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Fisheries Science

, Volume 83, Issue 3, pp 401–412 | Cite as

Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

  • Kogen Okita
  • Engkong Tan
  • Hina Satone
  • Shigeharu Kinoshita
  • Shuichi Asakawa
  • Daisuke Ojima
  • Hideki Yamazaki
  • Kazutaka Sakiyama
  • Tomohiro Takatani
  • Osamu Arakawa
  • Atsushi Hagiwara
  • Yoshitaka Sakakura
Original Article Biology
  • 499 Downloads

Abstract

To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.

Keywords

Takifugu rubripes Tetrodotoxin (TTX) Central nervous system RNA sequencing Feeding center Reward system 

Notes

Acknowledgements

This study was financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) to Y. S. (15K07581, 24380109, 21580227, 19580209), T. T. (26450287), and a Grant-in-Aid for JSPS Research Fellows from JSPS to K. O. (14J00997). This study was partly supported by the Nagasaki University Major Research Project to Y. S., T. T. and O. A.

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Copyright information

© Japanese Society of Fisheries Science 2017

Authors and Affiliations

  • Kogen Okita
    • 1
    • 5
  • Engkong Tan
    • 2
  • Hina Satone
    • 2
  • Shigeharu Kinoshita
    • 2
  • Shuichi Asakawa
    • 2
  • Daisuke Ojima
    • 3
  • Hideki Yamazaki
    • 3
  • Kazutaka Sakiyama
    • 4
  • Tomohiro Takatani
    • 1
  • Osamu Arakawa
    • 1
  • Atsushi Hagiwara
    • 1
  • Yoshitaka Sakakura
    • 1
  1. 1.Graduate School of Fisheries and Environmental SciencesNagasaki UniversityBunkyo 1-14Japan
  2. 2.Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyoJapan
  3. 3.Research Center for Marine Invertebrates, National Research Institute of Fisheries and Environment of Inland SeaJapan Fisheries Research and Education AgencyMomoshimaJapan
  4. 4.Japan Sea National Research Institute of FisheriesJapan Fisheries Research and Education AgencyMiyazuJapan
  5. 5.National Research Institute of Fisheries and Environment of Inland SeaJapan Fisheries Research and Education AgencyHatsukaichiJapan

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