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Microsatellite DNA variation of oval squid Sepioteuthis sp. 2 reveals a single fishery stock on the coastline of mainland Japan

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Abstract

The level of genetic diversity and population differentiation of oval squid Shiro-ika Sepioteuthis sp. 2 around mainland Japan were examined using highly polymorphic DNA markers. Ten microsatellite loci showed relatively low genetic diversity (average observed heterozygosity H O = 0.68, overall mean number of alleles N A = 10.0) as compared with other squid, which may be explained by a recent history of population expansion. Populations from 12 sites along the coastline of Japan were genetically homogeneous, as measured by F ST values (global F ST: −0.0006; pairwise F ST: from −0.0046 to 0.0083), R ST values (global R ST: 0.0025; pairwise R ST: from −0.0076 to 0.03), a neighbor-joining (NJ) tree based on Cavalli-Sforza and Edwards chord distance, Bayesian clustering or analyses of three geographical groups (Sea of Japan, Seto Inland Sea, and Pacific Ocean; F CT = −0.0005), and should be considered as a single fishery stock. Allele frequencies also showed a high degree of similarity across different years in the samples taken from Hiroshima. Factors influencing this homogeneity include genetic exchange without barriers preventing gene flow, larval dispersal, and active adult migration of Sepioteuthis sp. 2.

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Acknowledgments

We gratefully acknowledge the assistance of people who helped with collecting specimens, Dr. Shiro Itoi (College of Bioresource Sciences, Nihon University), Mr. Atsushi Urabe (Kochi Prefectural Freshwater Fisheries Experimental Station), Dr. Akira Okamoto (Nagasaki Prefectural Government), Mr. Hiroyuki Hatanaka (Fukui Prefectural Fisheries Experimental Station), Ms. Shigemi Tsuda (Ishikawa Prefectural Fisheries Research Center), Mr. Kingo Tsumoto (Mie Prefectural Government), Mr. Kazuhiko Wada (Kagoshima Prefectural Government), and Mr. Makoto Watanabe (Miyazaki Prefectural Government). We would like to thank Dr. Tomoe Hikosaka for her cooperation and assistance in sequencing using the ABI 3130xl sequencer at the Gene Science Division, Natural Science Centre for Basic Research and Development, Hiroshima University. We also appreciate the cooperation of the executive committee of the squid fishing competition in Sukumo, Kochi Prefecture, and Breeden Co., Ltd., for kindly collecting specimens. This research was supported partly by Grants-in-Aid for Scientific Research (No. 26292106 for T.U.) from the Ministry of Education, Culture, Sport, Science, and Technology of Japan. This work was also supported by grants for a Research Fellowship from the Japan Society for Promotion of Science (JSPS) for Young Scientists to S.T.

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Authors and Affiliations

  1. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Satoshi Tomano, Gustavo Sanchez, Kanako Ueno & Tetsuya Umino

  2. Tokushima Agriculture, Forestry and Fisheries Technology Support Center, Naruto, Tokushima, 771-0361, Japan

    Yukio Ueta

  3. Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, Gero, Gifu, 509-2592, Japan

    Kenichi Ohara

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  1. Satoshi Tomano
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  2. Gustavo Sanchez
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  3. Kanako Ueno
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  4. Yukio Ueta
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  6. Tetsuya Umino
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Correspondence to Tetsuya Umino.

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Tomano, S., Sanchez, G., Ueno, K. et al. Microsatellite DNA variation of oval squid Sepioteuthis sp. 2 reveals a single fishery stock on the coastline of mainland Japan. Fish Sci 81, 839–847 (2015). https://doi.org/10.1007/s12562-015-0905-4

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  • DOI: https://doi.org/10.1007/s12562-015-0905-4

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