Skip to main content
SpringerLink
Log in

Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in Pacific white shrimp Litopenaeus vannamei after multiple-dose oral administration

  • Original Article
  • Aquaculture
  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

The present study was designed to explore pharmacokinetics (PK) of enrofloxacin and its metabolite ciprofloxacin in Pacific white shrimp Litopenaeus vannamei after multiple-dose oral administration of enrofloxacin (30 mg/kg dose per 12 h and continuous feeding for 3 days). Enrofloxacin and ciprofloxacin concentrations in hemolymph, hepatopancreas, and muscle of the shrimp were simultaneously determined by high-performance liquid chromatography. PK parameters were analyzed based on statistical moment theory. Meanwhile, the relationship of pharmacokinetics/pharmacodynamics (PK/PD) was established based on drug concentration of hemolymph and in vitro antibacterial activity (MIC value). Results showed faster absorption of enrofloxacin in hemolymph (Tmax = 1 h) and muscles (Tmax = 1 h) than that in hepatopancreas (Tmax = 3 h) after the first oral administration. In multiple-dose oral administration, slight accumulation of enrofloxacin occurred in the hemolymph and hepatopancreas, while in the muscle, enrofloxacin concentration showed a significant decline following multiple administration. Tissue distribution of enrofloxacin and ciprofloxacin both followed the order hepatopancreas > hemolymph > muscle, with significantly higher ciprofloxacin concentration in hepatopancreas than in hemolymph (approximately 10-fold) and muscles (approximately 50-fold), indicating that the hepatopancreas is the main organ involved in metabolism of enrofloxacin in Pacific white shrimp. After multiple-dose administration, Cmax/MIC and AUC0–24/MIC values showed that the therapeutic regimen in this study could be remarkably effective in prevention and treatment of Vibrio infection in Pacific white shrimp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Aguirre-Guzmán G, Sánchez-Martínez JG, Pérez-Castañeda R, Palacios-Monzón A, Trujillo-Rodríguez T, Cruz-Hernández NIDL (2010) Pathogenicity and infection route of Vibrio parahaemolyticus in American White Shrimp, Litopenaeus vannamei. J World Aquac Soc 41:464–470

    Article  Google Scholar 

  • Barron MG, Hansen SC, Ball T (1991) Pharmacokinetics and metabolism of triclopyr in the crayfish (Procambarus clarki). Drug Metab Dispos Biol Fate Chem 19:163

    PubMed  CAS  Google Scholar 

  • Bergan T, Dalhoff PDA, Rohwedder R (1988) Pharmacokinetics of ciprofloxacin. Infection 16:S3–S13

    Article  PubMed  CAS  Google Scholar 

  • Della RG, Di SA, Malvisi J, Sello M (2004) The disposition of enrofloxacin in seabream (Sparus aurata L.) after single intravenous injection or from medicated feed administration. Aquaculture 232:53–62

    Article  CAS  Google Scholar 

  • Fang WH, Zhou S, Yu HJ, Hu LL, Zhou K, Liang SC (2007) Pharmacokinetics and tissue distribution of enrofloxacin and its metabolite ciprofloxacin in Scylla serrata following oral gavage at two salinities. Aquaculture 272:180–187

    Article  CAS  Google Scholar 

  • Gore SR, Harms CA, Kukanich B, Forsythe J, Lewbart GA, Papich MG (2005) Enrofloxacin pharmacokinetics in the European cuttlefish, Sepia officinalis, after a single i.v. injection and bath administration. J Vet Pharmacol Ther 28:433–439

    Article  PubMed  CAS  Google Scholar 

  • Han JE, Mohney LL, Tang KFJ, Pantoja CR, Lightner DV (2015) Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquac Rep 2:17–21

    Article  Google Scholar 

  • Intorre L, Cecchini S, Bertini S, Cognetti Varriale AM, Soldani G, Mengozzi G (2000) Pharmacokinetics of enrofloxacin in the seabass (Dicentrarchus labrax). Aquaculture 182:49–59

