Abstract
Scombridae species, such as tunas and mackerels, often do not spawn in land-based fish tanks without hormone treatment. To induce spawning in various fishes, a gonadotropin-releasing hormone analogue (GnRHa) is often administered by pellet implantation. Noninvasive administration is desired to induce spawning in scombrids that are sensitive to handling stress. Spawning induction by oral administration has been reported in several fishes, yet this method has not been put into practice in the aquaculture industry since a considerable amount of GnRHa is needed. Utilization of peptide synthesizers is widespread, and antigen-grade GnRHa (AgGnRHa) produced by a custom-peptide supplier is approximately 100-fold cheaper than conventional reagent-grade GnRHa (RgGnRHa), although the purity of AgGnRHa is lower. Here, we confirmed that the spawning induction potency of AgGnRHa was similar to that of RgGnRHa by pellet implantation in blue mackerel Scomber australasicus. Oral administration of AgGnRHa [6.0 mg/kg body weight (BW) per day] showed an equivalent ability to induce spawning of the mackerel as pellet implantation (0.1 mg/kg BW). We could also induce spawning of eastern little tuna Euthynnus affinis by oral administration of the AgGnRHa. Further, the obtained eggs showed higher survival. Thus, the oral delivery of AgGnRHa could be a powerful tool to induce spawning in Scombridae.
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References
Donaldson EM, Hunter GA (1983) Induced final maturation, ovulation, and spermiation in cultured fish. Fish Physiol. https://doi.org/10.1016/s1546-5098(08)60307-6
King JA, Millar RP (1995) Evolutionary aspects of gonadotropin-releasing hormone and its receptor. Cell Mol Neurobiol. https://doi.org/10.1007/bf02069556
Matsuyama M, Takeuchi H, Kashiwagi M, Hirose K (1995) Induced gonadal development and spawning of immature red sea bream Pagrus major with LHRH-a administration in different ways during winter season. Fish Sci. https://doi.org/10.1016/0044-8486(88)90069-5
Mehdi Y, Ehsan S (2011) A review of the control of reproduction and hormonal manipulations in finfish species. Afr J Agric. https://doi.org/10.5897/ajar10.1080
Mendiola D, Yamashita Y, Matsuyama M, Alvarez P, Tanaka M (2008) Scomber japonicus, H is a better candidate species for juvenile production activities than Scomber scombrus L. Aquac Res. https://doi.org/10.1111/j.1365-2109.2008.01959.x
Mikolajczyk T, Roelants I, Epler P, Ollevier F (2002) Modified absorption of sGNRH-a following rectal and oral delivery to common carp, Cyprinus carpio L. Aquaculture. https://doi.org/10.1016/s0044-8486(01)00637-8
Milla S, Wang N, Mandiki M, Kestemont P (2009) Corticosteroids: friends or foes of teleost fish reproduction? Comp Biochem Physiol. https://doi.org/10.1016/j.cbpa.2009.02.027
Mylonas CC, Zohar Y (2000) Use of GnRHa-delivery systems for the control of reproduction in fish. Rev Fish Biol Fish. https://doi.org/10.1023/a:1012279814708
Mylonas CC, Bridges C, Gordin H, Ríos AB, García A, De La Gándara F, Fauve C, Suquet M, Medina A, Papadaki M, Heinisch G, De Metrio G, Corriero A, Vassallo-Agius R, Guzmán JM, Mañanos E, Zohar Y (2007) Preparation and administration of gonadotropin-releasing hormone agonist (GnRHa) implants for the artificial control of reproductive maturation in captive-reared atlantic bluefin tuna (Thunnus thynnus thynnus). Rev Fish Sci. https://doi.org/10.1080/10641260701484572
Mylonas CC, Fostier A, Zanuy S (2010) Brood stock management and hormonal manipulations of fish reproduction. Gen Comp Endocrinol. https://doi.org/10.1016/j.ygcen.2009.03.007
Mylonas CC, Mitrizakis N, Castaldo CA, Cerviño CP, Papadaki M, Sigelaki I (2013) Reproduction of hatchery-produced meagre Argyrosomus regius in captivity. II. Hormonal induction of spawning and monitoring of spawning kinetics egg production and egg quality. Aquaculture. https://doi.org/10.1016/j.aquaculture.2013.09.008
