Fisheries Science

, Volume 83, Issue 3, pp 455–464 | Cite as

Effects of starvation and feeding on blood chemistry, fatty acid composition and expression of vitellogenin and fatty acid-binding protein genes in female swimming crab Portunus trituberculatus broodstock

  • Liyun Ding
  • Huiyun Fu
  • Yingmei Hou
  • Min Jin
  • Peng Sun
  • Qicun Zhou
Original Article Aquaculture


Portunus trituberculatus broodstock were stocked in plastic tanks to evaluate the effects of starvation and feeding on gonadal development, blood chemistry, fatty acid composition, and expression of vitellogenin (Vtg) and fatty acid-binding protein genes (FABP) in females. Two treatments (starved and fed) were randomly assigned to triplicate groups of 90 swimming crab broodstock (approximately 230 ± 45 g). In the starved treatment, crabs were starved for 30 days, whereas in the fed treatment crabs were fed once a day with clams. The gonadosomatic index decreased significantly in starved crabs (P < 0.05), as did the serum glucose and cholesterol concentrations; conversely, the total protein concentration in serum significantly increased (P < 0.05). In the ovary, there was a significant relative decline of 18:0, 16:1n-7 and 20:1n-9 fatty acids and relative increases of 20:4n-6, 22:6n-3, 18:1n-9 and 20:5n-3 in starved crabs compared to fed crabs (P < 0.05). Relative expression of Vtg in the ovary decreased significantly in starved crabs (P < 0.05), while there was no significant difference in hepatopancreas Vtg expression between starved and fed crabs (P > 0.05). Starvation suppressed gonadal development in female swimming crab broodstock.


Gonadal development Glucose concentration Cholesterol concentration Crustacean Natural stressor Aquaculture 



This research was supported by the National Natural Science Foundation of China (41476125) and the Open Fund of Zhejiang Province (xkzsc1405). This research was also sponsored by the K. C. Wong Magna Fund and the K. C. Wong Education Foundation at Ningbo University. We would like to thank Y. W. Huo, M. Q. Wang, Z. M. Ren, T. L. Gao, S. K. Lu, Z. B. Yu, W. W. Huang, Y. Li and H. Qiu for their valuable help during the feeding trial and sample analysis.


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Copyright information

© Japanese Society of Fisheries Science 2017

Authors and Affiliations

  • Liyun Ding
    • 1
    • 2
  • Huiyun Fu
    • 2
  • Yingmei Hou
    • 1
  • Min Jin
    • 1
  • Peng Sun
    • 1
  • Qicun Zhou
    • 1
  1. 1.Laboratory of Fish Nutrition, School of Marine SciencesNingbo UniversityNingboChina
  2. 2.Jiangxi Fisheries Research InstituteNanchangChina

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