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Molecular cloning, tissue and embryonic development expression, and appetite regulatory effect of pancreatic peptide Y in Coilia nasus

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  • Chemistry and Biochemistry
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Abstract

Pancreatic peptide Y (PY) is a member of the neuropeptide Y family and is the result of duplication of the peptide YY gene, an anorexigenic neuropeptide that has been identified in mammals and some teleost species. The physiological function of PY in the regulation of appetite is not clear. In this study, highly conserved PY cDNA was characterized in Coilia nasus. The tissue distribution of C. nasus PY mRNA was analyzed by real-time PCR, demonstrating high levels of expression in the brain, intestine, and liver, but also with widespread distribution in other tissues. During embryogenesis, C. nasus PY mRNA was detected in early-stage developing embryos and was observed to increase throughout larval development. Expression of C. nasus PY mRNA initially decreased and then increased during fasting, suggesting a potential relationship between food intake and the expression of PY. This study has revealed a potential role for PY in appetite regulation and provides a basis for further study of PY in C. nasus.

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Acknowledgments

This study was supported by the Special Fund of the National Key Technology R&D Program (2012BAD26B05), the Key Technology R&D Program of Jiangsu Province (BE2014307), and the Three New Projects of Aquaculture Program of Jiangsu Province (D2015-14).

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Authors and Affiliations

  1. Fisheries College, Nanjing Agricultural University, Wuxi, 214128, China

    Siyu Yang, Gangchun Xu & Pao Xu

  2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, Jiangsu, China

    Gangchun Xu, Fukuan Du & Pao Xu

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  1. Siyu Yang
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  2. Gangchun Xu
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  4. Pao Xu
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Correspondence to Pao Xu.

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Yang, S., Xu, G., Du, F. et al. Molecular cloning, tissue and embryonic development expression, and appetite regulatory effect of pancreatic peptide Y in Coilia nasus . Fish Sci 82, 347–355 (2016). https://doi.org/10.1007/s12562-015-0960-x

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  • DOI: https://doi.org/10.1007/s12562-015-0960-x

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