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Fisheries Science

, Volume 84, Issue 3, pp 495–504 | Cite as

Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue

  • Kotaro Amezawa
  • Ryosuke Yazawa
  • Yutaka TakeuchiEmail author
  • Goro Yoshizaki
Original Article Aquaculture

Abstract

Scombridae species, such as tunas and mackerels, often do not spawn in land-based fish tanks without hormone treatment. To induce spawning in various fishes, a gonadotropin-releasing hormone analogue (GnRHa) is often administered by pellet implantation. Noninvasive administration is desired to induce spawning in scombrids that are sensitive to handling stress. Spawning induction by oral administration has been reported in several fishes, yet this method has not been put into practice in the aquaculture industry since a considerable amount of GnRHa is needed. Utilization of peptide synthesizers is widespread, and antigen-grade GnRHa (AgGnRHa) produced by a custom-peptide supplier is approximately 100-fold cheaper than conventional reagent-grade GnRHa (RgGnRHa), although the purity of AgGnRHa is lower. Here, we confirmed that the spawning induction potency of AgGnRHa was similar to that of RgGnRHa by pellet implantation in blue mackerel Scomber australasicus. Oral administration of AgGnRHa [6.0 mg/kg body weight (BW) per day] showed an equivalent ability to induce spawning of the mackerel as pellet implantation (0.1 mg/kg BW). We could also induce spawning of eastern little tuna Euthynnus affinis by oral administration of the AgGnRHa. Further, the obtained eggs showed higher survival. Thus, the oral delivery of AgGnRHa could be a powerful tool to induce spawning in Scombridae.

Keywords

Scombridae Teleosts Implant Egg survival Peptide synthesizer Reproductive dysfunction 

Notes

Acknowledgments

We thank the Oita Marine Biological Technology Center and Central Research Laboratory, Nippon Suisan Kaisha (Tokyo, Japan) and Marutou (Wakayama, Japan) for their assistance in supplying and transporting blue mackerel and ELT.

Supplementary material

12562_2018_1185_MOESM1_ESM.pptx (51 kb)
Supplementary material 1 (PPTX 51 kb)

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Copyright information

© Japanese Society of Fisheries Science 2018

Authors and Affiliations

  • Kotaro Amezawa
    • 1
  • Ryosuke Yazawa
    • 1
  • Yutaka Takeuchi
    • 2
    Email author
  • Goro Yoshizaki
    • 1
  1. 1.Department of Marine BiosciencesTokyo University of Marine Science and TechnologyTokyoJapan
  2. 2.Faculty of FisheriesKagoshima UniversityKagoshimaJapan

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