Fisheries Science

, Volume 80, Issue 3, pp 451–462 | Cite as

Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA)

  • Hee-Do Jeung
  • Do-Hyung Kang
  • Heung-Sik Park
  • Gilles Le Moullac
  • Kwang-Sik Choi
Original Article Biology

Abstract

We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to 18.4 %, while the GSI of the females collected from Tahiti ranged from 5.6 to 12.6 %. Similarly, the GSI of the male BLP oysters from Weno Island ranged from 0.8 to 8.5 %, while the GSI of the males from Tahiti ranged from 4.8 to 7.5 %. These results lead to the conclusion that the immunological probes developed in this study can be successfully applied to quantify BLP oyster gonadal tissues and that these probes can be used in studies of BLP oyster reproductive ecology based on their sensitivity, rapidity, and affordability.

Keywords

Black-lip pearl oyster ELISA Pinctada margaritifera Polyclonal antibody Quantification of eggs and sperm 

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Copyright information

© The Japanese Society of Fisheries Science 2014

Authors and Affiliations

  • Hee-Do Jeung
    • 1
  • Do-Hyung Kang
    • 2
  • Heung-Sik Park
    • 2
  • Gilles Le Moullac
    • 3
  • Kwang-Sik Choi
    • 1
  1. 1.School of Marine Biomedical ScienceJeju National UniversityJejuRepublic of Korea
  2. 2.Global Bioresources Research CenterKorea Institute of Ocean Science and Technology (KIOST)AnsanRepublic of Korea
  3. 3.Laboratoire Ecosystème PerlicoleIFREMER, Centre du PacifiqueTaravaoFrench Polynesia

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