Fisheries Science

, Volume 84, Issue 5, pp 837–848 | Cite as

Lineage specific detection of the scaly form of the pen shell Atrina spp. by a loop-mediated isothermal amplification method

  • Kazumasa HashimotoEmail author
  • Katsumasa Yamada
  • Akira Nagae
  • Yukihiko Matsuyama
Original Article Biology


Taxonomy of the pen shell (genus Atrina) in East Asia has been confused because of the plasticity of the shell morphology and lack of unified morphological characteristics. We analyzed the mitochondrial cytochrome c oxidase subunit I (COI) sequence of the pen shell sampled from Ariake Bay and Seto Inland Sea, western Japan. All individuals were identified as the scaly form based on external morphology; however, their COI sequences were comprised of two distinct lineages. The sequence divergence between the two was 8.3%, indicating the high possibility that they are different species. The scaly form of the pen shell in Japan is one of the target species for resource enhancement, therefore COI lineage identification of broodstock individuals is critically important to avoid producing hybrid seed. We developed a loop-mediated isothermal amplification method that enables specific detection of the two lineages. This method can contribute to reducing the cost and time required for genotyping broodstock individuals prior to seed production.


Pen shell Genotype identification LAMP 



We are indebted to Mr. Hirokazu Miyamoto, Mr. Tatsuhito Matoba and Mr. Munenori Hayashi who offered us bivalve samples. Mr. Hiroshi Aramaki kindly provided us with data of pen shell samples analyzed in Aramaki (2013). We would also like to express our gratitude to Drs. Kengo Suzuki, Takeo Kurihara and Takenori Sasaki for their useful suggestions. This work was supported by a grant from Japan Fisheries Research and Education Agency and Grants-in-Aid for Scientific Researches (Nos. 15K18731, 16K07859 and 18K11625) to K.H. and K.Y.


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Copyright information

© Japanese Society of Fisheries Science 2018

Authors and Affiliations

  1. 1.Seikai National Fisheries Research InstituteNagasakiJapan
  2. 2.Center for Water Cycle, Marine Environment, and Disaster ManagementKumamoto UniversityKumamotoJapan

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