., Chang, MJ., Chang, YT. et al. Photosynthetic bacteria enhanced water quality and integrity of microbial community composition of integrated multitrophic aquaculture system of milkfish Chanos chanos coastal farming. Fish Sci 86, 329–338 (2020). https://doi.org/10.1007/s12562-019-01387-z

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