    Article  CAS  Google Scholar 

  • Jacobs MR (2001) Optimisation of antimicrobial therapy using pharmacokinetic and pharmacodynamic parameters. Clin Microbiol Infec Offl Publ Eur Soc Clin Microbiol Infect Dis 7:589–596

    CAS  Google Scholar 

  • Jr OR, Bhavnani SM, Ambrose PG (2005) Assessment of pharmacokinetic-pharmacodynamic target attainment of gemifloxacin against Streptococcus pneumoniae. Diagn Microbiol Infect Dis 51:45–49

    Article  CAS  Google Scholar 

  • Kalpana S, Aggarwal M, Srinivasa RG, Malik JK (2012) Effects of aflatoxin B1 on tissue residues of enrofloxacin and its metabolite ciprofloxacin in broiler chickens. Environ Toxicol Pharmacol 33:121–126

    Article  PubMed  CAS  Google Scholar 

  • Kim MS, Lim JH, Park BK, Hwang YH, Yun HI (2006) Pharmacokinetics of enrofloxacin in Korean catfish (Silurus asotus). J Vet Pharmacol Ther 29:397–402

    Article  PubMed  CAS  Google Scholar 

  • Koc F, Uney K, Atamanalp M, Tumer I, Kaban G (2009) Pharmacokinetic disposition of enrofloxacin in brown trout (Salmo trutta fario) after oral and intravenous administrations. Aquaculture 295:142–144

    Article  CAS  Google Scholar 

  • Lewbart G, Vaden S, Deen J, Manaugh C, Whitt D, Doi A, Smith T, Flammer K (1997) Pharmacokinetics of enrofloxacin in the red pacu (Colossoma brachypomum) after intramuscular, oral and bath administration. J Vet Pharmacol Therapeut 20:124–128

    Article  CAS  Google Scholar 

  • Liu CH, Cheng W, Hsu JP, Chen JC (2004) Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16S rDNA sequencing. Dis Aquat Organ 61:169

    Article  PubMed  CAS  Google Scholar 

  • Lode H, Borner K, Koeppe P (1998) Pharmacodynamics of fluoroquinolones. Clin Infect Dis Offl Publ Infect Dis Soc Am 27:33–39

    Article  CAS  Google Scholar 

  • Lomelí-Ortega CO, Martínez-Díaz SF (2014) Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae. Aquaculture 434:208–211

    Article  Google Scholar 

  • Ma R, Wang Y, Zou X, Hu K, Sun B, Fang W, Fu G, Yang X (2017a) Pharmacokinetics of sulfamethoxazole and trimethoprim in Pacific white shrimp, Litopenaeus vannamei, after oral administration of single-dose and multiple-dose. Environ Toxicol Pharmacol 52:90

    Article  PubMed  CAS  Google Scholar 

  • Ma R, Yang L, Ren T, Dong Y, Liu T, Zhuang P, Fang W, Yang X, Hu K (2017b) Enrofloxacin pharmacokinetics in Takifugu flavidus after oral administration at three salinity levels. Aquac Res 48:4545–4553

    Article  CAS  Google Scholar 

  • Park ED, Lightner DV, Milner N, Mayersohn M, Park DL, Gifford JM, Bell TA (1995) Exploratory bioavailability and pharmacokinetic studies of sulphadimethoxine and ormetoprim in the penaeid shrimp, Penaeus vannamei. Aquaculture 130:113–128

    Article  CAS  Google Scholar 

  • Phu TM, Douny C, Scippo ML, Pauw ED, Thinh NQ, Huong DTT, Vinh HP, Phuong NT, Dalsgaard A (2015) Elimination of enrofloxacin in striped catfish (Pangasianodon hypophthalmus) following on-farm treatment. Aquaculture 438:1–5

    Article  CAS  Google Scholar 

  • Rao GS, Ramesh S, Ahmad AH, Tripathi HC, Sharma LD, Malik JK (2002) Disposition kinetics of enrofloxacin and ciprofloxacin following intravenous administration of enrofloxacin in goats. Small Ruminant Res 44:9–15

    Article  Google Scholar 

  • Roque A, Gomez-Gil B (2003) Therapeutic effects of enrofloxacin in an experimental infection with a luminescent Vibrio harveyi in Artemia franciscana Kellog 1906. Aquaculture 220:37–42