Okuzawa K, Amano M, Kobayashi M, Aida K, Hanyu I, Hasegawa Y, Miyamoto K (1990) Differences in salmon GnRH and chicken GnRH-II contents in discrete brain areas of male and female rainbow trout according to age and stage of maturity. Gen Comp Endocrinol. https://doi.org/10.1016/0016-6480(90)90155-F
Pankhurst NW (2011) The endocrinology of stress in fish: an environmental perspective. Gen Comp Endocrinol. https://doi.org/10.1016/j.ygcen.2010.07.017
Peter RE, Yu KL (1997) Neuroendocrine regulation of ovulation in fishes: basic and applied aspects. Rev Fish Biol Fish. https://doi.org/10.1023/a:1018431610220
Portz DE, Woodley CM, Cech JJ (2006) Stress-associated impacts of short-term holding on fishes. Rev Fish Biol Fish. https://doi.org/10.1007/s11160-006-9012-z
Rainis S, Ballestrazzi R (2005) The control of reproduction in finfish species through GnRH treatments. Ital J Anim Sci. https://doi.org/10.4081/ijas.2005.345
Schreck CB, Contreras-Sanchez W, Fitzpatrick MS (2001) Effects of stress on fish reproduction, gamete quality, and progeny. Aquaculture. https://doi.org/10.1016/S0044-8486(01)00580-4
Shiraishi T, Ohta K, Yamaguchi A, Yoda M, Chuda H (2005) Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity. Fish Sci. https://doi.org/10.1111/j.1444-2906.2005.00997.x
Shiraishi T, Ketkar SD, Kitano H, Nyuji M, Yamaguchi A, Matsuyama M (2008) Time course of final oocyte maturation and ovulation in chub mackerel Scomber japonicus induced by hCG and GnRHa. Fish Sci. https://doi.org/10.1111/j.1444-2906.2008.01587.x
Solar II, McLean E, Baker IJ, Sherwood NM, Donaldson EM (1990) Short communication: induced ovulation of sablefish (Anoplopoma fimbria) following oral administration of des Gly10-(D-Ala6)LH-RH ethylamide. Fish Physiol Biochem. https://doi.org/10.1007/bf00003406
Sorbera LA, Mylonas CC, Zanuy S, Carrillo M, Zohar Y (1996) Sustained administration of GnRHa increases milt volume without altering sperm counts in the sea bass. J Exp Zool. https://doi.org/10.1002/(SICI)1097-010X(19961201)276:5<361:AID-JEZ6>3.0.CO;2-M
Thomas P, Boyd NW (1989) Dietary administration of an LHRH analogue induces spawning of spotted sea trout (Cynoscion nebulosus). Aquaculture. https://doi.org/10.1016/0044-8486(89)90183-X
Yazawa R, Takeuchi Y, Amezawa K, Sato K, Iwata G, Kabeya N, Yoshizaki G (2015a) GnRHa-induced spawning of the eastern little tuna (Euthynnus affinis) in a 70-m3 land-based tank. Aquaculture. https://doi.org/10.1016/j.aquaculture.2015.01.016
Yazawa R, Takeuchi Y, Satoh K, Machida Y, Amezawa K, Kabeya N, Shimada Y, Yoshizaki G (2015b) Eastern little tuna, Euthynnus affinis (Cantor, 1849) mature and reproduce within 1 year of rearing in land-based tanks. Aquac Res. https://doi.org/10.1111/are.12831
Yazawa R, Takeuchi Y, Satoh K, Machida Y, Amezawa K, Kabeya N, Yoshizaki G (2016) Spawning induction and seed production of eastern little tuna, Euthynnus affinis (Cantor, 1849), in the post- and pre-spawning seasons by hormonal treatment in a semi-closed recirculation system with elevated temperature. Aquac Res. https://doi.org/10.1111/are.13173
Zohar Y, Mylonas CC (2001) Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture. https://doi.org/10.1016/s0044-8486(01)00584-1
Zohar Y, Goren A, Tosky M, Pagelson G, Leibovitz D, Koch Y (1989) The bioactivity of gonadotropin releasing hormones and its regulation in the gilthead seabream, Sparus aurata: in vivo and in vitro studies. Fish Physiol Biochem. https://doi.org/10.1007/bf00004690
Zohar Y, Muñoz-Cueto JA, Elizur A, Kah O (2010) Neuroendocrinology of reproduction in teleost fish. Gen Comp Endocrinol. https://doi.org/10.1016/j.ygcen.2009.04.017
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We thank the Oita Marine Biological Technology Center and Central Research Laboratory, Nippon Suisan Kaisha (Tokyo, Japan) and Marutou (Wakayama, Japan) for their assistance in supplying and transporting blue mackerel and ELT.
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Amezawa, K., Yazawa, R., Takeuchi, Y. et al. Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue. Fish Sci 84, 495–504 (2018). https://doi.org/10.1007/s12562-018-1185-6
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DOI: https://doi.org/10.1007/s12562-018-1185-6