Fisheries Science

, Volume 82, Issue 3, pp 509–517 | Cite as

Hepatic lipid accumulation is ameliorated in obese KK mice by dietary sei whale oil

  • Satoshi Hirako
  • Mamiko Suzuki
  • Hyounju Kim
  • Yuzuru Iizuka
  • Akiyo Matsumoto
  • Nobuhiro Wada
  • Mai Okabe
  • Fumiko Takenoya
  • Genta Yasunaga
  • Satoru Yamanaka
  • Akira Miyazaki
  • Seiji Shioda
Original Article Chemistry and Biochemistry

Abstract

Whale oil (WO) contains n-3 polyunsaturated fatty acids (PUFAs), but the effects of whale oil intake on lipid metabolism have remained unclear. We examined the influence of WO on lipid metabolism in obese KK mice. Male KK mice were fed a high-fat diet for 12 weeks to induce obesity. The mice were then given free access to a different diet for 10 weeks: the lard/safflower oil (LSO) diet consisted of 25 energy% (en%) LSO (6:4), the WO diet consisted of 25 en% WO, and the fish oil (FO) diet consisted of 11 en% FO plus 14 en% LSO. The n-3 PUFA content of the FO diet was the same as that in the WO diet. In the WO and FO groups, the total plasma cholesterol level was significantly decreased compared with that in the LSO group. The number of lipid droplets in liver specimens was also lower in the WO and FO groups, and the hepatic triglyceride and total cholesterol levels were decreased in these groups compared with the LSO group. Hepatic mRNA levels of fatty acid synthase, and stearoyl-CoA desaturase 1, which are fatty acid synthesis-related genes, were decreased in the WO and FO groups. No significant differences were observed between the WO and FO groups. Taken together, these results indicate that WO intake inhibits lipid accumulation in the liver by reducing fatty acid biosynthesis.

Keywords

Whale oil n-3 PUFA Lipid metabolism Hepatic lipids 

Abbreviations

ACC

Acyl-coA carboxylase

AOX

Acyl-CoA oxidase

CPT

Carnitine palmitoyltransferase

CT

Computed tomography

CYP7A1

Cholesterol 7 α-hydroxylase

DHA

Docosahexaenoic acid

DPA

Docosapentaenoic acid

d-ROM

Diacron of reactive oxygen metabolites

ELISA

Enzyme-linked immunosorbent assay

EPA

Icosapentaenoic acid

FAS

Fatty acid synthase

FO

Fish oil

GK

Glucokinase

HE

Hematoxylin-eosin

HDL

High-density lipoprotein

HMG-CoA

3-Hydroxy-3-methylglutaryl-coenzyme A

LSO

Lard/safflower oil

MCAD

Medium-chain acyl-CoA dehydrogenase

NAFLD

Nonalcoholic fatty liver disease

NASH

Nonalcoholic steatohepatitis

NEFA

Non-esterified fatty acid

PCR

Polymerase chain reaction

PEPCK

Phosphoenolpyruvate carboxykinase

PPAR

Peroxisome proliferator-activated receptor

PUFA

Polyunsaturated fatty acid

SCD

Stearoyl-CoA desaturase

SREBP

Sterol regulatory element-binding protein

U.CARR

Carratelli units

UCP

Uncoupling protein

WAT

White adipose tissue

WO

Whale oil

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Copyright information

© Japanese Society of Fisheries Science 2016

Authors and Affiliations

  • Satoshi Hirako
    • 1
  • Mamiko Suzuki
    • 2
  • Hyounju Kim
    • 3
  • Yuzuru Iizuka
    • 3
  • Akiyo Matsumoto
    • 3
  • Nobuhiro Wada
    • 4
  • Mai Okabe
    • 5
  • Fumiko Takenoya
    • 6
  • Genta Yasunaga
    • 7
  • Satoru Yamanaka
    • 2
  • Akira Miyazaki
    • 2
  • Seiji Shioda
    • 8
  1. 1.Department of Health and NutritionUniversity of Human Arts and SciencesSaitamaJapan
  2. 2.Department of BiochemistryShowa University School of MedicineTokyoJapan
  3. 3.Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical SciencesJosai UniversitySaitamaJapan
  4. 4.Department of Internal Medicine, Graduate School of MedicineThe University of TokyoTokyoJapan
  5. 5.Tokyo Shokuryo Dietitian AcademyTokyoJapan
  6. 6.Department of Exercise and Sports Physiology, School of Pharmacy and Pharmaceutical ScienceHoshi UniversityTokyoJapan
  7. 7.The Institute of Cetacean ResearchTokyoJapan
  8. 8.School of Pharmacy and Pharmaceutical Sciences, Global Research Center for Innovative Life Science Peptide Drug InnovationHoshi UniversityTokyoJapan

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