    Article  CAS  Google Scholar 

  • Roque A, Molina-Aja A, Bolán-Mejı́ AC, Gomez-Gil B (2001) In vitro susceptibility to 15 antibiotics of vibrios isolated from penaeid shrimps in Northwestern Mexico. Int J Antimicrob Agents 17:383–387

    Article  PubMed  CAS  Google Scholar 

  • Sotorodriguez S, Armenta M, Gomezgil B (2006) Effects of enrofloxacin and florfenicol on survival and bacterial population in an experimental infection with luminescent Vibrio campbellii in shrimp larvae of Litopenaeus vannamei. Aquaculture 255:48–54

    Article  CAS  Google Scholar 

  • Stoffregen DA, Wooster GA, Bustos PS, Bowser PR, Babish JG (2010) Multiple route and dose pharmacokinetics of enrofloxacin in juvenile Atlantic salmon. J Vet Pharmacol Ther 20:111–123

    Article  Google Scholar 

  • Su Z, Hui X, Chen L (2011) In vitro antibacterial effect of chinese herbal medicine against Aeromonas hydrophila. Chin J Vet Med 134:233–239

    Google Scholar 

  • Tang J, Yang XL, Zheng ZL, Yu WJ, Hu K, Yu HJ (2006) Pharmacokinetics and the active metabolite of enrofloxacin in Chinese mitten-handed crab (Eriocheir sinensis). Aquaculture 260:69–76

    Article  CAS  Google Scholar 

  • Tyczkowska K, Hedeen KM, Aucoin DP, Aronson AL (1989) High-performance liquid chromatographic method for the simultaneous determination of enrofloxacin and its primary metabolite ciprofloxacin in canine serum and prostatic tissue. J Chromatogr 493:337–346

    Article  PubMed  CAS  Google Scholar 

  • Vaccaro E, Giorgi MV, Mengozzi G, Gervasi PG (2003) Inhibition of cytochrome P450 enzymes by enrofloxacin in the sea bass (Dicentrarchus labrax). Aquat Toxicol 62:27–33

    Article  PubMed  CAS  Google Scholar 

  • Watts JL (2008) Performance standards for antimicrobial disk and dilution susceptibilty tests for bacteria isolated from animals: approved standard. Clinical and Laboratory Standards Institute

  • Zhou J, Fang W, Yang X, Zhou S, Hu L, Li X, Qi X, Su H, Xie L (2012) A nonluminescent and highly virulent Vibrio harveyi strain is associated with “bacterial white tail disease” of Litopenaeus vannamei Shrimp. PLoS One 7:e29961

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported financially by National Key Research and Development Program of China (no. 2017YFC1600704), and Central Public-interest Scientific Institution Basal Research Fund, CAFS (no. 2017HY-ZD1006), and Shanghai Agriculture Applied Technology Development Program of China (no. G20140304).

Author information

Author notes

  1. Rongrong Ma, Liang Huang and Wenjuan Wei contributed equally to this study.

Authors and Affiliations

  1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, People’s Republic of China

    Rongrong Ma, Liang Huang & Wenjuan Wei

  2. National Pathogen Collection Center for Aquatic Animals, Shanghai, People’s Republic of China

    Rongrong Ma, Liang Huang & Wenjuan Wei

  3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, People’s Republic of China

    Rongrong Ma, Liang Huang & Wenjuan Wei

  4. Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Yangpu District, Shanghai, 200090, People’s Republic of China

    Rongrong Ma, Liang Huang, Wenjuan Wei, Yuan Wang, Xiong Zou, Junfang Zhou, Xincang Li & Wenhong Fang

Authors
  1. Rongrong Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenjuan Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiong Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Junfang Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xincang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wenhong Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wenhong Fang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest concerning this article.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ma, R., Huang, L., Wei, W. et al. Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in Pacific white shrimp Litopenaeus vannamei after multiple-dose oral administration. Fish Sci 84, 869–876 (2018). https://doi.org/10.1007/s12562-018-1229-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12562-018-1229-y

Keywords

Search

Navigation