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Fisheries Science

, Volume 83, Issue 3, pp 465–477 | Cite as

Metabolic and phylogenetic profiles of microbial communities from a mariculture base on the Chinese Guangdong coast

  • Xiaojuan Hu
  • Guoliang Wen
  • Yucheng Cao
  • Yingxue Gong
  • Zhuojia Li
  • Zhili He
  • Yufeng Yang
Original Article Environment

Abstract

This study examined the phylogenetic and metabolic features of microbial communities in Baisha Bay mariculture areas of Nan’ao Island, a mariculture base on the Chinese Guangdong coast. Large seaweed (Gracilaria lemaneiformis) cultivation zones (LZ), fish culture zones (FZ), and control zones (CZ) were sampled. According to community-level physiological profiling, d-mannitol, d-mannitol/α-d-lactose, and d-mannitol/α-cyclodextrin were the carbon sources with the highest utilization rates in CZ, LZ, and FZ, respectively (R s  > 1.9). Denaturing gradient gel electrophoresis analysis showed that the dominant bacteria in CZ and LZ were Alphaproteobacteria (39.9 and 34.5%, respectively), whereas in FZ they were Flavobacteria (32.2%). The Shannon diversity in CZ and LZ was significantly higher than in FZ (P < 0.05). Some opportunistic pathogens, such as Vibrio, Pseudoalteromonas and Loktanella, were abundant in FZ. The microbial communities and most of the dominant bacteria in FZ were more affected by environmental factors including salinity, chemical oxygen demand and total phosphorus. Some bacteria, such as Brevundimonas and Polaribater, have the potential to degrade agar in seaweed, and were isolated from samples rich in algae which are dominant in LZ. The cultivation of G. lemaneiformis created the notable structures and functions of macroalgae-associated bacterial communities that were less affected by the environmental factors examined.

Keywords

Phylogeny Metabolism Large seaweed cultivation Fish culture Gracilaria lemaneiformis Aquaculture Community-level physiological profiling Denaturing gradient gel electrophoresis 

Notes

Acknowledgements

This research was supported by the Joint Project of the Nature Science Foundation of China-Guangdong (U1301235), the Chinese Special Fund for Agro-scientific Research in the Public Interest (201403008), the Fund of the Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, the Ministry of Agriculture, P. R. China (FREU2015-08), and the Natural Science Foundation of Hainan Province (20163148).

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Japanese Society of Fisheries Science 2017

Authors and Affiliations

  • Xiaojuan Hu
    • 1
    • 2
    • 3
  • Guoliang Wen
    • 1
  • Yucheng Cao
    • 1
  • Yingxue Gong
    • 4
  • Zhuojia Li
    • 1
  • Zhili He
    • 5
  • Yufeng Yang
    • 2
  1. 1.Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries InstituteChinese Academy of Fishery SciencesGuangzhouPeople’s Republic of China
  2. 2.Institute of HydrobiologyJinan UniversityGuangzhouPeople’s Republic of China
  3. 3.Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesSanyaPeople’s Republic of China
  4. 4.Institutes of Life and Health Engineering, College of Life Science and TechnologyJinan UniversityGuangzhouPeople’s Republic of China
  5. 5.Institute of Environmental Genomics and Department of Botany and MicrobiologyUniversity of OklahomaNormanUSA

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