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Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi

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  • Chemistry and Biochemistry
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Abstract

Growth hormone receptors (GHRs) are especially expressed in the muscle and liver tissues of mandarin fish Siniperca chuatsi. cDNAs of two GHR types (scGHR1 and scGHR2: 1999 and 1963 bp, encoding 638 and 578 amino acids, respectively) have been isolated and characterized in mandarin fish. Both receptors, formed through fish-specific genome duplication, have conserved FGEFS and box1 and 2 motifs. Partial compensatory growth was noted following 10-day re-feeding after 4-week fasting. After 1-week fasting, pituitary GH and muscle scGHR1 and scGHR2 mRNA levels and plasma GH concentrations increased (p < 0.05), while hepatic scGHR1 and scGHR2 mRNA expressions reduced rapidly (p < 0.05). Upon re-feeding, muscle scGHR1 and scGHR2 mRNA expressions decreased, whereas they increased rapidly in the liver, eventually normalizing. Pituitary GH mRNA expression increased to 5.1 times higher than that in the controls 3 days after re-feeding (p < 0.01), and normalized 7 days after re-feeding. The varying expression of scGHR1 and scGHR2 in different tissues suggests their distinct functions. Further, changes in GH, scGHR1, and scGHR2 mRNA expressions suggest their important roles in the growth of mandarin fish. Our results provide comparative insights into evolutionary origins and roles of GHR genes in teleost growth.

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Acknowledgments

This work was supported by the Educational Commission of Guangdong Province, China (No. cxzd1104), the Foshan Innovative and Entepreneurial Research Team Program (No. 2014IT100122), the Special Fund for Agro-scientific Research in the Public Interest (No. 201303048), the Science and Technology Planning Project of Guangdong Province (No. 2007A020300001-1; No. 2012A020800001; No. 2008A020100003), the Cooperative Project of Guangdong Province (No. 2011B090400179) and the Agriculture Science Technology Achievement Transformation project (No.2 012GB2E000338).

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Lu, X., Gu, Y., Hou, X. et al. Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi . Fish Sci 82, 155–169 (2016). https://doi.org/10.1007/s12562-015-0941-0

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